公开(公告)号：WO2011160430A1

公开(公告)日：2011-12-29

申请号：PCT/CN2011/001013

申请日：2011-06-17

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  HAN, Chao ,  HUANG, Guoliang ,  CHENG, Jing

Inventor： HAN, Chao ,  HUANG, Guoliang ,  CHENG, Jing

IPC: A61B17/435 ,  A61B19/04

CPC classification number: A61B17/43 ,  A61B17/435 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2300/0851 ,  B01L2400/086 ,  C12M21/06 ,  C12M23/16 ,  C12M47/04

Abstract: An integrated microfluidic device and its usage are provided. The microfluidic device comprises an upper layer (1) and a lower layer (2), wherein the lower layer (2) is bound to the upper layer (1). The upper layer (1) comprises a micro-channel (3) and the lower layer (2) comprises a micro-well (7) array. The micro-channel (3) is in fluidic connection with the micro-well (7) array, and the height of the micro-channel (3) is greater than the diameter of the oocyte (4) flowing through the micro-channel (3). The integrated microfluidic device has many advantages including low cost, high integration, and convenient operation, and has application prospects in reproductive medicine and the research of fertilization and embryo early development.

Abstract translation: 提供集成的微流体装置及其用途。 微流体装置包括上层（1）和下层（2），其中下层（2）与上层（1）结合。 上层（1）包括微通道（3），下层（2）包括微孔（7）阵列。 微通道（3）与微孔（7）阵列流体连接，并且微通道（3）的高度大于流过微通道的卵母细胞（4）的直径（ 3）。 集成微流体装置具有成本低，整合性高，操作方便等优点，在生殖医学和受精胚胎早期发育研究中具有应用前景。

公开(公告)号：WO2007076688A1

公开(公告)日：2007-07-12

申请号：PCT/CN2006/003542

申请日：2006-12-22

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  GUO, Min ,  SHEN, Keyue ,  ZHOU, Cheng ,  GUAN, Xiaosheng ,  CHENG, Jing

Inventor： GUO, Min ,  SHEN, Keyue ,  ZHOU, Cheng ,  GUAN, Xiaosheng ,  CHENG, Jing

IPC: G05D23/19 ,  H05B6/06 ,  G01K7/18

CPC classification number: G01K7/18 ,  G01K7/183 ,  G05D23/2401

Abstract: Methods and devices for controlling temperature by precise heating without the need of using a temperature sensor are provided. The device comprises a resistive heating element (6), a controller (3), a heating circuit and a temperature sensing circuit. The temperature of the resistive heating element can be determined based on the resistance of the resistive heating element (6), which changes monotonically with its temperature. The resistive heating element (6) thus serves both as a heating element and as a temperature sensor, thereby obviating the need for a separate temperature sensor.

Abstract translation: 提供了通过精确加热来控制温度而不需要使用温度传感器的方法和装置。 该装置包括电阻加热元件（6），控制器（3），加热电路和温度检测电路。 电阻加热元件的温度可以基于其温度单调变化的电阻加热元件（6）的电阻来确定。 因此，电阻加热元件（6）既用作加热元件又用作温度传感器，从而避免需要单独的温度传感器。

公开(公告)号：WO2007022667A1

公开(公告)日：2007-03-01

申请号：PCT/CN2006/000014

申请日：2006-01-06

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  WANG, Dong ,  ZHANG, Jingchun ,  GAO, Wei ,  LI, Yingna ,  ZOU, Kun ,  CHENG, Jing

Inventor： WANG, Dong ,  ZHANG, Jingchun ,  GAO, Wei ,  LI, Yingna ,  ZOU, Kun ,  CHENG, Jing

IPC: B01L3/02

CPC classification number: B01L3/021 ,  B01L3/0279 ,  B01L3/0293 ,  B01L2400/0487 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/103

Abstract: An embodiment of the present invention comprises an automatic multichannel pipettor operating in coordination with a robotic positioner. A volumetrically apportioned pressure dividing manifold equally divides volumetric displacement from a syringe pump among a plurality of pipetting channels for simultaneous aspiration and dispensing. The automatic pipettor can use disposable pipette tips that can be automatically loaded and discarded.

