公开(公告)号：WO2019032576A2

公开(公告)日：2019-02-14

申请号：PCT/US2018/045597

申请日：2018-08-07

Applicant: THE CHARLES STARK DRAPER LABORATORY INC.

Inventor： MCFARLAND, Kirsty A. ,  ROGERS, Miles T. ,  MCBRINE, Connor ,  HOLDER, Jason

IPC: C12N7/02 ,  C12N15/66

CPC classification number: C12N7/02 ,  C12N7/025 ,  C12N15/1031 ,  C12N15/63 ,  C12N15/66 ,  C12N2795/00041 ,  C12N2800/202 ,  C12Q2521/101 ,  C12Q2521/319 ,  C12Q2521/501

Abstract: The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

公开(公告)号：WO2019032576A3

公开(公告)日：2019-02-14

申请号：PCT/US2018/045597

申请日：2018-08-07

Applicant: THE CHARLES STARK DRAPER LABORATORY INC.

Inventor： MCFARLAND, Kirsty A. ,  ROGERS, Miles T. ,  MCBRINE, Connor ,  HOLDER, Jason

IPC: C12N7/02 ,  C12N15/66

Abstract: The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

公开(公告)号：WO2019068006A1

公开(公告)日：2019-04-04

申请号：PCT/US2018/053582

申请日：2018-09-28

Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.

Inventor： MCFARLAND, Kirsty A. ,  MAGYAR, Andrew P. ,  PANG, Ting

IPC: C07K14/435 ,  C12N15/09

CPC classification number: C12N15/09 ,  C07K14/435

Abstract: Stimulus-controlled cell lysis compositions and methods of their use are described.

公开(公告)号：WO2019075321A1

公开(公告)日：2019-04-18

申请号：PCT/US2018/055600

申请日：2018-10-12

Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.

Inventor： MCFARLAND, Kirsty A. ,  LISSANDRELLO, Charles A. ,  MAGYAR, Andrew P. ,  ROSENBERGER, Erin

IPC: B01L3/00

CPC classification number: B01L3/50853 ,  B01L2200/0689 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0896

Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.

公开(公告)号：WO2018217689A1

公开(公告)日：2018-11-29

申请号：PCT/US2018/033798

申请日：2018-05-22

Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.

Inventor： MAGYAR, Andrew P. ,  CAVANAGH, Peter ,  ROGERS, Miles ,  MCFARLAND, Kirsty A. ,  SPRACHMAN, Melissa ,  BILLINGS-SIUTI, Amanda Nicole ,  RAUSTAD, Nicole E.

IPC: C12P19/34 ,  C12N9/12 ,  C07C245/08

CPC classification number: C12P19/34 ,  C07C245/08 ,  C07C247/04 ,  C12N9/1264

Abstract: Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3' end of a growing single-stranded DNA polynucleotide.

公开(公告)号：WO2020028766A1

公开(公告)日：2020-02-06

申请号：PCT/US2019/044827

申请日：2019-08-02

Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.

Inventor： FRANK, Ian W. ,  MAGYAR, Andrew P. ,  LARSEN, Cory ,  UNG, Jonathan S. ,  RUSSELL, Kasey J. ,  MCFARLAND, Kirsty A. ,  KORN, Jeffrey A.

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686 ,  G01N21/64

Abstract: A well reader and actuator (100) for a well array (50) has an array of detectors, each detector for detecting a fluorescence signal from fluorophores in a respective well of the well array and an excitation subsystem for exciting the fluorophores in the wells of the well array. Embodiments of this invention can be used to carry out two important functions in a highly parallel manner: by addressing individual wells in a 32, 96, 384 etc. well array, where each well contains a potential chemical or biological reaction. The two functions are: 1) through thermal, optical or other means and combinations thereof the rate of a chemical or biological reaction is controlled or gated (e.g. colder wells inhibit a reaction, or an enzymatic reaction requires blue light to proceed); and 2) through use of fluorescent species that are sensitive to the target reaction - or reactions - an optical readout of fluorescent intensity and/or lifetime is tracked to monitor the evolution of the reaction.

公开(公告)号：WO2019139942A1

公开(公告)日：2019-07-18

申请号：PCT/US2019/012835

申请日：2019-01-09

Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.

Inventor： VEDULA, Else M. ,  MCFARLAND, Kirsty A. ,  BILLINGS-SIUTI, Amanda Nicole ,  MAGYAR, Andrew P.

IPC: G01N33/50 ,  C12Q1/18 ,  B01J19/00

CPC classification number: G01N33/5038 ,  C12M1/00 ,  C12Q1/18 ,  G01N33/5014 ,  G01N2500/10

Abstract: A method for identifying an interaction between a pathogen and a biological agent includes providing a platform for supporting cell growth, seeding different interaction sites on the platform with different biological agents, perfusing the platform with a fluid that carries substances for promoting growth and maintenance of the cells, exposing all of the interaction sites to a solution containing viruses, and detecting evidence indicative of the interaction, the evidence comprising evidence indicative of a change in structure or composition of a medium at the interaction site. The biological agent includes cells alone or cells with another substance.