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    ARRAY OF MICROMOLDED STRUCTURES FOR SORTING ADHERENT CELLS
    2.
    发明申请
    ARRAY OF MICROMOLDED STRUCTURES FOR SORTING ADHERENT CELLS 审中-公开
    用于分离附着细胞的微结构阵列

    公开(公告)号：WO2011103143A1

    公开(公告)日：2011-08-25

    申请号：PCT/US2011/025018

    申请日：2011-02-16

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  ALLBRITTON, Nancy ,  SIMS, Christopher ,  WANG, Yuli

    Inventor： ALLBRITTON, Nancy ,  SIMS, Christopher ,  WANG, Yuli

    IPC: C12M3/00

    CPC classification number: C12M23/12 ,  B01J19/0046 ,  B01J2219/00452 ,  B01J2219/00743 ,  C12M23/20 ,  C12M23/22 ,  C12M25/04 ,  C12M25/16 ,  C12M47/02 ,  C12M47/04

    Abstract: An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

    Abstract translation: 用于收集或培养细胞或细胞集落的装置包括：由柔性弹性聚合物材料形成且在其中形成有多个孔的共同基底; 以及可释放地连接到所述公共基板的多个刚性电池载体，所述载体以阵列的形式布置，并且每个载体弹性地容纳在其中一个孔中。 使用这种装置收集或培养细胞或细胞集落的方法是通过在装置上沉积携带细胞的液体培养基进行的，以使所述细胞沉降或粘附到所述载体上; 然后（c）通过从其被接收的空腔（例如通过用探针推动）逐渐地将释放能量释放到每个载体上来释放至少一个具有所述电池的所选载流子。

    METHODS TO GENERATE GASTROINTESTINAL EPITHELIAL TISSUE CONSTRUCTS
    3.
    发明申请
    METHODS TO GENERATE GASTROINTESTINAL EPITHELIAL TISSUE CONSTRUCTS 审中-公开
    方法生成胃肠上皮组织结构

    公开(公告)号：WO2016123474A1

    公开(公告)日：2016-08-04

    申请号：PCT/US2016/015631

    申请日：2016-01-29

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

    Inventor： ALLBRITTON, Nancy ,  WANG, Yuli ,  SIMS, Christopher ,  MAGNESS, Scott ,  BULTMAN, Scott

    IPC: C12N5/07 ,  C12N5/0789 ,  C12Q1/08

    CPC classification number: C12N5/0697 ,  C12N5/0679 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/00 ,  C12N2537/10 ,  C12Q1/08 ,  G01N2500/10

    Abstract: A method of making a live cell construct is carried out by: (a) providing a non-cellular support having a top surface and a bottom surface, (b) contacting live undifferentiated cells to the non-cellular support, and then (c) propagating a gastrointestinal epithelial cell monolayer on said top surface. In some embodiments, the live cells in the monolayer include: (i) undifferentiated cells (e.g., stem or progenitor cells); and (ii) optionally, but in some embodiments preferably, differentiated cells (e.g., enterocytes, Paneth cells, enteroendocrine cells, tuft cells, microcells, intra-epithelial lymphocytes, and/or goblet cells). Constructs formed by such methods and methods of using the same (e.g., in high through-put screening) are also described.

    Abstract translation: 通过以下步骤进行制备活细胞构建体的方法：（a）提供具有顶表面和底表面的非细胞支持体，（b）将活的未分化细胞与非细胞载体接触，然后（c） 在所述顶表面上传播胃肠上皮细胞单层。 在一些实施方案中，单层中的活细胞包括：（i）未分化细胞（例如干细胞或祖细胞）; 和（ii）任选地，但在一些实施方案中优选分化细胞（例如肠细胞，Paneth细胞，肠内分泌细胞，簇绒细胞，微细胞，上皮内淋巴细胞和/或杯状细胞）。 还描述了通过这样的方法和使用它们的方法形成的构造物（例如，在高通量筛选中）。

    SYSTEMS AND METHODS FOR ISOLATING CELLS IN CELL COLONIES IN CULTURE
    4.
    发明申请
    SYSTEMS AND METHODS FOR ISOLATING CELLS IN CELL COLONIES IN CULTURE 审中-公开
    用于在细胞培养中分离细胞的系统和方法

    公开(公告)号：WO2010068743A1

    公开(公告)日：2010-06-17

    申请号：PCT/US2009/067482

    申请日：2009-12-10

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei ,  SHADPOUR, Hamed

    Inventor： ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei ,  SHADPOUR, Hamed

    IPC: C12Q1/24

    CPC classification number: C12Q1/24 ,  B01L3/5025 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0609 ,  B01L2300/0819

    Abstract: Selecting and propagating a cell colony of interest from among a plurality of cell colonies carried on a common substrate in culture is carried out by: (a) selecting a cell colony of interest from among the plurality of cell colonies; (b) isolating a cell subset from the cell colony of interest; (c) analyzing (for example, by a destructive analysis) the cell subset isolated from the cell colony of interest to confirm the presence or absence of a desired feature therein; and then (d) propagating the cell colony of interest when the desired feature is present in the cell subset. A micropallet apparatus may include: (a) a substrate; (b) a plurality of discrete arrays formed on the substrate, each of the arrays comprising a plurality of releasable pallets, and (c) a plurality of gap forming regions, wherein the gap forming regions surround the pallets and separate the pallets from one another.

