公开(公告)号：WO2019096903A1

公开(公告)日：2019-05-23

申请号：PCT/EP2018/081367

申请日：2018-11-15

Applicant: NOVOZYMES A/S

Inventor： HAAHR, Laerke, Tvedebrink ,  CARSTENSEN, Lone ,  ROSSO, Diego, Filipe

IPC: A23K20/189 ,  C12N9/38 ,  A23K10/14

CPC classification number: A23K20/189 ,  A23K10/14 ,  C12N9/2468 ,  C12Y302/01089

Abstract: The present invention relates to methods, uses and animal feeds employing polypeptides having galactanase activity, wherein the galactanases are used for processing soy-based material.

公开(公告)号：WO2014028774A3

公开(公告)日：2014-05-08

申请号：PCT/US2013055200

申请日：2013-08-15

Applicant: BANGLADESH JUTE RES INST

Inventor： ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL ,  ALAM MAQSUDUL

IPC: C12N9/42 ,  C12N15/00

CPC classification number: C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

Abstract: The present invention provides nucleotide sequences of Macrophomina phaseolina ("M. phaseolina") that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a &bgr;-glucosidase, a a-glucosidase, a xylanase, a mannanse, a &bgr;-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a- fucosidases, a &bgr;-galactanase, an unsaturated &bgr;-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

Abstract translation: 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

公开(公告)号：WO99003351A1

公开(公告)日：1999-01-28

申请号：PCT/DK1998/000335

申请日：1998-07-16

IPC: A21D8/04 ,  A23L7/104 ,  A23L7/109 ,  A23L1/16

CPC classification number: C12Y101/03009 ,  A21D8/042 ,  A23L7/107 ,  A23L7/109 ,  A23V2002/00 ,  C12Y302/01089 ,  A23V2250/5056 ,  A23V2250/5084 ,  A23V2250/308 ,  A23V2250/5118 ,  A23V2250/503

Abstract: A dough and bread improving composition comprising an enzyme having galactose oxidase activity and an oxidizable substrate for this enzyme and/or an enzyme which is capable of converting a compound, e.g. a galactose-containing compound into a substrate for the enzyme having galactose oxidase activity.

Abstract translation: 一种面团和面包改良组合物，其包含具有半乳糖氧化酶活性的酶和该酶的可氧化底物和/或能够转化化合物的酶，例如， 含半乳糖的化合物转化成具有半乳糖氧化酶活性的酶的底物。

公开(公告)号：WO2009146464A2

公开(公告)日：2009-12-03

申请号：PCT/US2009045883

申请日：2009-06-01

Applicant: EDENSPACE SYSTEMS CORP ,  PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

Inventor： PAPPAN KIRK ,  NAIR RAMESH ,  CHUMLEY FORREST

IPC: C12N15/55 ,  C12N9/18 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12N15/82

CPC classification number: C12N15/8245 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12P19/00 ,  C12P19/14 ,  C12Y301/01011 ,  C12Y301/01072 ,  C12Y301/01073 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/01021 ,  C12Y302/01032 ,  C12Y302/01037 ,  C12Y302/01039 ,  C12Y302/0104 ,  C12Y302/01055 ,  C12Y302/01067 ,  C12Y302/01073 ,  C12Y302/01074 ,  C12Y302/01078 ,  C12Y302/01082 ,  C12Y302/01089 ,  C12Y302/01091 ,  C12Y302/01099 ,  C12Y302/01136 ,  C12Y302/01151 ,  C12Y302/01156 ,  C12Y402/02002 ,  C12Y402/0201 ,  Y02E50/343

Abstract: Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol including compositions of matter comprising plant biomass and cell wall-modifying enzyme polypeptides and transgenic plants expression cell wall- modifying enzyme polypeptides.

