公开(公告)号：WO2018067836A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/055369

申请日：2017-10-05

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： VODYANYK, Maksym, A. ,  ZHANG, Xin ,  BRANDL, Andrew, J. ,  RAJESH, Deepika ,  SWANSON, Bradley ,  MUNN, Christie ,  BURTON, Sarah ,  WANG, Wen, Bo

IPC: C12N5/0789 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/074

Abstract: Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Abstract translation: 本文提供了用于将HLA纯合血细胞衍生的多能干细胞有效体外分化为造血前体细胞和将造血前体细胞进一步分化成各种髓样的HLA纯合免疫细胞的方法 或淋巴谱系，特别是T细胞，NK细胞和树突细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中，不需要使用小鼠饲养细胞或血清来分化造血前体细胞。

公开(公告)号：WO2012018933A2

公开(公告)日：2012-02-09

申请号：PCT/US2011/046452

申请日：2011-08-03

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  THOMSON, James ,  RAJESH, Deepika ,  DICKERSON, Sarah, Jane ,  MACK, Amanda ,  MILLER, Michael

Inventor： THOMSON, James ,  RAJESH, Deepika ,  DICKERSON, Sarah, Jane ,  MACK, Amanda ,  MILLER, Michael

IPC: C12N5/074 ,  C12N5/0781 ,  C12N5/10 ,  C12N15/33

CPC classification number: C12N5/0696 ,  C12N15/63 ,  C12N15/85 ,  C12N2501/06 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/235 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/11 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2710/16243 ,  C12N2800/108

Abstract: Methods and composition for providing induced pluripotent stem (iPS) cells are provided. For example, in certain aspects methods including reprogramming B lymphocytes transformed by episomal vectors such as Epstein-Barr virus-based vectors are described. Furthermore, the invention provides induced pluripotent stem cells essentially free of exogenous elements and having B cell immunoglobin variable region rearrangement.

Abstract translation: 提供了用于提供诱导多能干（iPS）细胞的方法和组合物。 例如，在某些方面，描述了包括重编程B淋巴细胞的方法，所述B淋巴细胞由附加型载体如基于Epstein-Barr病毒的载体转化。 此外，本发明提供诱导的多能干细胞，其基本上不含外源元件并具有B细胞免疫球蛋白可变区重排。

公开(公告)号：WO2010099539A1

公开(公告)日：2010-09-02

申请号：PCT/US2010/025776

申请日：2010-03-01

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  RAJESH, Deepika ,  LEWIS, Rachel

Inventor： RAJESH, Deepika ,  LEWIS, Rachel

IPC: C12N5/0781 ,  C12N5/071

CPC classification number: C12N5/0647 ,  C12N5/0692 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2506/03 ,  C12N2506/45

Abstract: Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages.

Abstract translation: 本文提供了将多能细胞如人胚胎干细胞（hESC）或诱导的多能细胞（iPSC）体外维持，扩增，培养和/或分化到造血前体细胞或内皮细胞中的方法。 可以在限定的条件下维持和分化多能细胞; 因此，在某些实施方案中，为了将多能细胞分化成造血前体细胞或内皮细胞，不需要使用小鼠饲养细胞或血清。 所得到的造血前体细胞可进一步分化成各种骨髓或淋巴系。

公开(公告)号：WO2017070337A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057899

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： VODYANYK, Maksym, A. ,  ZHANG, Xin ,  BRANDL, Andrew, J. ,  RAJESH, Deepika ,  SWANSON, Bradley ,  MUNN, Christie ,  BURTON, Sarah, A. ,  WANG, Wen, Bo

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

Abstract translation: 本文提供了用于将体细胞衍生的多能干细胞有效体外分化为造血前体细胞以及将造血前体细胞进一步分化成各种骨髓或淋巴谱系的免疫细胞的方法， 特别是T细胞，NK细胞和树突细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中，不需要使用小鼠饲养细胞或血清来分化造血前体细胞。

公开(公告)号：WO2018067826A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/055353

申请日：2017-10-05

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： RAJESH, Deepika ,  STROUSE, Anne ,  BURTON, Sarah ,  MUNN, Christie ,  SWANSON, Bradley

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85

Abstract: Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.

