公开(公告)号：WO00079277A1

公开(公告)日：2000-12-28

申请号：PCT/CA2000/000723

申请日：2000-06-15

IPC: G01N33/534 ,  C12Q1/34 ,  C12Q1/42

CPC classification number: C12Q1/42 ,  G01N2500/00 ,  Y10S436/80 ,  Y10S436/804 ,  Y10T436/16

Abstract: The present invention uses the principle that phosphomolybdate binds to hydrophobic surfaces to isolate the phosphomolybdate complex from other phosphate-containing molecules and further uses the SPA concept to bring a radiolabeled phosphomolybdate complex in close contact with a scintillant for measurement by scintillation counting. Generally, the present invention provides an assay for detecting and measuring the amount of orthophosphate (Pi) in an aqueous reaction mixture, wherein the amount of Pi released is separated from the reaction mixture by: adding a solution of molybdate to the reaction mixture to form a phosphomolybdate complex; and contacting the phosphomolybdate complex with a hydrophobic surface, wherein the surface is capable of being separated from the aqueous reaction mixture to allow measurement of the Pi. Particularly, this invention provides an assay for measuring the ATPase activity of enzymes, more particularly, the HPV E1 helicase.

Abstract translation: 本发明使用磷钼酸盐结合疏水性表面以将磷钼酸盐复合物与其它含磷酸盐分子隔离的原理，并且进一步使用SPA概念以使放射性标记的磷钼酸盐络合物与用于通过闪烁计数进行测量的闪烁体紧密接触。 通常，本发明提供了用于检测和测量水性反应混合物中正磷酸盐（Pi）的量的测定法，其中通过以下方式将释放的Pi的量与反应混合物分离：向反应混合物中加入钼酸盐溶液以形成 磷钼酸盐络合物; 并使磷钼酸盐络合物与疏水表面接触，其中表面能够与水性反应混合物分离，以允许测量Pi。 特别地，本发明提供了用于测量酶的ATPase活性的测定法，更具体地说，涉及HPV E1解旋酶。

公开(公告)号：WO00033080A2

公开(公告)日：2000-06-08

申请号：PCT/SE1999/002252

申请日：1999-12-01

IPC: G01N33/566 ,  C07D487/04 ,  G01N33/15 ,  G01N33/49 ,  G01N33/50 ,  G01N33/543 ,  G01N33/60 ,  G01N33/80

CPC classification number: G01N33/60 ,  G01N33/80 ,  G01N2333/726 ,  Y10S436/804 ,  Y10S436/815

Abstract: The invention provides a competition binding assay for detecting P2YADP receptor ligands.

Abstract translation: 本发明涉及用于检测P2YADP受体配体的竞争性结合测定法。

公开(公告)号：WO98041865A1

公开(公告)日：1998-09-24

申请号：PCT/AU1998/000176

申请日：1998-03-18

IPC: G01N33/53 ,  G01N33/564 ,  G01N33/566

CPC classification number: G01N33/564 ,  G01N2333/78 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/807

Abstract: A predictive test for rheumatoid arthritis comprises the detection of antibodies to collagen in a biological sample from a patient by contacting the biological sample with an antigen comprising the CB10 peptide of mammalian type II collagen, or an antibody-binding fragment or variant thereof, for a time and under conditions for an antibody-antigen complex to form, and detecting the antibody-antigen complex, for example by immunoassay. A diagnostic test kit is also disclosed.

Abstract translation: 对类风湿性关节炎的预测试验包括通过使生物样品与包含哺乳动物II型胶原的CB10肽或其抗体结合片段或变体的抗原接触来检测来自患者的生物样品中的胶原抗体， 时间和条件下使抗体 - 抗原复合物形成，并通过免疫测定法检测抗体 - 抗原复合物。 还公开了诊断测试试剂盒。

公开(公告)号：WO1996010747A1

公开(公告)日：1996-04-11

申请号：PCT/US1995012462

申请日：1995-09-29

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HANSMANN, Douglas, D. ,  GRACE, John, P. ,  LOWERY, Michael, G. ,  OOSTA, Gary, M. ,  LOOMIS, Neil, W. ,  SHAIN, Eric, B. ,  SCHAPIRA, Thomas, G.