Abstract translation: 本发明的实施例包括与机器人定位器协调操作的自动多通道移液器。 体积分配的分压歧管在多个用于同时抽吸和分配的移液通道之间等同地分配来自注射器泵的体积位移。 自动移液器可以使用可以自动加载和丢弃的一次性吸头。

公开(公告)号：WO2007014500A1

公开(公告)日：2007-02-08

申请号：PCT/CN2005/001684

申请日：2005-10-13

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  BEIJING INSTITUTE FOR CANCER RESEARCH ,  LU, Youyong ,  GUO, Ruifang ,  ZANG, Shizhu ,  ZHANG, Liang ,  REN, Yonghong ,  ZHUANG, Yonglong ,  CHENG, Jing

Inventor： LU, Youyong ,  GUO, Ruifang ,  ZANG, Shizhu ,  ZHANG, Liang ,  REN, Yonghong ,  ZHUANG, Yonglong ,  CHENG, Jing

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158

Abstract: The invention provides methods for detecting differential gene expression in intestinal gastric tissue in a mammal by comparing the expression of specific genes in an intestinal gastric tissue suspected of being cancerous with that of the corresponding adjacent intestinal gastric tissue or a normal gastric mucosa tissue. The methods can be used in diagnosing or monitoring the progression of intestinal gastric cancer and determining the levels of differentiation of intestinal gastric cancer. Systems and kits for methods of the invention are also provided.

Abstract translation: 本发明提供了通过比较疑似癌症的肠胃组织中的特定基因与相应的相邻肠胃组织或正常胃粘膜组织的特异性基因的表达来检测哺乳动物肠胃组织中差异基因表达的方法。 该方法可用于诊断或监测肠胃癌的进展和确定肠胃癌分化水平。 还提供了用于本发明方法的系统和试剂盒。

公开(公告)号：WO2005093419A1

公开(公告)日：2005-10-06

申请号：PCT/CN2005/000387

申请日：2005-03-28

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  SUN, Yimin ,  XING, Wanli ,  WANG, Guoqing ,  DU, Hongwu ,  ZHANG, Rong ,  CHEN, Yuan ,  WANG, Yan ,  CHENG, Jing

Inventor： SUN, Yimin ,  XING, Wanli ,  WANG, Guoqing ,  DU, Hongwu ,  ZHANG, Rong ,  CHEN, Yuan ,  WANG, Yan ,  CHENG, Jing

IPC: G01N33/543

CPC classification number: G01N33/543 ,  B01J2219/00387 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  C40B60/14 ,  G01N33/54366

Abstract: The present invention discloses methods for detection of small molecule compounds and its specific biochips. Biochips of the present invention comprise a solid support and carrier-linked small molecules immobilized onto the solid support. The invention also provides methods and kits for detection of small molecule compounds using the biochips of the invention.

Abstract translation: 本发明公开了小分子化合物及其特定生物芯片的检测方法。 本发明的生物芯片包含固体支持物和固定在固体支持物上的载体连接的小分子。 本发明还提供了使用本发明的生物芯片检测小分子化合物的方法和试剂盒。

公开(公告)号：WO2013166857A1

公开(公告)日：2013-11-14

申请号：PCT/CN2013/000535

申请日：2013-05-07

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

Inventor： YE, Jiaming ,  ZHANG, Kaihuan ,  XING, Wanli ,  CHENG, Jing

IPC: B01L3/00 ,  C12M1/00

CPC classification number: B01L3/502738 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084

Abstract: In one aspect, a microfluidic device for multiple reactions is provided, which comprises a reaction channel comprising multiple reaction chambers connected to a closed chamber or an elastic balloon outside of the microfluidic device, wherein a wall of the closed chamber is an elastic membrane; and a control channel comprising an elastic side wall, wherein the intersections between the side wall of the control channel with the reaction channel form multiple pneumatic microvalves. In another aspect, a method for conducting multiple reactions using the microfluidic device is provided, which comprises: a) filling the reaction chambers with a sample; and b) applying pressure to the control channel to expand the elastic side wall of the control channel, wherein the expanded elastic side wall forms a pneumatic microvalve that separates the reaction chambers.