Abstract translation: 本文提供了用于将MeCP2基因破坏成多种红细胞，骨髓，淋巴或内胚层谱系，特别是成熟的多能细胞的有效体外维持，扩增，培养和/或分化的方法 红细胞。 多能细胞可以在确定的条件下维持和分化; 因此，在某些实施方案中不需要使用小鼠饲养细胞或血清来分化前体细胞。

公开(公告)号：WO2017070333A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057893

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  VODYANYK, Maksym, A. ,  SASAKI, Jeffrey ,  RAJESH, Deepika ,  BURTON, Sarah, A.

IPC: C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: The present disclosure generally regards methods and compositions for providing multi-lineage hematopoietic precursor cells from pluripotent stem cells (PSCs). The PSCs comprise an expression construct encoding an ETS/ERG gene, GATA2 and HOXA9. Also provided are methods for providing hematopoietic stem cells capable of long-term engraftment in mammals, such as humans. Further provided are therapeutic compositions including the provided hematopoietic stem cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects

Abstract translation: 本公开一般涉及用于从多能干细胞（PSC）提供多谱系造血前体细胞的方法和组合物。 PSC包含编码ETS / ERG基因，GATA2和HOXA9的表达构建体。 还提供了用于提供能够在哺乳动物（例如人）中长期植入的造血干细胞的方法。 进一步提供了治疗性组合物，包括所提供的造血干细胞和造血细胞的前体，以及用于治疗受试者的方法

公开(公告)号：WO2014165663A1

公开(公告)日：2014-10-09

申请号：PCT/US2014/032804

申请日：2014-04-03

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： RAJESH, Deepika ,  BURTON, Sarah, Alice

IPC: C12N5/074 ,  C12N5/071

CPC classification number: C12N5/0676 ,  C12N5/0607 ,  C12N5/0696 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/999 ,  C12N2506/45

Abstract: Provided herein are methods for the in vitro differentiation of induced pluripotent stem cells, which have been expanded and/or maintained under defined conditions, into endodermal precursor cells (EPCs) that are capable of producing mono- hormonal beta cells.

Abstract translation: 本文提供了将已经在限定条件下扩增和/或维持的诱导多能干细胞体外分化为能够产生单一激素β细胞的内胚层前体细胞（EPCs）的方法。

公开(公告)号：WO2010141801A2

公开(公告)日：2010-12-09

申请号：PCT/US2010/037376

申请日：2010-06-04

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  BROWN, Matthew ,  DOMINGUEZ, Elizabeth, Rondon ,  LEARISH, Randy ,  NUWAYSIR, Emile ,  RAJESH, Deepika ,  MACK, Amanda

Inventor： BROWN, Matthew ,  DOMINGUEZ, Elizabeth, Rondon ,  LEARISH, Randy ,  NUWAYSIR, Emile ,  RAJESH, Deepika ,  MACK, Amanda

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0637 ,  C12N15/85 ,  C12N2501/2302 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/1114 ,  C12N2506/11 ,  C12N2510/00 ,  C12N2800/108

Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from CD34 + hematopoietic cells, such as human CD34 + blood progenitor cells, or T cells. Various iPS cell lines are also provided. In certain embodiments, the invention provides novel induced pluripotent stem cells with a genome comprising genetic rearrangement of T cell receptors.

Abstract translation: 公开了与诱导性多能干细胞（iPS细胞）的产生有关的方法和组合物。 例如，可以从CD34 +造血细胞例如人CD34 +血液祖细胞或T细胞产生诱导的多能干细胞。 还提供了各种iPS细胞系。 在某些实施方案中，本发明提供具有包含T细胞受体的遗传重排的基因组的新型诱导多能干细胞。