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公开(公告)号：WO2015094714A1
公开(公告)日：2015-06-25
申请号：PCT/US2014/068763
申请日：2014-12-05
Applicant: DANISCO US INC.
Inventor： BLAKE, Collette Michelle , CHIN, Regina , GU, Xiaogang , SHARMA, Vivek , TEUNISSEN, Paula Johanna Maria
CPC classification number: C12P19/02 , C12P7/10 , C12P19/14 , C12P2203/00 , C12Y304/00 , Y02E50/16
Abstract: The present invention relates to reducing the viscosity of grain slurry and reduction in foaming during liquefaction and/or fermentation using proteases. The invention also relates to increasing the rate and/or yield of fermentation products, including ethanol from grains.
Abstract translation: 本发明涉及在使用蛋白酶的液化和/或发酵过程中降低谷物浆料的粘度和减少发泡。 本发明还涉及增加发酵产物（包括来自谷物的乙醇）的速率和/或产率。
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2.发明申请PSEUDOMONAS SACCHAROPHILA G4-AMYLASE VARIANTS AND USES THEREOF 审中-公开PSEUDOMONAS SACCHAROPHILA G4-氨基酸变体及其用途
公开(公告)号：WO2009083592A1
公开(公告)日：2009-07-09
申请号：PCT/EP2008/068375
申请日：2008-12-30
Applicant: DANISCO A/S , Danisco US Inc., Genencor Division , KRAGH, Karsten, Matthias , KELLET-SMITH, Anja Hemmingsen , SHAW, Andrew , CHIN, Regina
CPC classification number: C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
Abstract: Pseudomonas saccharophila G4-forming amylase (PS4) variants, and nucleic acids encoding these, and their uses in producing food products and in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch are provided.
Abstract translation: 提供了糖酵母（Saccharophila）G4形成淀粉酶（PS4）变体，以及编码它们的核酸，以及它们在食品生产中的用途和在酶催化的高温液化步骤中从淀粉例如玉米淀粉生产乙醇的用途。
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Patent Agency Ranking
公开(公告)号：WO2015066667A1
公开(公告)日：2015-05-07
申请号：PCT/US2014/063791
申请日：2014-11-04
Applicant: DANISCO US INC.
Inventor： SHARMA, Vivek , TEUNISSEN, Paula Johanna Maria , CHIN, Regina
Abstract: The present invention relates to reducing the viscosity of wheat-like grain slurry during a liquefaction and/or fermentation. The invention also relates to increasing the rate and/or yield of fermentation products, including ethanol from wheat-like grains.
Abstract translation: 本发明涉及在液化和/或发酵过程中降低小麦籽粒浆料的粘度。 本发明还涉及增加发酵产物的速率和/或产率，包括来自小麦状颗粒的乙醇。
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公开(公告)号：WO2009149419A3
公开(公告)日：2009-12-10
申请号：PCT/US2009/046506
申请日：2009-06-05
Applicant: DANISCO US INC. , CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Inventor： CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Abstract: Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
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公开(公告)号：WO2015066669A1
公开(公告)日：2015-05-07
申请号：PCT/US2014/063798
申请日：2014-11-04
Applicant: DANISCO US INC.
Inventor： SHARMA, Vivek , TEUNISSEN, Paula Johanna Maria , CHIN, Regina , BLAKE, Collette Michelle , LEE, Sang-Kyu , QIAN, Ying , LIU, Xuan , DUAN, Gang , XU, Hongxian
Abstract: The present invention relates to reducing the viscosity of a corn-like grain slurry during a liquefaction and/or fermentation. The invention also relates to increasing the rate and/or yield of fermentation products, including, but not limited to ethanol, lactic acid and citric acid from corn-like grains.
Abstract translation: 本发明涉及在液化和/或发酵期间降低玉米样颗粒浆料的粘度。 本发明还涉及增加发酵产物的速率和/或产率，包括但不限于来自玉米状颗粒的乙醇，乳酸和柠檬酸。
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公开(公告)号：WO2009149271A3
公开(公告)日：2009-12-10
申请号：PCT/US2009/046279
申请日：2009-06-04
Applicant: DANISCO US INC. , CASCAO-PEREIRA, Luis, G. , CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Inventor： CASCAO-PEREIRA, Luis, G. , CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Abstract: An alpha amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
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7.发明申请
公开(公告)号：WO2009088465A3
公开(公告)日：2009-07-16
申请号：PCT/US2008/014105
申请日：2008-12-30
Applicant: DANISCO US INC., GENENCOR DIVISION , SHAW, Andrew , CHIN, Regina , KRAGH, Karsten Matthias , KELLET-SMITH, Anja Hemmingsen
Inventor： SHAW, Andrew , CHIN, Regina , KRAGH, Karsten Matthias , KELLET-SMITH, Anja Hemmingsen
Abstract: Pseudomonas saccharophila G4 forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.
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