公开(公告)号：WO2011009073A1

公开(公告)日：2011-01-20

申请号：PCT/US2010/042323

申请日：2010-07-16

Applicant: ABBOTT LABORATORIES ,  GRACE, John, P. ,  DIETRICH, Dimo ,  FASSBENDER, Anne ,  SCHATZ, Philipp ,  SOLOMON, Natalie ,  TETZNER, Reimo

Inventor： GRACE, John, P. ,  DIETRICH, Dimo ,  FASSBENDER, Anne ,  SCHATZ, Philipp ,  SOLOMON, Natalie ,  TETZNER, Reimo

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2527/107 ,  C12Q2527/101

Abstract: The invention here concerns a method for increasing the uniformity of nucleic acid amplification. A method is described to add stopping periods during the ramp step(s), which minimizes the effect of temperature overshoot or undershoot. Many cyclers' algorithms cause the target temperature to be passed during ramp and then returned to within a short period of time. This overshoot or undershoot can cause non-uniformity between reactions in a micro¬ liter plate. The addition of stopping periods reduces the effects of overshoot or undershoot and increases uniformity between different reactions wells on a plate.

Abstract translation: 本发明涉及增加核酸扩增的均匀性的方法。 描述了在斜坡步骤期间增加停止时间的方法，其使温度过冲或下冲的影响最小化。 许多循环仪的算法使得目标温度在斜坡期间通过，然后在短时间内返回。 这种过冲或下冲可能导致微升板之间的反应之间的不均匀。 加入停止时间可减少过冲或下冲的影响，并增加板上不同反应孔之间的均匀性。

公开(公告)号：WO1996010747A1

公开(公告)日：1996-04-11

申请号：PCT/US1995012462

申请日：1995-09-29

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HANSMANN, Douglas, D. ,  GRACE, John, P. ,  LOWERY, Michael, G. ,  OOSTA, Gary, M. ,  LOOMIS, Neil, W. ,  SHAIN, Eric, B. ,  SCHAPIRA, Thomas, G.

IPC: G01N33/543

CPC classification number: G01N33/5304 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/086 ,  C08G18/0866 ,  C08G18/10 ,  G01N33/54366 ,  G01N33/54393 ,  Y10S435/81 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/809 ,  Y10S436/81

Abstract: The present invention relates to analytical devices for determining the presence or amount of an analyte in a test sample. The analytical devices comprise an inlet port, a vent, a channel, and an array of structures. The structures have immobilized reagent covalently or non-covalently attached to the surface of the structures. The immobilized reagent captures analyte in the test sample where it is detected by a detection system. The present invention also provides methods and reagents for performing assays utilizing the analytical devices of the present invention. The present invention also provides methods of manufacturing the analytical devices of the present invention.

Abstract translation: 本发明涉及用于确定测试样品中分析物的存在或量的分析装置。 分析装置包括入口，通气口，通道和结构阵列。 这些结构具有共价或非共价连接到结构表面的固定化试剂。 固定化的试剂捕获测试样品中的分析物，由检测系统检测。 本发明还提供用于使用本发明的分析装置进行测定的方法和试剂。 本发明还提供了制造本发明的分析装置的方法。