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公开(公告)号：WO2016127124A3
公开(公告)日：2016-09-29
申请号：PCT/US2016016882
申请日：2016-02-05
发明人： CHU YONG , MARKS JEFFREY , FREUDENTHAL JACOB , WESSEL THOMAS , WOO DAVID
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method (100) for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration (102) to determine reaction site positions in an image. The method further includes performing a pure dye calibration (108) to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration (110) to determine a filter normalization factor. The method includes performing an RNase P validation (112) to validate the instrument is capable of distinguishing between two different quantities of sample.
摘要翻译： 在一个示例性实施例中，提供了一种用于校准仪器的方法（100）。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准（102）以确定图像中的反应位置位置。 该方法还包括通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来执行纯染料校准（108）以确定在每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准（110）以确定滤波器归一化因子。 该方法包括执行RNase P验证（112）以验证仪器能够区分两种不同数量的样品。
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公开(公告)号：WO2016127107A3
公开(公告)日：2016-11-03
申请号：PCT/US2016016856
申请日：2016-02-05
发明人： CHU YONG , MARKS JEFFREY , FREUDENTHAL JACOB , CHEN MINGSONG , TOH TIONG HAN , AGUANNO MAURO , LAU LIK SENG , LOH LIAN SENG , TEO KOK SIONG , CHU ZENG WEI , MATHERS XIN , UY MICHAEL , YEO HUEI STEVEN , BOO KUAN MOON , LEE WAY XUANG , KOO CHIN YONG , TEO WEI FUH , LAU SOO YONG , SHIN HON SIU , TAN ZEQI , WESSEL THOMAS , WOO DAVID
CPC分类号： B01L7/52 , B01L2200/145 , B01L2300/18 , B01L2300/1822 , C12Q1/6806 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/026 , G01N2021/6439 , G01N2035/00316 , G01N2035/00366 , G01N2201/061 , G01N2201/12
摘要： A biological analysis system (100) is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block (114) configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray (160) comprising a slide assembly, the tray (160) configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.
摘要翻译： 提供生物分析系统（100）。 该系统包括样品块组件。 样品块组件包括被配置为容纳样品架的样品块（114），样品保持器被配置为接收多个样品。 该系统还包括配置成使多个样品循环通过一系列温度的控制系统。 所述系统还包括自动托盘（160），其包括滑动组件，所述托盘（160）构造成可将样品块组件从关闭位置可逆地滑动到打开位置，以允许用户接近所述多个样品保持器。
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公开(公告)号：WO2014130970A3
公开(公告)日：2015-12-03
申请号：PCT/US2014018110
申请日：2014-02-24
发明人： CHEN MINGSONG , AGUANNO MAURO , BOO KUAN MOON , SOH WOON LIANG TERENCE , FREUDENTHAL JACOB , MARKS JEFFREY
CPC分类号： G01N21/6486 , G01N21/0332 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2201/061 , G01N2201/0624 , G01N2201/0627 , G01N2201/121
摘要： An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.
摘要翻译： 用于处理和/或测量生物过程的仪器包括样品处理系统，激发源，激发光学系统，光学传感器和发射光学系统。 样品处理系统被配置为保持第一样品保持器和第二样品保持器，其中样品池的数量对于每个样品保持器是不同的，或者第一样品池的特征尺寸与第二样品保持器的特征尺寸不同。 仪器还包括激发源温度控制器，其包括耦合到激发源的温度传感器。 温度控制器被配置为当第一样品保持器被仪器保持时产生第一目标温度，并且当第二样品保持器被仪器保持时产生第二目标温度。
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公开(公告)号：WO2013049659A3
公开(公告)日：2013-07-04
申请号：PCT/US2012058042
申请日：2012-09-28
发明人： DANEHY RONALD , LIM GARY , FREUDENTHAL JACOB
CPC分类号： C12Q1/6806 , B01L3/50851 , B01L3/50855 , B01L3/50857 , B01L2200/026 , B01L2200/0642 , B01L2200/0684 , B01L2200/142 , B01L2300/0819 , B01L2300/0829 , B01L2300/0858 , B01L2300/0893 , B01L2300/161 , C12M23/12 , C12M23/38
摘要： A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
摘要翻译： 包含多个生物样品的情况包含基底，基底和覆盖物。 基底附着于基底并且包括构造成接收一种或多种生物样品的多个反应区域。 盖子包括内表面并且构造成附接到基座，使得基座和盖子一起提供包含基板的空腔。 内表面包括中心区域和围绕中心区域设置的凹陷区域。 中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上从中心区域偏移。
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