公开(公告)号：WO2015023982A1

公开(公告)日：2015-02-19

申请号：PCT/US2014/051343

申请日：2014-08-15

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： SHAREI, Armon, R. ,  ADALSTEINSSON, Viktor, A. ,  CHO, Nahyun ,  LANGER, Robert, S. ,  LOVE, J., Christopher ,  JENSEN, Klavs, F.

IPC: B07B1/00

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2300/08 ,  B01L2400/0487 ,  B82Y30/00 ,  C12N5/0634 ,  C12N5/0693 ,  C12Q1/04 ,  G01N1/30 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

Abstract translation: 基于所述细胞的物理性质分离或鉴定细胞可以包括提供细胞悬浮液; 将所述悬浮液通过包括收缩的微流体通道; 使细胞悬液通过狭窄; 并使所述细胞悬浮液与化合物接触。 与相对较小的细胞相比，收缩部的尺寸可以优先变形相对较大的细胞。

公开(公告)号：WO2022159910A1

公开(公告)日：2022-07-28

申请号：PCT/US2022/013769

申请日：2022-01-25

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE GENERAL HOSPITAL CORPORATION

Inventor： LOVE, J., Christopher ,  MARTIN, Alonso, Maria Carmen ,  BHATIA, Sangeeta, N. ,  TABRIZI, Shervin ,  ADALSTEINSSON, Viktor, A. ,  XIONG, Kan

IPC: C12N15/10 ,  C08L101/12 ,  C12Q1/6825 ,  G01N33/551

Abstract: This disclosure provides a method for substantially increasing the concentration of cfDNA in a patient. By injecting a patient with lipid and/or polymer nanoparticles, agents that bind cfDNA, or inhibit deoxyribonucleases prior to collection of a sample of cfDNA, e.g., by way of a liquid biopsy, major pathways for the degradation of cfDNA are temporarily blocked, permitting transient accumulation of cfDNA. This strategy has the potential to dramatically enhance the quality of detection achieved by downstream cfDNA analytical applications, such as sequencing applications.