公开(公告)号：WO2013078347A3

公开(公告)日：2013-08-01

申请号：PCT/US2012066303

申请日：2012-11-21

Applicant: HARVARD COLLEGE

Inventor： COHEN ADAM E ,  MACLAURIN DOUGAL ,  HOCHBAUM DANIEL ,  KRALJ JOEL

IPC: G02B21/00

CPC classification number: G02B21/367 ,  G01N21/6486 ,  G01N2201/06113 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/025 ,  G02B21/082 ,  G02B21/16 ,  G02B21/361

Abstract: Various aspects of the present invention are generally directed to systems and methods for imaging at high spatial and/or temporal resolutions. In one aspect, the present invention is generally directed to an optical microscopy system and related methods adapted for high spatial and temporal resolution of dynamic processes. The system may be used in conjunction with fluorescence imaging wherein the fluorescence may be mediated by voltage-indicating proteins. In some cases, time resolutions may be enhanced by fitting predefined temporal waveforms to signal values received from an image. The system may also contain a high numerical aperture objective lens and a zoom lens located in an imaging optical path to an object region. Other aspects of the present invention are generally directed to techniques of making or using such systems, kits involving such systems, manufactured storage devices able to implement such systems or methods, and the like.

Abstract translation: 本发明的各个方面总体上针对用于以高空间和/或时间分辨率进行成像的系统和方法。 在一个方面，本发明总体上针对适用于动态过程的高空间和时间分辨率的光学显微镜系统和相关方法。 该系统可以与荧光成像结合使用，其中荧光可以由电压指示蛋白介导。 在某些情况下，时间分辨率可以通过将预定义时间波形拟合到从图像接收的信号值来增强。 该系统还可以包含位于到目标区域的成像光路中的高数值孔径物镜和变焦透镜。 本发明的其他方面通常涉及制造或使用这种系统的技术，涉及这种系统的套件，能够实现这样的系统或方法的制造的存储设备等。