公开(公告)号：WO2012056322A1

公开(公告)日：2012-05-03

申请号：PCT/IB2011/002895

申请日：2011-10-27

Applicant: ZALESKI, Henryk ,  PINIARSKI, Mark ,  FELDBERG, Simon ,  ANDERSON, Jeff ,  SAMARIN, Oleg

Inventor： ZALESKI, Henryk ,  PINIARSKI, Mark ,  FELDBERG, Simon ,  ANDERSON, Jeff ,  SAMARIN, Oleg

IPC: H01J49/06 ,  H01J3/04 ,  H01J49/40

CPC classification number: G01N27/622 ,  G01N23/00 ,  G06K9/0053 ,  H01J49/0031 ,  H01J49/025 ,  H01J49/04 ,  H01J49/06 ,  H01J49/061

Abstract: Systems and methods disclosed provide for methods of managing polarity switching in an ion mobility spectrometer, and provide for management of the repelling grid voltage, the gating grid voltage, and the fixed grid voltage during polarity switching. Systems and methods also provide for the management of the effect of dielectric relaxation in an insulator proximal to the collector, and provide for a preamplifier coupled to the collector including a switch, and a method of managing the collector output including the switch. Systems and methods consistent with the current disclosure further provide for a method of normalizing ion mobility data by determining fitting coefficients associated with a plurality of measurement data sets, and subtracting the curves determined by the fitting coefficients from the data acquired by the ion mobility spectrometer.

Abstract translation: 所公开的系统和方法提供了在离子迁移谱仪中管理极性切换的方法，并且在极性切换期间提供排斥电网电压，门控电网电压和固定电网电压的管理。 系统和方法还提供了在靠近集电极的绝缘体中对介电弛豫的影响的管理，并且提供耦合到集电器的前置放大器，包括开关，以及管理包括开关的集电极输出的方法。 与本公开一致的系统和方法进一步提供了通过确定与多个测量数据集相关联的拟合系数来归一化离子迁移率数据的方法，以及从由离子迁移谱仪获取的数据中减去由拟合系数确定的曲线。

公开(公告)号：WO1997040939A1

公开(公告)日：1997-11-06

申请号：PCT/CA1997000302

申请日：1997-05-01

Applicant: VISIBLE GENETICS INC. ,  DUNN, James, M. ,  LEUSHNER, James ,  RENFREW, John, A. ,  WATERHOUSE, Paul ,  IZMAILOV, Alexandre M. ,  ZALESKI, Henryk

Inventor： VISIBLE GENETICS INC.

IPC: B01L07/00

CPC classification number: B01L7/52 ,  B01L7/525 ,  G01N2035/00237

Abstract: An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reaction vessels, such as capillary tubes, for the preparation and thermal cycling of reaction mixtures. In order to prevent loss of the reaction mixture from the vessels during heating, the thermal cycling apparatus of the invention provides means for reversibly sealing the proximal and distal ends of each reaction vessel. The proximal ends can be sealed by coupling to a pump which permits movement of the samples within the reaction vessels. As to the distal ends, the reaction vessels can be sealed by pressing the distal end of each vessel against a sealing element with a conformable surface, or by immersing the distal end of each vessel in the reservoir of liquid, preferably of an oil, that is not miscible with the reaction mixture.

Abstract translation: 在反应容器中热循环反应混合物以将混合物暴露于例如实现PCR扩增所需的变化温度或使用循环测序操作制备测序片段的装置和方法使用流通反应容器 ，如毛细管，用于反应混合物的制备和热循环。 为了防止加热期间反应混合物从容器中的损失，本发明的热循环装置提供了用于可逆地密封每个反应容器的近端和远端的装置。 近端可以通过联接到允许样品在反应容器内移动的泵来密封。 对于远端，可以通过将每个容器的远端压靠具有适形表面的密封元件，或通过将每个容器的远端浸入液体储存器（优选油）中来密封反应容器， 与反应混合物不混溶。

公开(公告)号：WO1997021995A1

公开(公告)日：1997-06-19

申请号：PCT/CA1996000832

申请日：1996-12-12

Applicant: VISIBLE GENETICS INC. ,  IZMAILOV, Alexandre ,  WATERHOUSE, Paul ,  ZALESKI, Henryk

Inventor： VISIBLE GENETICS INC.

