公开(公告)号：WO2019161144A1

公开(公告)日：2019-08-22

申请号：PCT/US2019/018128

申请日：2019-02-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: A01G7/06 ,  A01H1/00 ,  A01H5/00 ,  C12N15/11

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：WO2020243363A1

公开(公告)日：2020-12-03

申请号：PCT/US2020/034996

申请日：2020-05-28

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: A01H5/10 ,  C12N15/82 ,  A01H1/00 ,  C12N15/00

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of a specific GA20 oxidase gene or locus to produce a genomic deletion or disruption that brings an antisense sequence of the GA20 oxidase gene under the control of a neighboring SAMT gene promoter. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：WO2019161147A1

公开(公告)日：2019-08-22

申请号：PCT/US2019/018131

申请日：2019-02-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: C12N15/82 ,  C12N15/00 ,  C12N15/63

Abstract: The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.

公开(公告)号：WO2020117837A1

公开(公告)日：2020-06-11

申请号：PCT/US2019/064282

申请日：2019-12-03

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： CARGILL, Edward ,  MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda ,  VALENTINE, Michelle

IPC: A01H1/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/79 ,  C12N9/22

Abstract: Provided herein are novel methods and compositions for producing corn plants that have improved digestibility. Also provided are corn plants, corn plant tissues and corn plant seeds that contain novel edited Brown midrib 3 (Bmr3) genes and proteins, as well as methods of producing the same.

公开(公告)号：WO2016172051A3

公开(公告)日：2016-10-27

申请号：PCT/US2016/028130

申请日：2016-04-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BROWER-TOLAND, Brent ,  CALDO, Rico A. ,  DAI, Shunhong ,  GABBERT, Karen ,  GOLDSHMIDT, Alexander ,  HOWELL, Miya D. ,  KARUNANANDAA, Balasulojini ,  MANJUNATH, Sivalinganna ,  MCDILL, Bradley W. ,  OVADYA, Daniel J. ,  PREUSS, Sasha ,  RICE, Elena A. ,  SAVIDGE, Beth ,  SHARMA, Vijay K.

IPC: A01H1/00 ,  A01H1/02 ,  A01H5/00

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

Abstract translation: 本发明提供了重组DNA构建体，载体和分子，所述重组DNA构建体，载体和分子包含与营养阶段启动子可操作连接的编码成花FT蛋白的多核苷酸序列，所述营养阶段启动子也可以是分生组织优选的或分生组织特异性启动子。 还提供了包含编码成花FT蛋白的多核苷酸序列的转基因植物，植物细胞和组织以及植物部分。 相对于对照或野生型植物，包含成花FT转基因的转基因植物可在转基因植物上，特别是在植物的主茎上产生更多的铃铛，角果，果实，坚果或荚果。 进一步提供了用于将成花FT转基因引入植物中的方法，以及在包括更高密度的田间种植转基因FT植物。 因此，本发明的转基因植物可提供比野生型植物更高的产量潜力，并且由于其改变的植物结构可以以更高的密度种植。

公开(公告)号：WO2019161149A1

公开(公告)日：2019-08-22

申请号：PCT/US2019/018133

申请日：2019-02-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: C12N15/82 ,  A01G7/06

Abstract: The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.

公开(公告)号：WO2019161147A9

公开(公告)日：2019-08-22

申请号：PCT/US2019/018131

申请日：2019-02-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: C12N15/82 ,  C12N15/00 ,  C12N15/63

Abstract: The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.

公开(公告)号：WO2010039750A3

公开(公告)日：2010-04-08

申请号：PCT/US2009/058908

申请日：2009-09-30

Applicant: MONSANTO TECHNOLOGY LLC ,  AASEN, Eric ,  ABAD, Mark, S. ,  ALLEN, Edwards ,  AMBIKA, Thandoni, Rao ,  ANIL, Veena, S. ,  AUGUSTINE, Alice ,  BACK, Stephanie ,  BADAMI, Pranesh ,  BASRA, Amarjit ,  BRUSTAD, Kraig ,  DENG, Molian ,  DEZWAAN, Todd ,  DIETRICH, Charles, R. ,  DUFF, Stephen, M. ,  GABBERT, Karen, K ,  HE, Steve ,  HENDRIX, Bill ,  JIA, Hongwu ,  KAMMARADI RAMANATH BHAT, Vidya ,  KARUNANANDAA, Balasulojini ,  KOSOLA, Kevin, R. ,  KRISHNAMURTHY, K. ,  KUMAR, Ganesh ,  KURIAKOSE, Saritha ,  LAKKANNA, Shantala ,  LEIBMAN, Mark ,  LUTFIYYA, Linda, L. ,  MADAPPA, Savitha ,  MANJUNATH, Sivalinganna ,  MEISTER, Robert, Joseph ,  PRASAD, Bellur, Narasimha ,  PREUSS, Sasha ,  QI, Qunqang ,  RAMACHANDRA, Dhanalakshmi ,  RAYCHAUDHURI, Aniruddha ,  SANGEETHA, S. ,  SARACCO, Scott ,  SHARMA, Chitresh ,  SHARMA, Vijay ,  SOMAIAH, Rajani, Monnanda ,  SUDARSHANA, Padmini ,  THOMPSON-MIZE, Rebecca ,  VENKATACHALAYYA, Srikanth Babu ,  VENKATESH, Tyamagondlu V. ,  WANG, Huai ,  WU, Jingrui ,  WU, Xiaoyun ,  YANG, Sean ,  ZENG, Qin ,  ZHAO, Jianmin

