公开(公告)号：WO2018169901A1

公开(公告)日：2018-09-20

申请号：PCT/US2018/022093

申请日：2018-03-13

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LU, Timothy, Kuan-ta ,  NISSIM, Lior ,  WU, Ming-ru

IPC: C12N15/85 ,  C12N15/869 ,  A61K48/00

Abstract: Aspects of the present disclosure provide synthetic promoters that are differentially modulated between certain diseased cells (e.g., cancer cells) and normal cells (e.g., non-cancer cells). These synthetic promoters are useful, for example, for targeted expression of therapeutic molecules in diseased cells.

公开(公告)号：WO2019241684A1

公开(公告)日：2019-12-19

申请号：PCT/US2019/037269

申请日：2019-06-14

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  KELLIS, Manolis ,  NOVOA PARDO, Eva, Maria

Inventor： KELLIS, Manolis ,  NOVOA PARDO, Eva, Maria ,  LU, Timothy, Kuan-ta ,  CAO, Jicong ,  ZHANG, Zhizhuo

IPC: C12N15/10 ,  C12N15/67 ,  G16B15/00 ,  G16B30/00 ,  G16B40/00

Abstract: Disclosed herein are novel 5' UTR sequences that can be used to control gene expression in various contexts. Also disclosed herein are methods of engineering 5' UTR sequences and methods and kits for screening 5' UTR sequences for a property of interest.

公开(公告)号：WO2015156870A2

公开(公告)日：2015-10-15

申请号：PCT/US2015/011534

申请日：2015-01-15

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： DENG, Zhengtao ,  LU, Timothy, Kuan-ta

IPC: A61K49/00

CPC classification number: G01N33/521 ,  A61K49/0093

Abstract: Various aspects and embodiments provided herein are directed to compositions that include at least one nanoparticle linked to a first polypeptide, and a biologically synthesizable polymer linked to at least one second polypeptide that binds covalently to the first polypeptide. Other aspects and embodiments provided herein are directed to methods of producing the foregoing compositions and components therein.

Abstract translation: 本文提供的各个方面和实施方案涉及包含连接至第一多肽的至少一个纳米颗粒的组合物，以及与至少一种与第一多肽共价结合的第二多肽连接的生物可合成聚合物。 本文提供的其它方面和实施例涉及在其中生产前述组合物和组分的方法。

公开(公告)号：WO2016183438A1

公开(公告)日：2016-11-17

申请号：PCT/US2016/032348

申请日：2016-05-13

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LU, Timothy, Kuan-ta ,  PERLI, Samuel, David ,  CUI, Hao

IPC: C12N15/00 ,  C12N15/63 ,  G06N3/12

CPC classification number: C12N15/63 ,  C12N15/102 ,  C12N15/111 ,  C12N2310/20 ,  G06N3/12

Abstract: The present disclosure is directed, in some embodiments, to engineered nucleic acids comprising a promoter operably linked to a nucleotide sequence encoding a guide ribonucleic acid (gRNA) that comprises a specificity determining sequence (SDS) and a protospacer adjacent motif (PAM). The present disclosure is directed, in some embodiments, to cells comprising, vectors comprising, and methods of producing the engineered nucleic acids.

Abstract translation: 在一些实施方案中，本公开涉及包含与编码引导核糖核酸（gRNA）的核苷酸序列可操作地连接的启动子的工程化核酸，其包含特异性测定序列（SDS）和原原理邻近基序（PAM）。 在一些实施方案中，本公开涉及包含载体的细胞，以及生产工程化核酸的方法。

公开(公告)号：WO2014093852A1

公开(公告)日：2014-06-19

申请号：PCT/US2013/075068

申请日：2013-12-13

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LU, Timothy, Kuan-ta ,  SIUTI, Piro

IPC: G06N3/12 ,  C12N15/09 ,  C12N15/63

CPC classification number: G06N3/002

Abstract: The invention provides, inter alia, recombinase-based systems that provide for integrated logic and memory in living cells.

Abstract translation: 本发明尤其提供了提供活细胞中的集成逻辑和记忆的基于重组酶的系统。

公开(公告)号：WO2018013551A1

公开(公告)日：2018-01-18

申请号：PCT/US2017/041509

申请日：2017-07-11

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LU, Timothy, Kuan-ta ,  PEREZ-PINERA, Pablo

IPC: C07K14/39 ,  C12N15/10 ,  C12N15/81 ,  C12N15/90 ,  C12R1/84

CPC classification number: C07K16/10 ,  A61K39/00 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/41 ,  C12N15/10 ,  C12N15/63 ,  C12N15/81 ,  C12N15/815 ,  C12N15/90 ,  C12N15/905 ,  C12P21/02

Abstract: Described herein are methods and compositions for the rapid production of therapeutic molecules using an inducible cell culture system.

公开(公告)号：WO2016069913A1

公开(公告)日：2016-05-06

申请号：PCT/US2015/058099

申请日：2015-10-29

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： LU, Timothy, Kuan-ta ,  CARR, Peter, A. ,  ZAKERI, Bijan

IPC: C12N15/87 ,  C12N15/74 ,  C12N15/00 ,  C12Q1/68

CPC classification number: C12N15/74 ,  C12N15/87 ,  C12Q1/68 ,  G06F19/24 ,  C12Q2521/507 ,  C12Q2563/185

Abstract: The disclosure relates to the use of steganographic methods to camouflage information encoded on nucleic acids. Specifically, the method comprising preparing a pool of recombinant nucleic acid constructs, wherein at least one of the constnjcts comprises the nucleic acid sequence to be camouflaged and wherein the pool is heterogeneous with respect to the orientation of the nucleic acid sequence to be camouflaged, and the information is camouflaged using genetic recombination.

Abstract translation: 本公开涉及使用隐写术方法伪装编码在核酸上的信息。 具体而言，所述方法包括制备重组核酸构建体的一个池，其中，所述constnjcts中的至少一个包括所述核酸序列被伪装，并且其中所述池是异构的相对于所述核酸序列的取向被伪装，和 这些信息是通过基因重组伪装的。