中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

397 records (took 0.02 seconds)

    CYTIDINE DEAMINASE INHIBITORS FOR THE TREATMENT OF PANCREATIC CANCER
    1.
    发明申请
    CYTIDINE DEAMINASE INHIBITORS FOR THE TREATMENT OF PANCREATIC CANCER 审中-公开
    CYDINE DEAMINASE抑制剂治疗胰腺癌

    公开(公告)号：WO2017158050A1

    公开(公告)日：2017-09-21

    申请号：PCT/EP2017/056160

    申请日：2017-03-15

    Applicant: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ,  UNIVERSITÉ PAUL SABATIER TOULOUSE III

    Inventor： CORDELIER, Pierre ,  GAYRAL, Marion

    IPC: A61K31/7068 ,  A61K31/7088 ,  A61K31/713 ,  A61K39/00 ,  A61K45/06 ,  A61P35/00 ,  G01N33/00 ,  G01N33/50 ,  C12Q1/25

    CPC classification number: A61K31/7115 ,  A61K31/7068 ,  A61K31/7088 ,  A61K31/713 ,  A61K45/06 ,  A61K48/005 ,  A61P35/00 ,  C12N15/62 ,  C12Q1/25 ,  G01N33/5011 ,  G01N2500/10

    Abstract: The present invention relates to methods and pharmaceutical compositions for use in the treatment of pancreatic cancer in a subject in need thereof. The inventors showed that targeting cytidine deaminase sensitizes cancer cells to chemotherapy (gemcitabine dFdC) both in vitro and in vivo in experimental models of PDA, with very high efficacy. To their surprise, CDA targeting in the absence of chemotherapy strongly alters cell proliferation and tumor progression. In particular, the present invention relates to a method for treating pancreatic cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a cytidine deaminase inhibitor in combination with an anti-pancreatic cancer treatment selected from the group consisting of CHK1 inhibitor, WEE1 inhibitor, ART inhibitor, DHODH inhibitor or gene therapy.

    Abstract translation: 本发明涉及用于在有需要的受试者中治疗胰腺癌的方法和药物组合物。 本发明人表明，在PDA的实验模型中，靶向胞苷脱氨酶在体外和体内都使癌细胞对化疗（吉西他滨dFdC）敏感，具有非常高的功效。 令他们惊讶的是，在没有化疗的情况下CDA靶向强烈改变细胞增殖和肿瘤进展。 具体而言，本发明涉及用于治疗有需要的受试者中的胰腺癌的方法，其包括向受试者施用治疗有效量的胞苷脱氨酶抑制剂以及选自以下的抗胰腺癌治疗剂：CHK1 抑制剂，WEE1抑制剂，ART抑制剂，DHODH抑制剂或基因疗法。

    DEVICES HAVING A SAMPLE DELIVERY COMPONENT
    2.
    发明申请
    DEVICES HAVING A SAMPLE DELIVERY COMPONENT 审中-公开
    具有样品传送组件的设备

    公开(公告)号：WO2017091213A1

    公开(公告)日：2017-06-01

    申请号：PCT/US2015/062426

    申请日：2015-11-24

    Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

    Inventor： MOLINE, Robert ,  GIRI, Manish ,  DOMINGUE, Chantelle

    IPC: G01N1/10 ,  G01N1/38 ,  G01N33/487 ,  G01N33/50 ,  G01N15/10 ,  G01N35/00 ,  B81B1/00 ,  B81C1/00

    CPC classification number: G01N15/0656 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0487 ,  C12Q1/25 ,  G01N1/38 ,  G01N33/48707 ,  G01N33/50 ,  G01N33/54366 ,  G01N2015/0065

    Abstract: Examples herein provide a device. The device includes a sample delivery component, which includes: a reagent chamber to contain at least one reagent; a sample chamber to contain a fluid sample; and a delivery channel extending from the reagent chamber and in fluid communication with the sample chamber and an output port, wherein the delivery channel is conducive to mixing the at least one reagent and the fluid sample to form a mixture before the mixture reaches the output port and be discharged therefrom. The device includes a testing cassette detachable from the delivery component, which includes: an input port in fluid communication with a microfluidic reservoir, the input port to receive the discharged fluid sample from the output port; and a micro-fabricated integrated sensor in a microfluidic channel extending from the microfluidic reservoir.

