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公开(公告)号：WO0075657A3
公开(公告)日：2001-09-27
申请号：PCT/EP0004948
申请日：2000-05-30
Applicant: MOLECULAR MACHINES & IND GMBH , ANSELL RICHARD , SEEGER STEFAN
Inventor： ANSELL RICHARD , SEEGER STEFAN
IPC: G01N21/64 , C40B30/04 , G01N33/52 , G01N33/566 , G01N33/68
CPC classification number: C40B30/04 , G01N33/52 , G01N33/6845 , G01N2500/20
Abstract: The invention relates to a method for screening target-ligand interactions while using a chemical library of ligands, to the chemical library of ligands as such, to a method for producing the chemical library, as well as to the use of the chemical library for developing active agents and for developing molecular sensors. The screening method comprises the following steps: (a) measuring at least one fluorescence property of a locally addressable chemical library of ligands that is immobilized on a solid phase, whereby a molecular fluorescence sensor is bound to each ligand; (b) adding the target, and; (c) measuring the same fluorescence property/properties of the chemical library as described in step (a). The locally addressable chemical library of ligands that is immobilized on a solid phase is characterized in that each ligand is bound to a molecular fluorescence sensor in a preferable manner in which the molecular fluorescence sensor is bound between the ligand and the solid phase and/or to the end of the ligand situated opposite the solid phase.
Abstract translation: 本发明涉及一种方法，用于筛选使用的配体的化学文库的配体作为这样的化学文库靶 - 配体相互作用，用于制备化学文库和用于药物发现中的用途的化学库的和分子的传感器的发展过程 ， 该筛选方法包括以下步骤：（a）测量的固定化在固相上的至少一个荧光特性，ortsadressierbaren配体其中，在每个配位体的分子荧光传感器附着的化学文库，（b）加入的目标，以及（c）测量所述 如在步骤中描述的化学文库的相同的荧光特性（一个或多个）（a）中。 固定化在固相上，ortsadressierbare配体的化学文库，其特征在于每个配体结合到一个分子荧光传感器，优选地使得所述配体和固相和/或分子之间的荧光传感器结合到所述固相上的相对的配体的端 是。
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公开(公告)号：WO2008132247A3
公开(公告)日：2010-03-11
申请号：PCT/EP2008058302
申请日：2008-06-27
Applicant: RUCKSTUHL THOMAS , SEEGER STEFAN
Inventor： RUCKSTUHL THOMAS , SEEGER STEFAN
CPC classification number: G01N21/0303 , B01L3/5027 , B01L3/5082 , B01L3/5085 , B01L2300/0654 , G01N21/645 , G01N21/6452 , G01N21/6458 , G01N2201/0642 , G02B2207/113
Abstract: The invention relates to a liquid receptacle (22) comprising a bottom (1) and sidewalls for holding a liquid. The bottom (1) encompasses a flat sensor surface (3) that is in contact with the liquid when the receptacle is filled, a light-incident area (2) that is located below the sensor surface (3) and is suitable for focusing light (13) onto the sensor surface (3), a light emergence area (5), and a cover area (4) that is suitable for reflecting light from the sensor surface (3) such that the light can emerge through the light emergence area (5). The invention further relates to a method for qualitatively or quantitatively determining an analyte in such a liquid receptacle (22). In said method, excitation light (13) is focused onto the sensor surface (3) via the light-incident area (2) such that a luminescent marker which characterizes the analyte is excited, and the generated luminescence is then reflected onto the cover surface (4) and is detected after emerging through the light emergence area (5). The invention also relates to an analysis device comprising a holder for a liquid receptacle, a light source (10) that is disposed such that the light (13) thereof can be focused onto the sensor surface (3) of the liquid receptacle (22) via the light-incident area (2), and a detector (17) which is arranged in such a way as to be able to detect the light (9) emerging from the light emergence area (5) of the liquid receptacle (22).
Abstract translation: 本发明涉及一种液体容器（22）包括一个底部（1）和侧壁用于容纳液体，所述基座（1），包括：平坦的传感器表面（3），其与接触所述液体填充; 光入射面（2）的传感器表面（3）所示的适合于将光聚焦（13）的传感器表面（3）上; 光出射表面（5）; 因此，它可以退出和外侧表面（4），其适于通过所述光出射面以反射来自所述传感器表面（3）的光（5）。 此外，本发明涉及用于定性或定量测定分析物的方法，在这样一个液体容器（22）的激发光（13）聚焦在传感器表面（3）上的光入射面（2）上，从而特性的分析物发光标记物被激发，然后将 被反射地幔表面（4），从而产生的发光和新兴通过光出射面（5）之后检测到。 本发明还涉及一种包括用于液体容器的保持器的分析装置; 一个光源（10），其被布置成使得它的光（13）在所述光入射面（2）的传感器表面（3）上的液体容器（22）的可聚焦， - 和一个检测器（17），它是设置 它可以从液体容器的出射表面（5）检测所述光（22）射出的光（9）。
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公开(公告)号：WO2008132247A8
公开(公告)日：2010-01-07
申请号：PCT/EP2008058302
申请日：2008-06-27
Applicant: RUCKSTUHL THOMAS , SEEGER STEFAN
Inventor： RUCKSTUHL THOMAS , SEEGER STEFAN
CPC classification number: G01N21/0303 , B01L3/5027 , B01L3/5082 , B01L3/5085 , B01L2300/0654 , G01N21/645 , G01N21/6452 , G01N21/6458 , G01N2201/0642 , G02B2207/113
Abstract: The invention relates to a liquid receptacle comprising a bottom and sidewalls for holding a liquid. The bottom encompasses a flat sensor surface that is in contact with the liquid when the receptacle is filled, a light-incident area that is located below the sensor surface and is suitable for focusing light onto the sensor surface, a light emergence area, and a cover area that is suitable for reflecting light from the sensor surface such that the light can emerge through the light emergence area. The invention further relates to a method for qualitatively or quantitatively determining an analyte in such a liquid receptacle. In said method, excitation light is focused onto the sensor surface via the light-incident area such that a luminescent marker which characterizes the analyte is excited, and the generated luminescence is then reflected onto the cover surface and is detected after emerging through the light emergence area. The invention also relates to an analysis device comprising a holder for a liquid receptacle, a light source that is disposed such that the light thereof can be focused onto the sensor surface of the liquid receptacle via the light-incident area, and a detector which is arranged in such a way as to be able to detect the light emerging from the light emergence area of the liquid receptacle.
Abstract translation: 本发明涉及一种包括用于容纳液体，所述基部包括底部和侧壁的液体容器：平坦的传感器表面，其与接触所述液体填充; 传感器表面，其适于将光聚焦到所述传感器表面下方的光入射表面上; 光出射表面; 并且其适于横向表面，以反射从所述传感器表面的光，以便它可以通过光出射面出射。 此外，本发明涉及用于定性或定量测定在这样的液体容器，其中，所述激发光经由光输入表面聚焦在传感器表面上的分析物的方法，由此特性，分析物发光标记物被激发，然后反映了如此得到的覆盖表面，并通过退出后的发光 被检测光发射表面。 本发明还涉及一种包括用于液体容器的保持器的分析装置; 其被布置为使得它们的光可以聚焦通过所述光入射面的液体容器的传感器表面上的光源; 并且被布置为使得它可以检测排出的液体容器的光的光出射面的光的检测器。
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