公开(公告)号：WO2017070333A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057893

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  VODYANYK, Maksym, A. ,  SASAKI, Jeffrey ,  RAJESH, Deepika ,  BURTON, Sarah, A.

IPC: C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: The present disclosure generally regards methods and compositions for providing multi-lineage hematopoietic precursor cells from pluripotent stem cells (PSCs). The PSCs comprise an expression construct encoding an ETS/ERG gene, GATA2 and HOXA9. Also provided are methods for providing hematopoietic stem cells capable of long-term engraftment in mammals, such as humans. Further provided are therapeutic compositions including the provided hematopoietic stem cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects

Abstract translation: 本公开一般涉及用于从多能干细胞（PSC）提供多谱系造血前体细胞的方法和组合物。 PSC包含编码ETS / ERG基因，GATA2和HOXA9的表达构建体。 还提供了用于提供能够在哺乳动物（例如人）中长期植入的造血干细胞的方法。 进一步提供了治疗性组合物，包括所提供的造血干细胞和造血细胞的前体，以及用于治疗受试者的方法

公开(公告)号：WO2017070337A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057899

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： VODYANYK, Maksym, A. ,  ZHANG, Xin ,  BRANDL, Andrew, J. ,  RAJESH, Deepika ,  SWANSON, Bradley ,  MUNN, Christie ,  BURTON, Sarah, A. ,  WANG, Wen, Bo

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Abstract translation: 本文提供了用于将体细胞衍生的多能干细胞有效体外分化为造血前体细胞以及将造血前体细胞进一步分化成各种骨髓或淋巴谱系的免疫细胞的方法， 特别是T细胞，NK细胞和树突细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中，不需要使用小鼠饲养细胞或血清来分化造血前体细胞。