公开(公告)号：WO1996010747A1

公开(公告)日：1996-04-11

申请号：PCT/US1995012462

申请日：1995-09-29

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HANSMANN, Douglas, D. ,  GRACE, John, P. ,  LOWERY, Michael, G. ,  OOSTA, Gary, M. ,  LOOMIS, Neil, W. ,  SHAIN, Eric, B. ,  SCHAPIRA, Thomas, G.

IPC: G01N33/543

CPC classification number: G01N33/5304 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/086 ,  C08G18/0866 ,  C08G18/10 ,  G01N33/54366 ,  G01N33/54393 ,  Y10S435/81 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/809 ,  Y10S436/81

Abstract: The present invention relates to analytical devices for determining the presence or amount of an analyte in a test sample. The analytical devices comprise an inlet port, a vent, a channel, and an array of structures. The structures have immobilized reagent covalently or non-covalently attached to the surface of the structures. The immobilized reagent captures analyte in the test sample where it is detected by a detection system. The present invention also provides methods and reagents for performing assays utilizing the analytical devices of the present invention. The present invention also provides methods of manufacturing the analytical devices of the present invention.

Abstract translation: 本发明涉及用于确定测试样品中分析物的存在或量的分析装置。 分析装置包括入口，通气口，通道和结构阵列。 这些结构具有共价或非共价连接到结构表面的固定化试剂。 固定化的试剂捕获测试样品中的分析物，由检测系统检测。 本发明还提供用于使用本发明的分析装置进行测定的方法和试剂。 本发明还提供了制造本发明的分析装置的方法。

公开(公告)号：WO1995017965A1

公开(公告)日：1995-07-06

申请号：PCT/US1994014510

申请日：1994-12-16

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  BERMAN, Marvin ,  FORNEY, Kevin, J. ,  GEIST, Jill, M. ,  KORN, Jacqueline, J. ,  LANGNER, Thomas, J. ,  LOOMIS, Neil, W. ,  MORICI, Laura, S. ,  MUETTERTIES, Andrew, J. ,  PARSONS, Robert, G. ,  PUTMAN, Jill, M. ,  ROPELLA, Paul, J. ,  SCHAPIRA, Thomas, G. ,  SIEGEL, Neal, A. ,  WAGNER, Brian, K. ,  BASORE, Bob, O.

IPC: B01L03/00

CPC classification number: G01N33/521 ,  B01L3/0241 ,  B01L3/5023 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406

Abstract: The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material (20) in liquid communication with a capillary track (18). In particular, the capillary track (18) is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site (22) on or in the porous material (20). Signal development at the reaction site (22) indicates the assay result. The present invention is also directed to the construction of a disposable assay device (10) which includes a capillary track (18). In particular, the capillary track is formed by printing a fluid insoluble material (25a), in the reverse image of the desired capillary track (18), on a film layer (23) and then capping the printed material. Alternatively, the capillary track (18) is formed by printing a fluid insoluble material, in the reverse image (30) of the desired capillary track (18), on a porous material (25b) which is then sandwiched between two film layers (23, 26). The present invention is also directed to a drop-forming means (50) which may incorporate one or more assay reagents to allow for improved sample processing and reagent addition, mixing and incubation.

Abstract translation: 本发明涉及使用包括与毛细管道（18）液体连通的多孔材料（20）的一次性测定装置来改进测定的性能。 特别地，毛细管轨道（18）与固体支持物一起使用，以将测试样品和测定试剂直接引导到多孔材料（20）上或多孔材料（20）中的限定的反应位点（22）。 反应位点（22）的信号发育表明测定结果。 本发明还涉及包括毛细管轨道（18）的一次性分析装置（10）的构造。 特别地，通过在所需毛细管轨迹（18）的反向图像中将薄膜层（23）上的流体不溶性材料（25a）印刷，然后对印刷材料进行封盖来形成毛细管轨迹。 或者，毛细管轨道（18）通过在所需毛细管轨道（18）的反向图像（30）中的多孔材料（25b）上印刷流体不溶性材料而形成，然后夹在两个膜层（23 ，26）。 本发明还涉及一种液滴形成装置（50），其可以掺入一种或多种测定试剂以允许改进的样品处理和试剂添加，混合和孵育。

公开(公告)号：WO1994009366A1

公开(公告)日：1994-04-28

申请号：PCT/US1993008751

申请日：1993-09-16

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  FORNEY, Kevin, J. ,  GEIST, Jill, M. ,  LOOMIS, Neil, W. ,  MORICI, Laura, S. ,  MUETTERTIES, Andrew, J. ,  PARSONS, Robert, G. ,  PUTMAN, Jill, M. ,  ROPELLA, Paul, J. ,  SCHAPIRA, Thomas, G. ,  SIEGEL, Neal, A. ,  WAGNER, Brian, K. ,  BASORE, Bob, O.

IPC: G01N33/543

CPC classification number: B01L3/502707 ,  B01L3/5023 ,  B01L3/50273 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/0825 ,  B01L2400/0406 ,  G01N33/54366 ,  G01N33/54386

Abstract: The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material in liquid communication with a capillary track. In particular, the capillary track is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site on or in the porous material. Signal development at the reaction site indicates the assay result. The present invention is also directed to the construction of a disposable assay device which includes a capillary track. In particular, the cappillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a film layer and then capping the printed material. Alternatively, the capillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a porous material which is then sandwiched between two film layers.

Abstract translation: 本发明涉及使用包括与毛细管轨道液体连通的多孔材料的一次性测定装置来改进测定的性能。 特别地，毛细管轨道与固体支持物一起使用以将测试样品和测定试剂直接引导至多孔材料上或其中的限定的反应位点。 反应位点的信号发育表明测定结果。 本发明还涉及包括毛细管轨迹的一次性测定装置的构造。 特别地，通过在所需毛细管轨迹的反向图像中将流体不溶性材料印刷在膜层上并且然后对印刷材料进行封盖而形成毛细管轨迹。 或者，毛细管轨迹是通过在所需毛细管轨道的反向图像中印刷流体不溶性材料形成在多孔材料上，然后夹在两个膜层之间。