IPC: G01N33/543

CPC classification number: G01N33/5304 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/086 ,  C08G18/0866 ,  C08G18/10 ,  G01N33/54366 ,  G01N33/54393 ,  Y10S435/81 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/809 ,  Y10S436/81

Abstract: The present invention relates to analytical devices for determining the presence or amount of an analyte in a test sample. The analytical devices comprise an inlet port, a vent, a channel, and an array of structures. The structures have immobilized reagent covalently or non-covalently attached to the surface of the structures. The immobilized reagent captures analyte in the test sample where it is detected by a detection system. The present invention also provides methods and reagents for performing assays utilizing the analytical devices of the present invention. The present invention also provides methods of manufacturing the analytical devices of the present invention.

Abstract translation: 本发明涉及用于确定测试样品中分析物的存在或量的分析装置。 分析装置包括入口，通气口，通道和结构阵列。 这些结构具有共价或非共价连接到结构表面的固定化试剂。 固定化的试剂捕获测试样品中的分析物，由检测系统检测。 本发明还提供用于使用本发明的分析装置进行测定的方法和试剂。 本发明还提供了制造本发明的分析装置的方法。

公开(公告)号：WO1995014039A1

公开(公告)日：1995-05-26

申请号：PCT/US1994013199

申请日：1994-11-15

Applicant: THE SCRIPPS RESEARCH INSTITUTE

Inventor： THE SCRIPPS RESEARCH INSTITUTE ,  KIRKLAND, Theo ,  TOBIAS, Peter ,  ULEVITCH, Richard ,  MORIARTY, Ann ,  LETURCQ, Didier

IPC: C07K16/18

CPC classification number: A61K49/0058 ,  A61K38/00 ,  A61K49/0043 ,  A61K51/1018 ,  C07K16/18 ,  Y10S436/804

Abstract: The present invention concerns a method of treating LBP-mediated LPS-induced myeloid cell activation comprising administering a therapeutically effective amount of an anti-LBP monoclonal antibody molecule. A therapeutic composition comprising anti-LBP antibody molecules in a pharmaceutically acceptable excipient is also contemplated.

Abstract translation: 本发明涉及治疗LBP介导的LPS诱导的骨髓细胞活化的方法，包括给予治疗有效量的抗LBP单克隆抗体分子。 还考虑了在药学上可接受的赋形剂中包含抗LBP抗体分子的治疗组合物。

公开(公告)号：WO1995010632A1

公开(公告)日：1995-04-20

申请号：PCT/US1994011746

申请日：1994-10-11

Applicant: EMORY UNIVERSITY

Inventor： EMORY UNIVERSITY ,  COMPANS, Richard, William

IPC: C12Q01/70

CPC classification number: G01N33/502 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C07K16/1027 ,  C12N2710/24143 ,  C12N2760/18022 ,  G01N33/5008 ,  G01N33/56983 ,  G01N2333/115 ,  Y10S436/804

Abstract: A method is provided for detecting whether a test composition can inhibit cell fusion mediated by paramyxovirus infection. A method is also provided for identifying an antiviral peptide capable of inhibiting paramyxovirus-induced cell fusion. The methods are based on the discovery that the hemagglutination and fusion-inducing proteins of paramyxoviruses interact to form a complex termed HN:F, that is prerequisite to cell fusion. Antibodies to HN:F provide a novel means of providing improved immunity against paramyxovirus infection.

Abstract translation: 提供了一种用于检测测试组合物是否能抑制由副粘病毒感染介导的细胞融合的方法。 还提供了一种鉴定能够抑制副粘病毒诱导的细胞融合的抗病毒肽的方法。 该方法基于以下发现：副粘病毒的血凝和融合诱导蛋白质相互作用形成称为HN：F的复合物，这是细胞融合的先决条件。 HN：F的抗体提供了一种提供针对副粘病毒感染的改善免疫的新手段。

公开(公告)号：WO1995010308A1

公开(公告)日：1995-04-20

申请号：PCT/US1994011603

申请日：1994-10-12

Applicant: DRUKIER, Andrzej, K.