Abstract translation: 在一个方面，提供了一种用于多重反应的微流体装置，其包括反应通道，该反应通道包括连接到微流体装置外部的封闭室或弹性气囊的多个反应室，其中封闭室的壁为弹性膜; 以及包括弹性侧壁的控制通道，其中所述控制通道的侧壁与所述反应通道之间的交叉形成多个气动微型阀。 在另一方面，提供了使用微流体装置进行多重反应的方法，其包括：a）用样品填充反应室; 以及b）向所述控制通道施加压力以膨胀所述控制通道的弹性侧壁，其中所述膨胀的弹性侧壁形成分离所述反应室的气动微型阀。

公开(公告)号：WO2012016431A1

公开(公告)日：2012-02-09

申请号：PCT/CN2011/001287

申请日：2011-08-05

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  LI, Hang ,  TENG, Huihui ,  MEI, Danyang ,  WANG, Wenjun ,  LI, Gang ,  QIN, Xiaokun ,  FENG, Zhengde ,  WANG, Dong ,  CHENG, Jing

Inventor： LI, Hang ,  TENG, Huihui ,  MEI, Danyang ,  WANG, Wenjun ,  LI, Gang ,  QIN, Xiaokun ,  FENG, Zhengde ,  WANG, Dong ,  CHENG, Jing

IPC: C12M1/34 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B25J11/00 ,  G01N21/6452 ,  G01N35/04 ,  G01N2035/00039 ,  G01N2035/00138 ,  G02B21/34

Abstract: An automatic slide loading device for microarray scanner comprises slide holders (1), a carrier device (2) and a positioning chamber (3), wherein the slide holder (1) can hold microarray slides (6) and the slide holder (1) is placed out of the scanning platform of the microarray scanner when the microarray scanner is in off work state, wherein the carrier device (2) is connected to the positioning chamber (3) and the carrier device (2) can load the slide holder (1) into the positioning chamber (3), wherein the positioning chamber (3) is placed above the scanning platform of the microarray scanner and is used to precisely locate the working surface of the microarray slides (6) in the slide holder (1).

Abstract translation: 一种用于微阵列扫描器的自动滑块装载装置，包括滑动架（1），载体装置（2）和定位室（3），其中滑动架（1）可以容纳微阵列滑块（6）和滑动架（1） 当微阵列扫描仪处于关闭状态时，放置在微阵列扫描器的扫描平台之外，其中载体装置（2）连接到定位室（3），并且载体装置（2）可以装载滑动架（ 1）进入定位室（3），其中定位室（3）放置在微阵列扫描仪的扫描平台上方，并用于精确地定位载玻片保持器（1）中的微阵列滑块（6）的工作表面， 。

公开(公告)号：WO2012003711A1

公开(公告)日：2012-01-12

申请号：PCT/CN2011/001094

申请日：2011-07-01

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  ZHANG, Guohao ,  GUO, Su ,  WANG, Can ,  WANG, Guoqing ,  LIU, Miao ,  ZHANG, Jinxiu ,  HAN, Bei ,  DENG, Tao ,  XING, Wanli ,  CHENG, Jing

Inventor： ZHANG, Guohao ,  GUO, Su ,  WANG, Can ,  WANG, Guoqing ,  LIU, Miao ,  ZHANG, Jinxiu ,  HAN, Bei ,  DENG, Tao ,  XING, Wanli ,  CHENG, Jing

IPC: C12M1/00 ,  F15C1/04

CPC classification number: F04B43/00 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/1827 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0019 ,  F16K99/0028 ,  F16K99/0034 ,  F16K99/0044 ,  F16K2099/0084 ,  Y10T137/4238

Abstract: Provided are a bubble-based microvalve and a microfluidic chip using the microvalve. Also provided are methods of using the microvalve for manipulating fluid in a microfluidic channel by changing the volume and/or location of the gas in the microvalve.

