POLYPEPTIDES HAVING PHYTASE ACTIVTY AND POLYNUCLEOTIDES ENCODING SAME
    1.
    发明申请
    POLYPEPTIDES HAVING PHYTASE ACTIVTY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
    具有植酸酶活性的多肽和编码该多肽的多核苷酸

    公开(公告)号:WO2008092901A2

    公开(公告)日:2008-08-07

    申请号:PCT/EP2008/051137

    申请日:2008-01-30

    Applicant: NOVOZYMES A/S

    CPC classification number: C12N9/16 A23L33/18 C12N15/8243 C12P7/06 Y02E50/17

    Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo . The invention also relates to isolated polynucleotides encodingthe polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

    Abstract translation: 本发明涉及具有肌醇六磷酸酶活性的多肽。 这些多肽具有与源自细菌布丘氏菌的三种肌醇六磷酸酶中的任一种具有至少70%同一性的氨基酸序列,并且在所示位置包含至少一个以下氨基酸:119N,120L和/或121E。 这些植酸酶具有改善的比活性。 还公开了另外的特定氨基酸取代,其表征和区分具有改善的性质例如温度和/或pH稳定性,pH活性曲线,温度活性曲线,底物曲线,体外动物饲料中改善的性能的本发明的另外的肌醇六磷酸酶 in vivo 。 本发明还涉及编码所述多肽的分离的多核苷酸,包含所述多核苷酸的核酸构建体,载体和宿主细胞以及生产和使用所述多肽的方法。

    POLYPEPTIDES HAVING ISOAMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
    9.
    发明申请
    POLYPEPTIDES HAVING ISOAMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
    具有异氰酸酯酶活性的多肽和编码其的多核苷酸

    公开(公告)号:WO2011020910A1

    公开(公告)日:2011-02-24

    申请号:PCT/EP2010/062164

    申请日:2010-08-20

    Applicant: NOVOZYMES A/S

    Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.

    Abstract translation: 本发明涉及具有衍生自日本黛麻的异淀粉酶活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体,载体和宿主细胞以及产生和使用多肽的方法。 本发明还涉及具有异淀粉酶活性的所述多肽用于生产葡萄糖浆,果糖糖浆,麦芽糖糖浆或麦芽糖醇的用途。

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