    Abstract translation: 这里的示例提供了一种装置。 该装置包括样品传送部件，其包括：用于容纳至少一种试剂的试剂室; 样品室以容纳流体样品; 以及从所述试剂室延伸并且与所述样品室和输出口流体连通的输送通道，其中所述输送通道有助于在所述混合物到达所述输出口之前混合所述至少一种试剂和所述流体样品以形成混合物 并从中排出。 该装置包括可从输送部件拆卸的测试盒，其包括：与微流体储存器流体连通的输入端口，输入端口用于从输出端口接收排出的流体样本; 以及从微流体储存器延伸的微流体通道中的微制造集成传感器。

    METHODS, COMPOSITIONS, AND SYSTEMS FOR CULTURING AND CHARACTERIZING FASTIDIOUS PLANT MICROBES
    3.
    发明申请
    METHODS, COMPOSITIONS, AND SYSTEMS FOR CULTURING AND CHARACTERIZING FASTIDIOUS PLANT MICROBES 审中-公开
    用于培养和表征紧急植物微生物的方法，组合物和系统

    公开(公告)号：WO2017087563A1

    公开(公告)日：2017-05-26

    申请号：PCT/US2016/062342

    申请日：2016-11-16

    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM

    Inventor： MANDADI, Kranthi, K. ,  IRIGOYEN, Sonia, C. ,  MIRKOV, T., Erik

    IPC: C12N1/20 ,  C12N15/82 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/68 ,  C07K14/415

    CPC classification number: C12N1/20 ,  A01H4/005 ,  A01H5/08 ,  A01H6/78 ,  C12N5/04 ,  C12N15/8241 ,  C12N2502/70 ,  C12Q1/025 ,  C12Q1/25 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/158 ,  G01N2333/195 ,  G01N2333/415

    Abstract: Numerous plant microbes, including the vascular-limited Candidatus spp. —causal agents of citrus greening and potato zebra chip diseases— are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system ( e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

    Abstract translation: 许多植物微生物，包括血管限制的Candidatus spp。 - 柑橘绿化和马铃薯斑马鱼病的致病因子 - 是不可培养的。 根据一些实施方案，本公开涉及用于培养这样的生物体的组合物，方法和系统。 例如，本公开涉及使用发根系统（例如体外，在植物中）培养，繁殖和表征苛刻的血管定居微生物的方法。 在一些实施方案中，本公开涉及用于培养苛养植物微生物的方法，包括：使由苛养植物微生物定殖的植物（例如，番茄植物，马铃薯植物，柑橘植物）接触（例如Xylella （Candidatus Liberibacter spp。））与发根根霉悬液混合，在允许诱导植物微生物繁殖发根的条件下，并繁殖定植的微生毛状根。

    BIOSENSORS THAT DETECT NAD+
    5.
    发明申请
    BIOSENSORS THAT DETECT NAD+ 审中-公开
    检测NAD +的生物传感器

    公开(公告)号：WO2016089633A1

    公开(公告)日：2016-06-09

    申请号：PCT/US2015/062003

    申请日：2015-11-20

    Applicant: OREGON HEALTH & SCIENCE UNIVERSITY

    Inventor： GOODMAN, Richard ,  COHEN, Michael ,  CAMBRONNE, Lulu ,  STEWART, Melissa

    IPC: G01N33/68 ,  G01N15/14 ,  G01N21/64 ,  C12N15/63 ,  C07K7/08 ,  C07K14/00 ,  C07K19/00

    CPC classification number: C12Q1/25 ,  C12N9/93 ,  C12Q1/008 ,  C12Y605/01002 ,  G01N2333/9015

    Abstract: A polypeptide biosensor that detects free NAD + is disclosed. The polypeptide comprises a first fragment from an NAD + dependent DNA ligase acetylation domain, a second fragment from the NAD + dependent DNA ligase acetylation domain, and a fluorescent protein, wherein the fluorescent protein is positioned between the two DNA ligase acetylation domain fragments. Also disclosed are expression vectors comprising the biosensor as well as methods of using the biosensor to detect NAD + .

    Abstract translation: 公开了检测游离NAD +的多肽生物传感器。 多肽包含来自NAD +依赖性DNA连接酶乙酰化结构域的第一片段，来自NAD +依赖性DNA连接酶乙酰化结构域的第二片段和荧光蛋白，其中所述荧光蛋白位于两个DNA连接酶乙酰化结构域片段之间。 还公开了包含生物传感器的表达载体以及使用生物传感器检测NAD +的方法。

    METHOD AND SYSTEM FOR DETERMINING A BIOLOGICAL RESPONSE OF A TARGET TO A SOLUBLE CANDIDATE SUBSTANCE
    8.
    发明申请
    METHOD AND SYSTEM FOR DETERMINING A BIOLOGICAL RESPONSE OF A TARGET TO A SOLUBLE CANDIDATE SUBSTANCE 审中-公开
    用于确定目标到可溶性候选物质的生物反应的方法和系统