Abstract translation: 提供了一种基于气泡的微型阀和使用微型阀的微流控芯片。 还提供了通过改变微型阀中的气体的体积和/或位置来使用微型阀来控制微流体通道中的流体的方法。

公开(公告)号：WO2011014980A1

公开(公告)日：2011-02-10

申请号：PCT/CN2009/000895

申请日：2009-08-07

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERSITY ,  QIN, Wenyan ,  DONG, Peng ,  DENG, Tuo ,  MA, Cailing ,  MITHCHELSON, Keith ,  WEN, Hao ,  CHENG, Jing

Inventor： QIN, Wenyan ,  DONG, Peng ,  DENG, Tuo ,  MA, Cailing ,  MITHCHELSON, Keith ,  WEN, Hao ,  CHENG, Jing

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: The present invention provides methods and compositions for diagnosing and guidance of subsequent treatment for cervical cancer or/and cervical dysplasia and prognosis for survival of individuals having cervical cancer or/and cervical dysplasia based on levels or genetic status of certain microRNAs. The present invention also provides compositions comprising agents that decrease the levels of miRNAs and uses thereof for improvement of survival.

Abstract translation: 本发明提供了用于根据某些微小RNA的水平或遗传状态诊断和指导子宫颈癌或/和宫颈发育不良的随后治疗方法和指导，并预测患有子宫颈癌和/或宫颈发育不良的个体的存活。 本发明还提供了包含降低miRNA水平的试剂的组合物及其用于改善存活的用途。

公开(公告)号：WO2008128399A1

公开(公告)日：2008-10-30

申请号：PCT/CN2007/002894

申请日：2007-10-09

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  LI, Hang ,  YE, Jianxin ,  AI, Xingling ,  WANG, Xianhua ,  ZHANG, Liang ,  CHENG, Jing

Inventor： LI, Hang ,  YE, Jianxin ,  AI, Xingling ,  WANG, Xianhua ,  ZHANG, Liang ,  CHENG, Jing

IPC: C12M1/00 ,  C12M1/38 ,  B08B3/10 ,  B08B11/00 ,  C12Q1/68 ,  G01N33/53 ,  F16H21/18

CPC classification number: B08B3/044 ,  B01F11/0028 ,  B01J2219/00326 ,  B01J2219/00328 ,  B01J2219/00527 ,  B01J2219/00659 ,  B01L7/00 ,  B01L2300/0819 ,  B08B11/02 ,  Y10S435/809

Abstract: A device for biochip hybridization or washing is provided, which comprises a carousel (12), a translational movement controller, a revolving movement controller, and optionally a heating chamber (13). The revolving movement controller controls the revolving movement of the carousel and allows it to move in a wobbling fashion, allowing liquid movement of the hybridization or washing boxes (11) during hybridization and/or washing of biochips on the carousel. The translational movement controller brings the carousel back to horizontal position once the revolving movement controller stops, thereby ensures that the liquid do not spill out. The heating chamber circulates hot air within the device, thereby ensures that the hybridization and washing in a thermostatic condition.

Abstract translation: 提供了用于生物芯片杂交或洗涤的装置，其包括圆盘传送带（12），平移运动控制器，旋转运动控制器和可选的加热室（13）。 旋转运动控制器控制圆盘传送带的旋转运动，并允许其以摆动的方式移动，允许杂交或洗涤盒（11）在传送带上的生物芯片的杂交和/或洗涤期间的液体移动。 一旦旋转运动控制器停止，平移运动控制器将转盘回到水平位置，从而确保液体不会溢出。 加热室在装置内循环热空气，从而确保在恒温条件下进行杂交和洗涤。