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    A METHOD AND APPARATUS FOR SPATIALLY CONFINED ELECTROPORATION
    4.
    发明申请
    A METHOD AND APPARATUS FOR SPATIALLY CONFINED ELECTROPORATION 审中-公开
    空间约束电穿孔的方法和装置

    公开(公告)号：WO2007039783A3

    公开(公告)日：2007-08-16

    申请号：PCT/IB2004004477

    申请日：2004-11-24

    Applicant: CELLECTRICON AB ,  ORWAR OWE ,  RYTTSEN FRIDA ,  LEVIN MIKAEL ,  SAHLIN ESKIL ,  WIGSTROEM JOAKIM

    Inventor： ORWAR OWE ,  RYTTSEN FRIDA ,  LEVIN MIKAEL ,  SAHLIN ESKIL ,  WIGSTROEM JOAKIM

    IPC: A61N1/30 ,  A61B5/04 ,  A61N1/32 ,  C12N13/00 ,  G01N33/543

    CPC classification number: G01N33/5438 ,  A61N1/306 ,  C12M35/02 ,  C12N13/00 ,  G01N33/54366 ,  G01N33/54373

    Abstract: The invention provides hollow-tip-electrodes for spatially localized delivery of substances to one or more biological targets present in a population comprising target and non-target molecules, macromolecules, and/or cells. The invention also provides electrode plates for receiving one or more of such tips, tip-electrode plates comprising electrode plates comprising one or more electrode tips, and systems comprising tip-electrodes and containers for containing one or more biological targets, e.g., such as molecules, macromolecules, and/or cells. The invention further provides methods for using such systems and components thereof. In one preferred aspect, the systems are used for spatially confined electroporation of cells and cells and cells structures. The invention facilitates high throughput screening of agents (e.g., such as drugs) that act on intracellular targets.

    Abstract translation: 本发明提供了空心尖端电极，用于将物质空间局部递送至存在于包含靶分子，非靶分子，大分子和/或细胞的群体中的一种或多种生物靶标。 本发明还提供用于接收一个或多个这种尖端的电极板，包括包含一个或多个电极尖端的电极板的尖端电极板，以及包括尖端电极和用于容纳一个或多个生物靶的容器的系统，例如分子 ，大分子和/或细胞。 本发明还提供了使用这种系统及其部件的方法。 在一个优选的方面，系统用于细胞和细胞以及细胞结构的空间限定的电穿孔。 本发明促进了对作用于细胞内靶标的药剂（例如药物）的高通量筛选。

    MICROFLUIDIC DEVICES WITH HOLDING INTERFACE AND METHODS OF USE
    6.
    发明申请
    MICROFLUIDIC DEVICES WITH HOLDING INTERFACE AND METHODS OF USE 审中-公开
    具有接口的微流体装置和使用方法

    公开(公告)号：WO2012153192A8

    公开(公告)日：2013-06-20

    申请号：PCT/IB2012001047

    申请日：2012-05-07

    Applicant: ORWAR OWE ,  AINLA ALAR ,  JESORKA ALDO ,  JEFFRIES GAVIN

    Inventor： ORWAR OWE ,  AINLA ALAR ,  JESORKA ALDO ,  JEFFRIES GAVIN

    IPC: B01L3/02 ,  B01L3/00 ,  G01N33/483

    CPC classification number: G01N35/1072 ,  B01L3/021 ,  B01L3/022 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0861 ,  B01L2400/0487 ,  Y10T436/25

    Abstract: Aspects of the present invention provides a freestanding microfluidic pipette with integrated wells for solution storage. Further aspects of the invention provide a holding interface to provide connectivity with external control components. One aspect of the invention provides a system for applying a microfluidic device in microscopy. The system includes: a microfluidic device having an elongated shape and defining one or more wells for solution storage and processing; and an interface adapted and configured to hold the microfluidic device in a freestanding manner and facilitate simultaneous connection of the one or more wells with a flow controller. Another aspect of the invention provides a method for utilizing a microfluidic device. The method includes: providing a device as described herein; positioning the device adjacent to a microscope; and actuating the interface to operate the microfluidic device.

    Abstract translation: 本发明的方面提供了一种具有用于溶液储存的综合井的独立的微流体移液管。 本发明的另外方面提供了一种保持接口以提供与外部控制部件的连接。 本发明的一个方面提供一种用于在显微镜中应用微流体装置的系统。 该系统包括：具有细长形状并限定用于溶液储存和加工的一个或多个孔的微流体装置; 以及适于和配置为以独立方式保持微流体装置并且便于一个或多个井与流量控制器的同时连接的界面。 本发明的另一方面提供了一种利用微流体装置的方法。 该方法包括：提供如本文所述的装置; 将设备定位在显微镜附近; 并致动界面以操作微流体装置。

    SCREENING OF BINDERS ON IMMOBILIZED NATIVE MEMBRANE PROTEINS
    7.
    发明申请
    SCREENING OF BINDERS ON IMMOBILIZED NATIVE MEMBRANE PROTEINS 审中-公开
    固定化膜蛋白结合蛋白的筛选