Abstract translation: 改进的用于降低成本和提高纤维素乙醇产率的系统和方法，包括包含植物生物量和细胞壁修饰酶多肽的物质组合物以及转基因植物表达细胞壁修饰酶多肽。

公开(公告)号：WO01059083A1

公开(公告)日：2001-08-16

申请号：PCT/DK2001/000079

申请日：2001-02-05

IPC: A23K1/165 ,  A23L2/84 ,  C12N9/24 ,  C12N9/38

CPC classification number: C12Y302/01089 ,  A23K10/14 ,  A23K20/189 ,  A23L2/84 ,  C12N9/2468

Abstract: The invention relates to an isolated polypeptide with galactanase activity, polynucleotides encoding a galactanase, a method for modifying animal feed, in particular animal feed comprising plant material such as soybean, by adding to the animal feed at least one galactanase enzyme, and also to a method for obtaining a DNA sequence encoding a galactanase enzyme or a portion thereof.

Abstract translation: 本发明涉及具有半乳聚糖酶活性的分离的多肽，编码半乳聚糖酶的多核苷酸，通过向动物饲料中加入至少一种半乳聚糖酶而改进动物饲料的方法，特别是包含植物材料如大豆的动物饲料，以及 获得编码半乳聚糖酶或其部分的DNA序列的方法。

公开(公告)号：WO0047711A3

公开(公告)日：2000-11-30

申请号：PCT/DK0000052

申请日：2000-02-08

Applicant: NOVO NORDISK AS

Inventor： BJOERNVAD MADS ESKELUND ,  CLAUSEN IB GROTH ,  SCHUELEIN MARTIN ,  BECH LISBETH ,  OESTERGAARD PETER RAHBEK ,  SJOEHOLM CARSTEN

IPC: A23K1/165 ,  A23L2/84 ,  C11B3/00 ,  C11D3/38 ,  C11D3/386 ,  C12N9/38 ,  A23K1/14

CPC classification number: C12N9/2468 ,  A23K20/189 ,  A23L2/84 ,  C11B3/003 ,  C11D3/38 ,  C11D3/38636 ,  C12Y302/01089

Abstract: The invention relates to a method for modifying animal feed, in particular animal feed comprising plant material such as soybean, by adding to the animal feed at least one galactanase enzyme, to a method for obtaining a DNA sequence encoding a galactanase enzyme or a portion thereof, and to isolated polynucleotide molecules encoding polypeptides having galactanase activity.

Abstract translation: 本发明涉及通过向动物饲料添加至少一种半乳聚糖酶来改变动物饲料，特别是包含植物材料如大豆的动物饲料的方法，涉及获得编码半乳聚糖酶或其一部分的DNA序列的方法 并涉及编码具有半乳聚糖酶活性的多肽的分离的多核苷酸分子。

公开(公告)号：WO1992010945A1

公开(公告)日：1992-07-09

申请号：PCT/GB1991002246

申请日：1991-12-16

Applicant: ENZYMATIX LTD. ,  BIOTAL LTD. ,  EVANS, Christopher, Thomas ,  MANN, Stephen, Philip ,  CHARLEY, Robert, Clive ,  PARFITT, David

Inventor： ENZYMATIX LTD. ,  BIOTAL LTD.

IPC: A23K03/00

CPC classification number: C12Y301/01011 ,  A23K10/14 ,  A23K30/18 ,  C12Y302/01001 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/01023 ,  C12Y302/01031 ,  C12Y302/01032 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01088 ,  C12Y302/01089 ,  C12Y402/0201

Abstract: A formulation that can be used to stabilise silage from cereal and other crops, and to enhance its nutritive value and digestibility in a ruminant, is of: enzymes comprising beta -1,4-xylanase and beta -1,3-xylosidase, but substantially free of beta -1,4-glucanase and beta -1,4-cellobiohydrolase; and one or more microorganisms selected for their ability to convert pentosans to lactic acid, without degrading protein, at pH 4-6.5.

