公开(公告)号：WO2010005551A2

公开(公告)日：2010-01-14

申请号：PCT/US2009/003970

申请日：2009-07-07

Applicant: MASCOMA CORPORATION ,  BREVNOVA, Elena, E. ,  RAJGARHIA, Vineet ,  MELLON, Mark ,  WARNER, Anne ,  MCBRIDE, John ,  GANDHI, Chhayal ,  WISWALL, Erin

Inventor： BREVNOVA, Elena, E. ,  RAJGARHIA, Vineet ,  MELLON, Mark ,  WARNER, Anne ,  MCBRIDE, John ,  GANDHI, Chhayal ,  WISWALL, Erin

IPC: C07K14/435 ,  C12N1/16 ,  C12N9/42 ,  C12N15/81

CPC classification number: C12N9/2437 ,  C12N15/81 ,  C12N15/815 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16

Abstract: The present invention provides for heterologous expression of termite and termite- associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Abstract translation: 本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。 纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。 纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。 在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。 因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

公开(公告)号：WO2014035458A1

公开(公告)日：2014-03-06

申请号：PCT/US2013/000090

申请日：2013-03-15

Applicant: MASCOMA CORPORATION

Inventor： MCBRIDE, John, E. ,  WISWALL, Erin ,  SHIKHARE, Indraneel ,  XU, Haowen ,  THORNGREN, Naomi ,  HAU, Heidi, H. ,  STONEHOUSE, Emily

IPC: C12N9/24 ,  C12N15/81

CPC classification number: C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2491 ,  C12N9/2494 ,  C12P3/00 ,  C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12Y301/01006 ,  C12Y301/01072 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01072 ,  C12Y302/01131 ,  C12Y302/01139 ,  Y02E50/16

Abstract: The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae . In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Abstract translation: 本发明提供了一种多组分酶系统，其从硬木中水解半纤维素寡聚体，其可以在例如酿酒酵母（Saccharomyces cerevisiae）的酵母中表达。 在一些实施方案中，本发明提供一系列生物催化剂的工程化，其将该酶系统的组分的表达和分泌与强大的，快速的木糖利用和在工业上相关的工艺条件下的乙醇发酵相结合，用于固结的生物处理。 在一些实施方案中，本发明利用菌株的共培养物，其可以通过在发酵系统中引入另外的酶而实现显着改善的性能。

公开(公告)号：WO2010075529A2

公开(公告)日：2010-07-01

申请号：PCT/US2009/069443

申请日：2009-12-23

Applicant: MASCOMA CORPORATION ,  HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

Inventor： HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

IPC: C12P7/06

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention is directed to thermophilic gram-positive anaerobic host cells. The thermophilic gram-positive anaerobic host cells of the invention, for example Themoanaerobacterium sachharolyticum (" T. sacch "), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO 2 . In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T. sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Abstract translation: 本发明涉及嗜热革兰氏阳性厌氧宿主细胞。 本发明的嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“茶糖酵母”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如，可以遗传改变宿主细胞，以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产。

公开(公告)号：WO2011153516A2

公开(公告)日：2011-12-08

申请号：PCT/US2011/039192

申请日：2011-06-03

Applicant: MASCOMA CORPORATION ,  BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen ,  STELLENBOSCH UNIVERSITY

Inventor： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

CPC classification number: C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

Abstract: The present invention is directed to a. yeast strain, or strains, secreting a full suite, or any subset of that, full suite, of enzymes to hydrolyze com starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicelhiiose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

Abstract translation: 本发明涉及a。 酵母菌株或菌株，分泌全套或全套酶的任何子集，以水解com淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和木质素和碳水化合物之间的连接的酶）和 利用戊糖（木糖和阿拉伯糖）。 本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。 所得菌株或菌株可以同时水解淀粉和纤维素。 所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。 所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。 所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。 此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共同培养并用于从生物质原料生产乙醇。

公开(公告)号：WO2010096562A2

公开(公告)日：2010-08-26

申请号：PCT/US2010/024592

申请日：2010-02-18

Applicant: MASCOMA CORPORATION ,  MCBRIDE, John ,  MELLON, Mark ,  RAJGHARIA, Vineet ,  BREVNOVA, Elena, E. ,  WISWALL, Erin ,  HOGSETT, David, A. ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  VAN ZYL, Emile

Inventor： MCBRIDE, John ,  MELLON, Mark ,  RAJGHARIA, Vineet ,  BREVNOVA, Elena, E. ,  WISWALL, Erin ,  HOGSETT, David, A. ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  VAN ZYL, Emile

IPC: C12P7/10

CPC classification number: C12P7/10 ,  C07K14/33 ,  C07K2319/035 ,  C07K2319/70 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N15/81 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02P20/52

Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Abstract translation: 本发明涉及在微生物中异源纤维素酶体的工程和表达，以促进生物质转化为有用的产物。 在一些实施方案中，本发明涉及形成纤维素酶的核的骨架蛋白的表达。 纤维素酶或其他生物降解酶可以通过泊松特蛋白结构域 - 凝聚素结构域相互作用而非共价连接至支架蛋白。