IPC: G01N27/447

CPC classification number: G01N27/44721 ,  G01N27/44704

Abstract: Gel holders for electrophoresis gels are made using clad fibers, particularly glass fibers as spacers between substrates. A plurality of fibers with a high-melting interior core and a low-melting external cladding are placed between a first planar substrate and a second planar substrate. The fibers are heated to a temperatrure sufficient to at least soften the exterior cladding of the fibers without softening the interior core of the fibers, and then cooled while they are in contact with the first and second substrates to resolidify the exterior cladding. This adheres the fibers to the first and second substrates, and forms a gel chamber between said first and second substrates. The gel chamber has a thickness defined by interior core of the fibers. The fibers may be heated before or after the second substrate is placed over the top of the fibers. The gel holders thus formed may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

Abstract translation: 电泳凝胶的凝胶保持器使用包覆纤维制成，特别是作为衬底之间的间隔物的玻璃纤维。 具有高熔点内芯和低熔点外包层的多根纤维被放置在第一平面基板和第二平面基板之间。 纤维被加热到足以至少软化纤维的外包层的温度，而不软化纤维的内芯，然后在与第一和第二基底接触的同时冷却，以重新固化外包层。 这将纤维粘附到第一和第二基底上，并在所述第一和第二基底之间形成凝胶室。 凝胶室具有由纤维的内芯限定的厚度。 纤维可以在将第二基底放置在纤维顶部之前或之后被加热。 如此形成的凝胶保持器可立即用诸如聚丙烯酰胺的凝胶形成溶液填充，或者可以无限期地储存并根据需要使用。

公开(公告)号：WO2012172436A2

公开(公告)日：2012-12-20

申请号：PCT/IB2012001669

申请日：2012-06-15

Applicant: SMITHS DETECTION MONTREAL INC ,  LEVIN DANIEL ,  SERGEYEV VLAD ,  BONDARENKO VOLODIMIR ,  ATAMANCHUK BOHDAN ,  BIAN QUNZHOU ,  ZALESKI HENRYK ,  PINIARSKI MARK ,  FELDBERG SIMON ,  JACKSON RONALD

Inventor： LEVIN DANIEL ,  SERGEYEV VLAD ,  BONDARENKO VOLODIMIR ,  ATAMANCHUK BOHDAN ,  BIAN QUNZHOU ,  ZALESKI HENRYK ,  PINIARSKI MARK ,  FELDBERG SIMON ,  JACKSON RONALD

IPC: G01N27/68 ,  H01J49/40

CPC classification number: G01N27/622 ,  H01J49/12 ,  H01J49/168

Abstract: Looped ionization sources for ion mobility spectrometers are described. The ionization sources can be used to ionize molecules from a sample of interest in order to identify the molecules based on the ions. In an implementation, an electrical ionization source includes a wire that is looped between electrical contacts. The wire is used to form a corona responsive to application of voltage between the wire and the walls of an ionization chamber. The corona can form when a sufficient voltage is applied between the wire and the walls. A difference in electrical potential between the wire and a wall forming an ionization chamber, in which wire is contained, can be used to draw the ions away from the wire. In embodiments, the wire can be heated to reduce the voltage used to strike the corona. The ions, subsequently, may ionize the molecules from the sample of interest. The looped corona source can also be used in mass spectrometers (MS).

Abstract translation: 描述了用于离子迁移率光谱仪的环形电离源。 电离源可用于从感兴趣的样品中离子化分子，以便基于离子鉴定分子。 在一个实施方案中，电离电离源包括在电触头之间环绕的导线。 导线用于响应于在电线和电离室的壁之间施加电压而形成电晕。 当电线和墙壁之间施加足够的电压时，可以形成电晕。 电线和形成电离室的壁之间的电位差可以用于将离子从电线中拉出。 在实施例中，可以加热导线以降低用于击打电晕的电压。 随后，离子可以使目标样品中的分子离子化。 环状电晕源也可用于质谱仪（MS）。

公开(公告)号：WO1997021996A1

公开(公告)日：1997-06-19

申请号：PCT/CA1996000833

申请日：1996-12-12

Applicant: VISIBLE GENETICS INC. ,  IZMAILOV, Alexandre ,  ZALESKI, Henryk ,  WATERHOUSE, Paul

Inventor： VISIBLE GENETICS INC.

IPC: G01N27/447

CPC classification number: G01N27/44704

Abstract: Gel holders for electrophoresis gels are made using fibers, particularly glass fibers, which are affixed to the substrates forming the gel holder using an adhesive. These gel holders can be made by placing a plurality of adhesive-coated fibers between a first planar substrate and a second planar substrate; and applying pressure to the outside of the substrates to adhere the fibers to the first and second substrates. This forms a gel chamber between the first and second substrates which has a thickness defined by diameter of the fibers. Alternatively, uncoated fibers may be laid down in pairs, with a line of adhesive disposed between each fiber of the pair. When the adhesive is cured, it binds the fibers in position as spacers. At the same time, the fibers isolate the adhesive from the gel compartment. In this way, interference of components of the adhesive with the polymerization of the gel in the gel chamber can be avoided. Gel holders formed using either of these methods may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