Abstract translation: 可用于稳定青贮饲料从谷物和其他作物以及增强其在反刍动物中的营养价值和消化率的制剂是：包含β-1,4-木聚糖酶和β-1,3-木糖苷酶的酶，但基本上 不含β-1,4-葡聚糖酶和β-1,4-纤维二糖水解酶; 和一种或多种微生物，其选择的能力是将戊聚糖转化为乳酸，而不降解蛋白质，pH 4-6.5。

公开(公告)号：WO2008116878A1

公开(公告)日：2008-10-02

申请号：PCT/EP2008/053561

申请日：2008-03-26

Applicant: NOVOZYMES A/S

Inventor： LASSEN, Soeren Flensted ,  SJOEHOLM, Carsten ,  SKOV, Lars Kobberoee

IPC: C12N15/55 ,  A23L1/03 ,  C12N9/16 ,  C12N15/82

CPC classification number: C12N9/16 ,  A23K20/147 ,  A23K20/174 ,  A23K20/189 ,  A23K50/00 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/18 ,  C12Y301/01004 ,  C12Y301/01005 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/04003 ,  C12Y301/04004 ,  C12Y302/01001 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01022 ,  C12Y302/01032 ,  C12Y302/01089 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.

Abstract translation: 本发明涉及具有植酸酶活性的分离的多肽和编码多肽的分离的多核苷酸。 多肽与源自Hafnia alvei的植酸酶相关，其氨基酸序列在附加的序列表中显示为SEQ ID NO：10。本发明还涉及核酸构建体，载体和宿主细胞，其包含多核苷酸为 以及用于产生和使用多肽的方法，特别是在动物饲料中。

公开(公告)号：WO2004056988A3

公开(公告)日：2004-08-12

申请号：PCT/DK0300851

申请日：2003-12-11

Applicant: NOVOZYMES AS ,  MARIA LEONARDO DE ,  SVENDSEN ALLAN ,  BORCHERT TORBEN VEDEL ,  CHRISTENSEN LARS LEHMANN HYLLI ,  LARSEN SINE ,  RYTTERSGAARD CARSTEN

Inventor： DE MARIA LEONARDO ,  SVENDSEN ALLAN ,  BORCHERT TORBEN VEDEL ,  CHRISTENSEN LARS LEHMANN HYLLI ,  LARSEN SINE ,  RYTTERSGAARD CARSTEN

IPC: C12N9/38

CPC classification number: C12N9/2468 ,  C12Y302/01089

Abstract: Variants of Glycoside hydrolase family 53 galactanases having an ateration in at least one position out of a large number of positions. The variants may be used in the dairy industry, for preparation of galacto-oligo-saccharides or for hydrolysis of lactose, and may be derived from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.

Abstract translation: 糖苷水解酶家族的53个半乳聚糖酶在大量位置上具有至少一个位置的位置。 这些变体可以用于乳制品工业，用于制备低聚半乳糖或用于水解乳糖，并且可以衍生自耶尔森氏菌，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium ，假单胞菌属，热氧化酵母或黄单胞菌属。

公开(公告)号：WO2003028476A1

公开(公告)日：2003-04-10

申请号：PCT/JP2002/009250

申请日：2002-09-11

Applicant: 明治製菓株式会社 ,  独立行政法人産業技術総合研究所 ,  西沢 耕治 ,  野尻 宙平 ,  林 淑恵 ,  深澤 朝幸 ,  山辺 倫

Inventor： 西沢 耕治 ,  野尻 宙平 ,  林 淑恵 ,  深澤 朝幸 ,  山辺 倫

IPC: A23K1/165

CPC classification number: A61K38/47 ,  A23K20/189 ,  A23K50/75 ,  C12Y302/01089

Abstract: Enzyme compositions for elevating poultry egg yield, elevating egg shell strength, extending ovipositing period and/or enriching bone density which contain an enzyme capable of forming a hardly digestible oligosaccharide from fibrous materials in the digestive tract of poultry and a carrier for the enzyme compositions; and feed preparations for elevating poultry egg yield, elevating egg shell strength, extending ovipositing period and/or enriching bone density which contain the above enzyme.

Abstract translation: 用于提高家禽蛋产量，提高蛋壳强度，延长产卵期和/或富集骨密度的酶组合物，其含有能够从家禽消化道中的纤维材料形成难消化的寡糖的酶和用于该酶组合物的载体; 提高鸡蛋产蛋率，提高蛋壳强度，延长产卵期和/或含有上述酶的骨密度的饲料制剂。