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公开(公告)号：WO2020114893A1
公开(公告)日：2020-06-11
申请号：PCT/EP2019/083018
申请日：2019-11-29
申请人： DSM IP ASSETS B.V.
IPC分类号： C12N15/113 , C12N15/85 , C12N15/10
摘要： The invention relates to the field of molecular biology and cell biology. More specifically, the invention relates to CRISPR guide-RNA expression strategies for multiplex genome engineering.
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公开(公告)号：WO2017037304A2
公开(公告)日：2017-03-09
申请号：PCT/EP2016/081240
申请日：2016-12-15
申请人： DSM IP ASSETS B.V.
IPC分类号： C12N15/11
CPC分类号： C12N15/102 , C12N15/111 , C12N2310/20 , C12Q2521/301
摘要： The present invention is based on the adavantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
摘要翻译： 本发明基于在双链寡核苷酸体内（在细胞内）装配单链寡核苷酸单链双链核酸构建体中的有益用途。 本发明涉及在至少两个双链核酸分子的细胞内装配至预定序列的单个双链核酸构建体中的至少第一和第二单链寡核苷酸的用途， 其中所述第一和第二单链寡核苷酸彼此基本互补。
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公开(公告)号：WO2019012162A2
公开(公告)日：2019-01-17
申请号：PCT/EP2018/081942
申请日：2018-11-20
申请人： DSM IP ASSETS B.V.
CPC分类号： C12N15/102 , C12N15/81
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a genome editing system.
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公开(公告)号：WO2018127611A1
公开(公告)日：2018-07-12
申请号：PCT/EP2018/058612
申请日：2018-04-04
申请人： DSM IP ASSETS B.V.
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
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公开(公告)号：WO2020141168A1
公开(公告)日：2020-07-09
申请号：PCT/EP2019/087183
申请日：2019-12-30
申请人： DSM IP ASSETS B.V.
发明人： HOUSTON, Peter, Louis , DE JONG, René, Marcel , VERWAAL, René , VYAS, Valmik, Kanubhai , MCMAHON, Jenna , SYMBOR-NAGRABSKA, Anna
摘要： The present invention is related to production of retinyl acetate generated via enzymatic conversion of retinol, said process including the use of modified enzymes with improved activity.
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公开(公告)号：WO2019012162A3
公开(公告)日：2019-01-17
申请号：PCT/EP2018/081942
申请日：2018-11-20
申请人： DSM IP ASSETS B.V.
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a genome editing system.
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公开(公告)号：WO2017109091A1
公开(公告)日：2017-06-29
申请号：PCT/EP2016/082415
申请日：2016-12-22
申请人： DSM IP ASSETS B.V.
CPC分类号： C12Y106/05009 , C12N9/0006 , C12N9/0008 , C12N9/0036 , C12P7/46 , C12Y101/01008
摘要： The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
摘要翻译： 本发明涉及能够产生二羧酸并且在其基因组中包含至少一个遗传修饰的宿主细胞，导致缺乏至少一个催化氧化a 辅因子。 本发明还涉及生产二羧酸的方法，该方法包括在合适的发酵培养基中发酵这种宿主细胞并生产二羧酸。
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公开(公告)号：WO2016110511A1
公开(公告)日：2016-07-14
申请号：PCT/EP2016/050135
申请日：2016-01-06
申请人： DSM IP ASSETS B.V.
发明人： MEIJRINK, Bernard , VERWAAL, René , GIELESEN, Bianca Elisabeth Maria , ROUBOS, Johannes Andries
CPC分类号： C12N15/905 , C12N9/22 , C12N15/11 , C12N15/63 , C12N2310/20
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
摘要翻译： 本发明涉及分子生物学和细胞生物学领域。 更具体地，本发明涉及用于脂解酵母宿主细胞的CRISPR-CAS系统。
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公开(公告)号：WO2015007902A1
公开(公告)日：2015-01-22
申请号：PCT/EP2014/065552
申请日：2014-07-18
发明人： JANSEN, Mickel Leonardus August , VERWAAL, René , SEGUEILHA, Laurent , LOUCHART, Mélanie , VEIGA DOS INOCENTES, Tania
摘要： The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
摘要翻译： 本发明涉及二羧酸的方法，该方法包括在包含合适的发酵培养基的容器中发酵真菌细胞，其中至少一部分真菌细胞在维生素和/或微量元素存在下重复使用 。
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公开(公告)号：WO2016110512A1
公开(公告)日：2016-07-14
申请号：PCT/EP2016/050136
申请日：2016-01-06
申请人： DSM IP ASSETS B.V.
发明人： VERWAAL, René , MEIJRINK, Bernard , WIESSENHAAN, Nathalie , VONK, Brenda , ROUBOS, Johannes Andries
IPC分类号： C12N15/90
CPC分类号： C12N15/905 , C12N9/22 , C12N15/102 , C12N15/113 , C12N15/81 , C12N15/902 , C12N2310/20 , C12P23/00
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
摘要翻译： 本发明涉及分子生物学和细胞生物学领域。 更具体地，本发明涉及用于酵母宿主细胞的CRISPR-CAS系统。
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