公开(公告)号：WO2013177953A1

公开(公告)日：2013-12-05

申请号：PCT/CN2013/000647

申请日：2013-05-31

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

Inventor： ZHU, Jiang ,  GUO, Hongju ,  MEI, Danyang ,  SONG, Xiumei ,  WANG, Dong ,  CHENG, Jing

IPC: C12M1/34 ,  G01N33/68

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L2400/0487

Abstract: A system for real-time monitoring of a microfluidic reaction and a method thereof are provided. The system comprises a platform for holding a microfluidic device; a temperature control module (4); a peripheral flow module; and a real-time imaging module. The method comprises providing a reagent to the microfluidic device; and monitoring the reaction using the real-time imaging module. Furthermore, methods for optimizing a reaction condition using the system are provided.

Abstract translation: 提供了一种用于实时监测微流体反应的系统及其方法。 该系统包括用于保持微流体装置的平台; 温度控制模块（4）; 外围流模块; 和实时成像模块。 该方法包括向微流体装置提供试剂; 并使用实时成像模块来监测反应。 此外，提供了使用该系统优化反应条件的方法。

公开(公告)号：WO2015149569A1

公开(公告)日：2015-10-08

申请号：PCT/CN2015/000225

申请日：2015-03-31

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

Inventor： LIU, Yan ,  ZHANG, Guohao ,  ZHU, Jiang ,  FENG, Zhengde ,  GUO, Hongju ,  GAO, Jian ,  XING, Wanli ,  CHENG, Jing

IPC: C12M1/34 ,  C12M1/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/502723 ,  B01L2200/10 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/6844

Abstract: In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.

Abstract translation: 一方面，本公开提供了用于核酸扩增和微阵列检测的集成微流体装置。 在一个方面，该装置包括：（1）微芯片，被配置为处理试剂，包括多个储存器，通道，阀和/或流体界面; （2）用于PCR的扩增室，通过接头在组装在微芯片上的可拆卸管中进行; 和（3）包含微阵列和反应室的微阵列室。 在一些实施方案中，这些特征相互连接以允许在封闭系统中运送用于核酸扩增和杂交检测功能的试剂。 在一个方面，本文的整合装置克服了扩增和杂交反应期间的污染问题。