Abstract translation: 用于电泳凝胶的凝胶保持器使用纤维，特别是玻璃纤维制成，其使用粘合剂固定到形成凝胶保持器的基材上。 这些凝胶保持器可以通过在第一平面基板和第二平面基板之间放置多个粘合剂涂覆的纤维来制造; 并向衬底外部施加压力以将纤维粘附到第一和第二衬底上。 这在第一和第二基底之间形成凝胶室，其具有由纤维的直径限定的厚度。 或者，未涂覆的纤维可以成对铺设，一对粘合剂布置在该对的每个纤维之间。 当粘合剂固化时，其将纤维作为间隔物结合在适当位置。 同时，纤维将胶粘剂与凝胶隔离隔离。 以这种方式，可以避免粘合剂组分与凝胶室中的凝胶聚合的干扰。 使用这些方法之一形成的凝胶容器可以立即用诸如聚丙烯酰胺的凝胶形成溶液填充，或者可以无限期地储存并根据需要使用。

公开(公告)号：WO2012172436A3

公开(公告)日：2012-12-20

申请号：PCT/IB2012/001669

申请日：2012-06-15

Applicant: SMITHS DETECTION MONTREAL INC. ,  LEVIN, Daniel ,  SERGEYEV, Vlad ,  BONDARENKO, Volodimir ,  ATAMANCHUK, Bohdan ,  BIAN, Qunzhou ,  ZALESKI, Henryk ,  PINIARSKI, Mark ,  FELDBERG, Simon ,  JACKSON, Ronald

Inventor： LEVIN, Daniel ,  SERGEYEV, Vlad ,  BONDARENKO, Volodimir ,  ATAMANCHUK, Bohdan ,  BIAN, Qunzhou ,  ZALESKI, Henryk ,  PINIARSKI, Mark ,  FELDBERG, Simon ,  JACKSON, Ronald

IPC: G01N27/68 ,  H01J27/14 ,  H01J49/12 ,  H01J49/40

Abstract: Looped ionization sources for ion mobility spectrometers are described. The ionization sources can be used to ionize molecules from a sample of interest in order to identify the molecules based on the ions. In an implementation, an electrical ionization source includes a wire that is looped between electrical contacts. The wire is used to form a corona responsive to application of voltage between the wire and the walls of an ionization chamber. The corona can form when a sufficient voltage is applied between the wire and the walls. A difference in electrical potential between the wire and a wall forming an ionization chamber, in which wire is contained, can be used to draw the ions away from the wire. In embodiments, the wire can be heated to reduce the voltage used to strike the corona. The ions, subsequently, may ionize the molecules from the sample of interest. The looped corona source can also be used in mass spectrometers (MS).

公开(公告)号：WO1998008978A1

公开(公告)日：1998-03-05

申请号：PCT/US1997015056

申请日：1997-08-27

Applicant: VISIBLE GENETICS INC. ,  WATERHOUSE, Paul ,  IZMAILOV, Alexandre, M. ,  ZALESKI, Henryk ,  YAGER, Thomas, D. ,  DUNN, James, M. ,  LEUSHNER, James ,  HUI, May ,  LARSON, Marina, T.

Inventor： VISIBLE GENETICS INC.

IPC: C12Q01/68

CPC classification number: B01L7/52 ,  G01N27/44743

Abstract: An apparatus for processing samples containing DNA to produce a sequencing fragment mixture comprises a sample processing element comprising: a thermocycling region having one or more chambers for receiving a DNA sequencing reaction mixture and forming sequencing fragments therefrom; a separation region comprising a separation matrix for separating the sequencing fragments formed in the thermocycling regions; a detection region for detection of the separated sequencing fragments; and means for regulating the temperature in the thermocycling region of the sample processing element to provide a plurality of thermal cycles, each cycle including at least a denaturation phase and an extension phase. The apparatus for processing sample can be placed in a holder which is associated with means for applying an electric field to the separation region of a sample processing apparatus placed within the holder to cause polynucleotide sequencing fragments to migrate through the separation region from the thermocycling region to the detection region; and means for detecting polynucleotide fragments within the detection region of the sample processing apparatus placed within the holder.

