公开(公告)号：WO2018067836A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/055369

申请日：2017-10-05

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： VODYANYK, Maksym, A. ,  ZHANG, Xin ,  BRANDL, Andrew, J. ,  RAJESH, Deepika ,  SWANSON, Bradley ,  MUNN, Christie ,  BURTON, Sarah ,  WANG, Wen, Bo

IPC: C12N5/0789 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/074

Abstract: Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Abstract translation: 本文提供了用于将HLA纯合血细胞衍生的多能干细胞有效体外分化为造血前体细胞和将造血前体细胞进一步分化成各种髓样的HLA纯合免疫细胞的方法 或淋巴谱系，特别是T细胞，NK细胞和树突细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中，不需要使用小鼠饲养细胞或血清来分化造血前体细胞。

公开(公告)号：WO2017070337A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057899

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： VODYANYK, Maksym, A. ,  ZHANG, Xin ,  BRANDL, Andrew, J. ,  RAJESH, Deepika ,  SWANSON, Bradley ,  MUNN, Christie ,  BURTON, Sarah, A. ,  WANG, Wen, Bo

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Abstract translation: 本文提供了用于将体细胞衍生的多能干细胞有效体外分化为造血前体细胞以及将造血前体细胞进一步分化成各种骨髓或淋巴谱系的免疫细胞的方法， 特别是T细胞，NK细胞和树突细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中，不需要使用小鼠饲养细胞或血清来分化造血前体细胞。

公开(公告)号：WO2018067826A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/055353

申请日：2017-10-05

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： RAJESH, Deepika ,  STROUSE, Anne ,  BURTON, Sarah ,  MUNN, Christie ,  SWANSON, Bradley

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85

Abstract: Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.

Abstract translation: 本文提供了用于将MeCP2基因破坏成多种红细胞，骨髓，淋巴或内胚层谱系，特别是成熟的多能细胞的有效体外维持，扩增，培养和/或分化的方法 红细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中不需要使用小鼠饲养细胞或血清来分化前体细胞。