公开(公告)号：WO2013049702A3

公开(公告)日：2013-10-10

申请号：PCT/US2012058096

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： FREUDENTHAL JACOB K ,  DAHLHOFF GEOFFREY ,  MAHER KEVIN ,  CHEN MINGSONG ,  LIM GARY ,  DANEHY RONALD ,  FORTESCUE DAVID ,  SHEN MING ,  SOH WOON LIANG TERENCE ,  CHENG FRANCIS ,  WU RUOYUN ,  LEE THIAM CHYE ,  CHAPPELL GARY ,  LIM CHEE KIONG ,  DERE JEFF ,  STRAUB TED ,  FONSECA JORGE ,  BOO KUAN MOON ,  LAU SOO YONG

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/50855 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/143 ,  B01L2300/021 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1827 ,  B01L2300/1844

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens.

Abstract translation: 提供生物分析系统。 该系统包括被配置为容纳多个样本保持器中的任何一个的可互换组件，每个相应样本保持器被配置为接收多个样本。 该系统还包括配置成循环多个样品通过一系列温度的控制系统。 该系统还包括配置成检测从多个样品发射的荧光信号的光学系统。 该光学系统尤其可以包括单个场透镜，激励源，光学传感器和多个滤波器组件。 激发源可以是一个或多个发光二极管。 场镜可以是双凸透镜。

公开(公告)号：WO2013138724A3

公开(公告)日：2014-04-24

申请号：PCT/US2013032107

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PALLAS MICHAEL C ,  NURSE JAMES ,  LIM GARY ,  STRAUB THEODORE ,  FOSTER EVAN

IPC: B01L3/02

CPC classification number: G01N35/1002 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/0038 ,  B01J2219/00599 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  C40B60/14

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。

公开(公告)号：WO2013049702A2

公开(公告)日：2013-04-04

申请号：PCT/US2012058096

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： FREUDENTHAL JACOB K ,  DAHLHOFF GEOFFREY ,  MAHER KEVIN ,  CHEN MINGSONG ,  LIM GARY ,  DANEHY RONALD ,  FORTESCUE DAVID ,  SHEN MING ,  SOH WOON LIANG TERENCE ,  CHENG FRANCIS ,  WU RUOYUN ,  LEE THIAM CHYE ,  CHAPPELL GARY ,  LIM CHEE KIONG ,  DERE JEFF ,  STRAUB TED ,  FONSECA JORGE ,  BOO KUAN MOON ,  LAU SOO YONG

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/50855 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/143 ,  B01L2300/021 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1827 ,  B01L2300/1844

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens.

Abstract translation: 提供生物分析系统。 该系统包括被配置为容纳多个样品保持器中的任何一个的可互换组件，每个样品保持器被配置为接收多个样品。 该系统还包括被配置为使多个样品循环通过一系列温度的控制系统。 该系统还包括被配置为检测从多个样本发射的荧光信号的光学系统。 特别地，光学系统可以包括单个场透镜，激发源，光学传感器和多个滤光器组件。 激发源可以是一个或多个发光二极管。 场透镜可以是双凸透镜。

公开(公告)号：WO2013049659A3

公开(公告)日：2013-07-04

申请号：PCT/US2012058042

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： DANEHY RONALD ,  LIM GARY ,  FREUDENTHAL JACOB

IPC: B01L3/00 ,  C12M1/00

CPC classification number: C12Q1/6806 ,  B01L3/50851 ,  B01L3/50855 ,  B01L3/50857 ,  B01L2200/026 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0893 ,  B01L2300/161 ,  C12M23/12 ,  C12M23/38

Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.

Abstract translation: 包含多个生物样品的情况包含基底，基底和覆盖物。 基底附着于基底并且包括构造成接收一种或多种生物样品的多个反应区域。 盖子包括内表面并且构造成附接到基座，使得基座和盖子一起提供包含基板的空腔。 内表面包括中心区域和围绕中心区域设置的凹陷区域。 中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上从中心区域偏移。