Abstract translation: 用于处理含有DNA的样品以产生测序片段混合物的装置包括样品处理元件，其包括：热循环区域，其具有用于接收DNA测序反应混合物并形成测序片段的一个或多个室; 分离区域，其包含用于分离在所述热循环区域中形成的测序片段的分离基质; 用于检测分离的测序片段的检测区域; 以及用于调节样品处理元件的热循环区域中的温度以提供多个热循环的装置，每个循环至少包括变性相和延伸相。 用于处理样品的装置可以放置在与将电场施加到放置在保持器内的样品处理装置的分离区域的装置相关联的保持器中，以使得多核苷酸测序片段通过分离区域从热循环区域迁移到 检测区域; 以及用于检测放置在保持器内的样品处理装置的检测区域内的多核苷酸片段的装置。

公开(公告)号：WO1996013717A1

公开(公告)日：1996-05-09

申请号：PCT/US1995014531

申请日：1995-10-31

Applicant: VISIBLE GENETICS INC. ,  RENFREW, John, A. ,  WATERHOUSE, Paul ,  STEVENS, John, K. ,  ZALESKI, Henryk

Inventor： VISIBLE GENETICS INC.

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  G01N27/44704 ,  G01N27/44747

Abstract: An electrophoresis microgel is formed in a gel holder. The gel holder comprises a top substrate, a bottom substrate and a spacer disposed between the top substrate and the bottom substrate. The spacer establishes a separation of from 25 to 250 microns between the top substrate and the bottom substrate. A gel compartment is formed by partially sealing the top substrate to the bottom substrate, while leaving an opening for the introduction of unpolymerized gel. The gel compartment is then filled with an unpolymerized gel, which is polymerized in the gel compartment. Electrodes may be printed on the substrates, may be contacted to an exposed edge of gel, or may be applied through windows cut into one of the substrates. One type of gel holder makes use of graded beads having a diameter of 25 to 250 microns slurried in an adhesive such as an acrylate adhesive as the spacer. The slurry is printed onto the surface of one or both substrates to form a spacer of the desired shape, and then hardened using heat or light. If desired, the spacer can establish lanes within the gel.

Abstract translation: 在凝胶保持器中形成电泳微凝胶。 凝胶保持器包括顶部基底，底部基底和设置在顶部基底和底部基底之间的间隔物。 间隔物在顶部基底和底部基底之间建立了从25至250微米的间隔。 通过将顶部基底部分地密封到底部基底而形成凝胶室，同时留下用于引入未聚合凝胶的开口。 然后用凝胶隔室填充未聚合的凝胶，其在凝胶隔室中聚合。 电极可以印刷在基底上，可以与暴露的凝胶边缘接触，或者可以通过切割成一个基底的窗口来施加。 一种类型的凝胶保持器使用在诸如丙烯酸酯粘合剂的粘合剂中作为间隔物浆化的直径为25至250微米的分级珠粒。 将浆料印刷到一个或两个基材的表面上以形成所需形状的间隔物，然后使用热或光硬化。 如果需要，间隔物可以在凝胶内建立泳道。

公开(公告)号：WO1996013715A1

公开(公告)日：1996-05-09

申请号：PCT/US1995013955

申请日：1995-10-31

Applicant: VISIBLE GENETICS INC. ,  RENFREW, John, A. ,  STEINBACH, Eric ,  STEVENS, John, K. ,  ZALESKI, Henryk

Inventor： VISIBLE GENETICS INC.

IPC: G01N27/44

CPC classification number: G01N27/44704

Abstract: An apparatus for the rapid preparation of electrophoresis gels comprises: a) a housing; b) a support fixture removably disposed within the housing and adapted to receive a gel holder having an internal gel compartment, the support fixture being optionally adapted to permit filling of the gel holder within the housing; c) an optional injection system, which is connectible to a reservoir for holding a polymerizable solution; d) an optional solution connector adapted to couple the injection system to a gel holder placed within the filling fixture; e) an optional controller for the injection system, which causes the injection system to inject polymerizable solution from the reservoir into the gel compartment; and f) a radiation source disposed within the housing in a location effective to irradiate polymerizable solution within the gel compartment of a gel holder in the support fixture. The support fixture may be mounted on a drawer which is slidable between a position inside the housing, and a position outside the housing to permit easy placement of a gel holder into the support fixture.

Abstract translation: 用于快速制备电泳凝胶的装置包括：a）壳体; b）可移除地设置在所述壳体内并且适于接收具有内部凝胶隔室的凝胶保持器的支撑固定件，所述支撑固定件可选地适于允许在所述壳体内填充所述凝胶保持器; c）可选的注射系统，其可连接到储存器以保持可聚合溶液; d）可选的解决方案连接器，其适于将注射系统耦合到放置在填充夹具内的凝胶保持器; e）用于注射系统的可选控制器，其使得注射系统将可聚合溶液从储器注入凝胶室; 以及f）辐射源，其设置在所述壳体内的位置，以有效地照射所述支撑夹具中的凝胶保持器的凝胶室内的可聚合溶液。 支撑固定件可以安装在可在壳体内的位置和壳体外部的位置之间滑动的抽屉上，以允许将凝胶保持器容易地放置到支撑夹具中。