IPC: A61K123/00

CPC classification number: G01T7/08 ,  G01N33/60 ,  G01T1/2008 ,  G01T1/204 ,  G01T1/208 ,  Y10S436/804 ,  Y10S436/807 ,  Y10T436/13

Abstract: For isotopes decaying by capture of an inner shell electron by the nucleus, coincident emission of X-ray and gamma photons may occur. The X-ray results from the drop of an outer shell electron to fill the S shell. The gamma results from the transition of the excited daughter nucleus to a lower energy state. The invention disclosed is a Coincident Gamma and X-ray Detector (CGXD) which achieves extraordinary background rejection by a synergistic combination of coincident counting and other background suppresion measures. Whereas the background registred by single gamma counters is of the order of 20-40 counts per minute, a CGXD optimized for the electron capture radioisotope I has a background of about one count per day.

Abstract translation: 对于通过核捕获内壳电子而衰变的同位素，可能发生X射线和γ光子的重合发射。 X射线源于外壳电子的下降以填充S壳。 伽马由激发的子核​​转变为较低能量状态。 所公开的发明是重合伽马和X射线检测器（CGXD），其通过重合计数和其他背景抑制措施的协同组合实现非凡的背景排斥。 而单个伽马计数器注册的背景数量为每分钟20-40个数量级，针对电子捕获放射性同位素I <125>优化的CGXD具有每天大约一个计数的背景。

公开(公告)号：WO9418324A3

公开(公告)日：1995-01-05

申请号：PCT/US9401368

申请日：1994-02-07

Applicant: SALK INST FOR BIOLOGICAL STUDI

Inventor： MONTMINY MARC R

IPC: G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  C07K7/06 ,  C07K7/08 ,  C07K14/47 ,  C07K16/18 ,  C12N15/12 ,  C12P21/08 ,  G01N33/577 ,  A61K37/02 ,  C07K13/00 ,  G01N33/532

CPC classification number: C07K14/4702 ,  A61K38/00 ,  C07K16/18 ,  Y10S436/804

Abstract: Phospho-specific antibodies which recognize the phosphorylated forms of cAMP-responsive transcription factors are provided.

公开(公告)号：WO1994026413A1

公开(公告)日：1994-11-24

申请号：PCT/GB1994001040

申请日：1994-05-16

Applicant: AMERSHAM INTERNATIONAL PLC ,  COOK, Neil, David

Inventor： AMERSHAM INTERNATIONAL PLC

IPC: B01L03/00

CPC classification number: B01L3/5085 ,  C12M23/12 ,  C12M23/20 ,  C12M23/22 ,  Y10S436/804

Abstract: Apparatus and method for studying cellular processes comprise a vessel having a base including a layer comprising a scintillant substance and which is adapted for attachment and/or growth of cells. Cellular processes are examined by scintillation proximity assay using a reagent labeled with a radioisotope.

Abstract translation: 用于研究细胞过程的装置和方法包括具有包括闪烁体物质并且适于细胞附着和/或生长的层的基底的容器。 通过使用用放射性同位素标记的试剂的闪烁亲近测定来检查细胞过程。

公开(公告)号：WO1993013089A1

公开(公告)日：1993-07-08

申请号：PCT/US1985000109

申请日：1985-01-22

Applicant: URBAN, Frank, J.

IPC: C07D323/00

CPC classification number: C07D405/12 ,  A61K38/00 ,  C07C43/23 ,  C07C323/16 ,  C07C323/20 ,  C07D323/00 ,  C07K14/4703 ,  C07K16/18 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S530/828 ,  Y10S530/839

Abstract: A series of novel, macrocyclic polyether compounds. The macrocycles have a 21-membered ring, containing six oxygen atoms, and they have a carboxy group (or a salt thereof) directed towards the interior of the ring. Administration of the compounds of the invention to ruminant animals (e.g. cattle and sheep) modifies their digestive fermentation processes such that the volatile fatty acids produced in the rumen contain a higher proportion of propionates rather than acetates, thereby increasing the efficiency of feed utilization in said ruminant animals. Additionally, the compounds of the invention show antibacterial activity in vitro against certain gram-positive microorganisms.

Abstract translation: 一系列新型大环聚醚化合物。 大环具有含有6个氧原子的21元环，它们具有指向环内部的羧基（或其盐）。 将本发明化合物施用于反刍动物（例如牛和绵羊）改变其消化发酵过程，使得在瘤胃中产生的挥发性脂肪酸含有较高比例的丙酸酯而不是乙酸酯，从而提高所述 反刍动物。 此外，本发明的化合物在体外对某些革兰氏阳性微生物表现出抗菌活性。