    公开(公告)号：WO2014135512A1

    公开(公告)日：2014-09-12

    申请号：PCT/EP2014/054117

    申请日：2014-03-04

    Applicant: F. HOFFMANN-LA ROCHE AG

    Inventor： WERNER, Michael ,  MARTIN, Rainer E. ,  HOCHSTRASSER, Remo

    IPC: G01N33/536 ,  G01N33/558 ,  B01L3/00

    CPC classification number: G01N33/536 ,  B01L3/502776 ,  B01L2200/0694 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0472 ,  B01L2400/0487 ,  C12Q1/25 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/558 ,  G01N2021/6439 ,  Y10T436/25625 ,  Y10T436/2575

    Abstract: A method for determining a biological response of a target (41, 42) to a soluble candidate substance comprises the steps: introducing a soluble candidate substance into a laminar flow of a buffer liquid (2) to form a candidate substance solute (3) having an initial concentration profile (31); dispersing the initial concentration profile (31) to form a dispersed concentration profile (32); directing the dispersed concentration profile (32) into a detection channel (12) to form a final symmetrical concentration profile (33) therein; introducing a target into the detection channel (12) to obtain a combined concentration profile comprising a constant target concentration profile overlying the final symmetrical concentration profile (33); holding in the detection channel (12) at least one half of the combined concentration profile; and optically scanning the combined concentration profile to detect an optical signal representative of the biological response of the target to the soluble candidate substance.

    Abstract translation: 用于确定靶（41,42）对可溶性候选物质的生物反应的方法包括以下步骤：将可溶性候选物质引入缓冲液体（2）的层流中以形成候选物质溶质（3），其具有 初始浓度分布（31）; 分散初始浓度分布（31）以形成分散浓度分布（32）; 将分散的浓度分布（32）引导到检测通道（12）中以在其中形成最终对称的浓度分布（33）; 将目标引入检测通道（12）以获得组合浓度分布，其包括覆盖最终对称浓度分布（33）的恒定目标浓度分布; 在所述检测通道（12）中保持所述组合浓度分布的至少一半; 并且光学扫描组合的浓度分布，以检测表示靶向可溶性候选物质的生物反应的光学信号。

    MIRNA ANALYSIS METHODS
    10.
    发明申请
    MIRNA ANALYSIS METHODS 审中-公开
    MIRNA分析方法

    公开(公告)号：WO2013122996A1

    公开(公告)日：2013-08-22

    申请号：PCT/US2013/025860

    申请日：2013-02-13

    Applicant: THE JOHNS HOPKINS UNIVERSITY ,  WANG, Tza-Huei ,  LIU, Kelvin J. ,  SONG, Yunke

    Inventor： WANG, Tza-Huei ,  LIU, Kelvin J. ,  SONG, Yunke

    IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/58 ,  G01N33/50

    CPC classification number: C12Q1/6862 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/25 ,  C12Q1/6813 ,  G01N2333/9015 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2565/125 ,  C12Q2521/501 ,  C12Q2537/143

    Abstract: The present invention provides a PCR-free, multiplexed ligation assay for miRNA expression analysis that produces highly quantitative, 10-100 plex miRNA profiling in a single reaction. The inventive methods use a 2-step ligation assay to generate an array of miRNA specific ligation products that can be decoded and quantified by a size discrimination method such as gel electrophoresis or single molecule separation. One embodiment is a low-cost assay that can be performed using standard tools available in nearly all molecular biology laboratories. This assay requires nothing more than a gel apparatus and reader for detection. Other embodiments include use of magnetic beads and other size exclusion apparatus which give increasingly higher sensitivity, lower sample consumption, and reduced processing steps.

    Abstract translation: 本发明提供了一种用于miRNA表达分析的无PCR，多重连接测定法，其在单个反应中产生高度定量的10-100plex miRNA分析。 本发明的方法使用2步连接测定来产生miRNA特异性连接产物的阵列，其可以通过诸如凝胶电泳或单分子分离的尺寸鉴别方法进行解码和定量。 一个实施方案是可以使用几乎所有分子生物学实验室中可用的标准工具进行的低成本测定。 该测定仅需要用于检测的凝胶装置和读取器。 其他实施方案包括使用磁珠和其它尺寸排除装置，其提供越来越高的灵敏度，更低的样品消耗和减少的加工步骤。

Patent Agency Ranking