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    LATERAL LOADING OF MICROCAPILLARY ARRAYS
    3.
    发明申请
    LATERAL LOADING OF MICROCAPILLARY ARRAYS 审中-公开

    公开(公告)号：WO2020118106A1

    公开(公告)日：2020-06-11

    申请号：PCT/US2019/064774

    申请日：2019-12-05

    申请人： XCELLA BIOSCIENCES, INC.

    发明人： CHEN, Bob ,  TAN, Ming, X. ,  ESCAJEDA, Arturo, M.

    IPC分类号： B01J19/00 ,  B01L3/00 ,  B81B1/00

    摘要： Lateral loading methods of use in high-throughput methods for screening large populations of variant proteins are provided. The methods utilize a flow cell encompassing large-scale arrays of microcapillaries, where each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules.

    METHODS AND SYSTEMS FOR SCREENING USING MICROCAPILLARY ARRAYS
    5.
    发明申请
    METHODS AND SYSTEMS FOR SCREENING USING MICROCAPILLARY ARRAYS 审中-公开

    公开(公告)号：WO2018111765A1

    公开(公告)日：2018-06-21

    申请号：PCT/US2017/065600

    申请日：2017-12-11

    申请人： XCELLA BIOSCIENCES, INC.

    发明人： COCHRAN, Jennifer, R. ,  CHEN, Bob ,  ALFORD, Spencer, Caleb

    IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N1/28 ,  G01N33/53 ,  G01N33/543

    摘要： High-throughput methods for screening large populations of variant proteins are provided. The methods utilize large-scale arrays of microcapillaries, where each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be isolated, and cells expressing the variant proteins of interest can be characterized. Also provided are systems for performing the disclosed screening methods.

    MULTI-STAGE SAMPLE RECOVERY SYSTEM
    8.
    发明申请
    MULTI-STAGE SAMPLE RECOVERY SYSTEM 审中-公开

    公开(公告)号：WO2018125832A1

    公开(公告)日：2018-07-05

    申请号：PCT/US2017/068296

    申请日：2017-12-22

    申请人： XCELLA BIOSCIENCES, INC.

    发明人： CHEN, Bob

    IPC分类号： C04B30/00 ,  C40B60/12 ,  G01N35/00 ,  G01N35/10

    摘要： Multi-stage sample-recovery systems, including automated 2-stage and 3- stage sample-recovery systems, are provided. Such systems enable the rapid screening and recovery of samples, including viable cell-based samples, from high- throughput screening systems, including systems utilizing large-scale arrays of microcapillaries. In specific screening systems, each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be identified and recovered using the multi-stage systems disclosed herein.