Inventor： AASEN, Eric ,  ABAD, Mark, S. ,  ALLEN, Edwards ,  AMBIKA, Thandoni, Rao ,  ANIL, Veena, S. ,  AUGUSTINE, Alice ,  BACK, Stephanie ,  BADAMI, Pranesh ,  BASRA, Amarjit ,  BRUSTAD, Kraig ,  DENG, Molian ,  DEZWAAN, Todd ,  DIETRICH, Charles, R. ,  DUFF, Stephen, M. ,  GABBERT, Karen, K ,  HE, Steve ,  HENDRIX, Bill ,  JIA, Hongwu ,  KAMMARADI RAMANATH BHAT, Vidya ,  KARUNANANDAA, Balasulojini ,  KOSOLA, Kevin, R. ,  KRISHNAMURTHY, K. ,  KUMAR, Ganesh ,  KURIAKOSE, Saritha ,  LAKKANNA, Shantala ,  LEIBMAN, Mark ,  LUTFIYYA, Linda, L. ,  MADAPPA, Savitha ,  MANJUNATH, Sivalinganna ,  MEISTER, Robert, Joseph ,  PRASAD, Bellur, Narasimha ,  PREUSS, Sasha ,  QI, Qunqang ,  RAMACHANDRA, Dhanalakshmi ,  RAYCHAUDHURI, Aniruddha ,  SANGEETHA, S. ,  SARACCO, Scott ,  SHARMA, Chitresh ,  SHARMA, Vijay ,  SOMAIAH, Rajani, Monnanda ,  SUDARSHANA, Padmini ,  THOMPSON-MIZE, Rebecca ,  VENKATACHALAYYA, Srikanth Babu ,  VENKATESH, Tyamagondlu V. ,  WANG, Huai ,  WU, Jingrui ,  WU, Xiaoyun ,  YANG, Sean ,  ZENG, Qin ,  ZHAO, Jianmin

IPC: C12N15/82

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

公开(公告)号：WO2020243361A1

公开(公告)日：2020-12-03

申请号：PCT/US2020/034993

申请日：2020-05-28

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： MANJUNATH, Sivalinganna ,  RYMARQUIS, Linda A. ,  SLEWINSKI, Thomas L.

IPC: G01F1/56 ,  C12N15/8213 ,  C12N15/8218 ,  C12N15/8262 ,  C12N15/8297 ,  C12N9/0071

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of specific subtypes of GA20 oxidase genes to introduce an antisense sequence or segment into the gene. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：WO2015054106A1

公开(公告)日：2015-04-16

申请号：PCT/US2014/059253

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： ABAD, Mark, Scott ,  ALLEN, Edwards, M. ,  ANIL, Veena, S. ,  AUGUSTINE, Alice, Clara ,  BANKS, Isaac, R. ,  BHAT, Prasanna, R. ,  CHITTOOR-VIJAYANATH, Jaishree, M. ,  CHOMET, Paul, S. ,  DENG, Molian ,  DUFF, Stephen ,  GOLDMAN, Barry, S. ,  IVASHUTA, Sergey ,  KARUNANANDAA, Balasulojini ,  LUTFIYYA, Linda ,  MANJUNATH, Sivalinganna ,  NEELAM, Anil ,  RAJANI, Monnanda, S. ,  RAMAMOHAN, G. ,  SHARMA, Chitresh ,  SHOBHA, Char ,  VENKATESH, Tyamagondlu V. ,  VIDYA, Kammaradi R. ,  ZHAO, Jianmin

IPC: A01H4/00

CPC classification number: C12N15/8241 ,  A01H1/02 ,  C12N15/113 ,  C12N15/8261 ,  C12N15/8271 ,  C12N2310/141

Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Abstract translation: 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这种转基因植物产生杂种种子的方法，种植这种种子并选择具有增强特征的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。