    公开(公告)号：WO2011040876A9

    公开(公告)日：2011-06-30

    申请号：PCT/SE2010051052

    申请日：2010-09-30

    Applicant: NANOXIS AB ,  KARLSSON ANDERS ,  KARLSSON ROGER ,  OLESEN KENNETH ,  ORWAR OWE

    Inventor： KARLSSON ANDERS ,  KARLSSON ROGER ,  OLESEN KENNETH ,  ORWAR OWE

    IPC: G01N33/543 ,  B01D15/38 ,  B01L3/00 ,  C07K1/22 ,  C12N11/14 ,  G01N33/53

    CPC classification number: G01N33/54366 ,  B01D15/3804 ,  B01D15/428 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/0636 ,  B01L2300/0877 ,  C12N11/14

    Abstract: The present invention relates to a method for characterizing the binding interaction of a candidate binder to a target molecule comprising - providing a microfluidic flow cell having - an inlet; - at least one supporting solid surface with at least one membraneophilic region; - a covering layer that is at least partially immobilized to the membraneophilic region, said covering layer consisting of (i) a surfactant membrane, (ii) a lipid mimicking polymer, (iii) a surfactant or emulsion system, or (iv) a liquid crystal, or a combination thereof; - the target molecule included in or bound to, connected to or associated with the covering layer; and -an outlet; said method further comprising - contacting the target molecule with the candidate binders by creating an eluent flow, comprising the candidate binders, from said inlet to said outlet; - detecting and/or identifying said candidate binders in the eluate - characterizing the binding interaction of the candidate binder to the target molecule based on the detection and/or identification of the candidate binder in the eluate. The invention also relates to a device adapted for use in said method.

    Abstract translation: 本发明涉及用于表征候选结合剂与靶分子的结合相互作用的方法，包括 - 提供微流体流动池，其具有：入口; - 至少一个具有至少一个嗜热区域的支撑固体表面; - 覆盖层，其至少部分固定在所述嗜热性区域上，所述覆盖层由（i）表面活性剂膜，（ii）类脂模拟聚合物，（iii）表面活性剂或乳液体系，或（iv）液体 晶体或其组合; - 包含于或结合至，连接至或与覆盖层相关联的目标分子; 和 - 出口; 所述方法进一步包括 - 通过从所述入口到所述出口形成包含候选粘合剂的洗脱液流，使目标分子与候选粘合剂接触; - 在洗脱物中检测和/或鉴定所述候选结合物 - 基于洗脱液中候选结合物的检测和/或鉴定来表征候选结合物与目标分子的结合相互作用。 本发明还涉及一种适用于所述方法的装置。

    MICROFLUIDIC DEVICE WITH HOLDING INTERFACE, AND METHOD OF USE
    8.
    发明申请
    MICROFLUIDIC DEVICE WITH HOLDING INTERFACE, AND METHOD OF USE 审中-公开
    具有保持界面的微流体装置及其使用方法

    公开(公告)号：WO2012153192A3

    公开(公告)日：2013-03-28

    申请号：PCT/IB2012001047

    申请日：2012-05-07

    Applicant: ORWAR OWE ,  AINLA ALAR ,  JESORKA ALDO ,  JEFFRIES GAVIN

    Inventor： ORWAR OWE ,  AINLA ALAR ,  JESORKA ALDO ,  JEFFRIES GAVIN

    IPC: B01L3/02 ,  B01L3/00 ,  G01N33/483

    CPC classification number: G01N35/1072 ,  B01L3/021 ,  B01L3/022 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0861 ,  B01L2400/0487 ,  Y10T436/25

    Abstract: Aspects of the present invention provides a freestanding microfluidic pipette with integrated wells for solution storage. Further aspects of the invention provide a holding interface to provide connectivity with external control components. One aspect of the invention provides a system for applying a microfluidic device in microscopy. The system includes: a microfluidic device having an elongated shape and defining one or more wells for solution storage and processing; and an interface adapted and configured to hold the microfluidic device in a freestanding manner and facilitate simultaneous connection of the one or more wells with a flow controller. Another aspect of the invention provides a method for utilizing a microfluidic device. The method includes: providing a device as described herein; positioning the device adjacent to a microscope; and actuating the interface to operate the microfluidic device.

    Abstract translation: 本发明的方面提供了一种具有用于溶液储存的综合井的独立的微流体移液管。 本发明的另外方面提供了一种保持接口以提供与外部控制部件的连接。 本发明的一个方面提供一种用于在显微镜中应用微流体装置的系统。 该系统包括：具有细长形状并限定用于溶液储存和加工的一个或多个孔的微流体装置; 以及适于和配置为以独立方式保持微流体装置并且便于一个或多个井与流量控制器的同时连接的界面。 本发明的另一方面提供了一种利用微流体装置的方法。 该方法包括：提供如本文所述的装置; 将设备定位在显微镜附近; 并致动界面以操作微流体装置。

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