    Abstract translation: 通过以下步骤进行选择和繁殖培养中携带在共同底物上的多个细胞集落中的感兴趣的细胞集落：（a）从多个细胞集落中选择感兴趣的细胞集落; （b）从感兴趣的细胞集落分离细胞亚群; （c）分析（例如，通过破坏性分析）从感兴趣的细胞集落分离的细胞亚群以确认其中存在或不存在所需特征; 然后（d）当所需特征存在于细胞子集中时，传播感兴趣的细胞集落。 微孔装置可以包括：（a）基底; （b）形成在所述基板上的多个分立阵列，每个所述阵列包括多个可释放的托盘，以及（c）多个间隙形成区域，其中所述间隙形成区域围绕所述托盘并将所述托盘彼此分离 。

    METHOD OF CREATING REMOVABLE BARRIERS IN MICROFABRICATED FLUIDIC DEVICES
    5.
    发明申请
    METHOD OF CREATING REMOVABLE BARRIERS IN MICROFABRICATED FLUIDIC DEVICES 审中-公开

    公开(公告)号：WO2010016916A3

    公开(公告)日：2010-02-11

    申请号：PCT/US2009/004510

    申请日：2009-08-06

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei ,  BEAR, James ,  UETRECHT, Andrea

    Inventor： ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei ,  BEAR, James ,  UETRECHT, Andrea

    IPC: G01N35/00 ,  G01N37/00 ,  C12Q1/02

    Abstract: A method for creating a removable barrier in a microfluidic apparatus is carried out by: (a) providing a microfluidic apparatus having at least a first chamber and a second chamber, each including at least an inlet, wherein the first and second chamber are separated from one another by a plurality of barrier elements, the plurality of barrier elements defining a barrier zone, (b) introducing a liquid sample into the apparatus through the inlet of the first or second chamber to create a retained continuous liquid phase in one chamber, a continuous gas phase in the other chamber, and a gas/liquid interface between the first and second chamber at the barrier zone, (c) releasing the interface from the barrier elements, and (d) advancing the liquid phase past the barrier zone into the gas chamber displacing the gas phase. In some embodiments the barrier elements are hydrophobic (for example, the barrier elements comprise a hydrophobic polymer. Devices such as chemotaxis devices incorporating such methods are also described.

    METHODS TO GENERATE POLYMER SCAFFOLDS HAVING A GRADIENT OF CROSSLINKING DENSITY
    6.
    发明申请
    METHODS TO GENERATE POLYMER SCAFFOLDS HAVING A GRADIENT OF CROSSLINKING DENSITY 审中-公开
    产生梯度交联密度的聚合物支架的方法

    公开(公告)号：WO2018022548A1

    公开(公告)日：2018-02-01

    申请号：PCT/US2017/043601

    申请日：2017-07-25

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

    Inventor： ALLBRITTON, Nancy ,  WANG, Yuli ,  GUNASEKARA, Hennayaka Mudiyanselage Dulan ,  SIMS, Christopher

    IPC: C12N5/00

    Abstract: The present invention is directed to a method of making a live cell construct or a support, comprising: (a) providing a non-cellular organic polymer support having a top surface, a bottom surface, and an intermediate portion there between, and (b) contacting a cross-linking agent to one surface of said support for a time sufficient to generate a gradient of cross-linking of said polymer in said intermediate portion. Also provided are live cell constructs, supports, and methods of use of the supports and live cell constructs.

    Abstract translation: 本发明涉及制造活细胞构建体或载体的方法，其包括：（a）提供具有顶表面，底表面和底表面的非细胞有机聚合物载体 （b）使交联剂与所述载体的一个表面接触一段足以在所述中间部分产生所述聚合物交联梯度的时间。 还提供活细胞构建体，支持体以及使用支持体和活细胞构建体的方法。

    METHOD AND DEVICE FOR CELL SELECTION AND COLLECTION IN AN ISOLATED CULTURING ENVIRONMENT
    8.
    发明申请
    METHOD AND DEVICE FOR CELL SELECTION AND COLLECTION IN AN ISOLATED CULTURING ENVIRONMENT 审中-公开
    用于分离培养环境中细胞选择和收集的方法和装置

    公开(公告)号：WO2010093766A1

    公开(公告)日：2010-08-19

    申请号：PCT/US2010/023854

    申请日：2010-02-11

    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei

    Inventor： ALLBRITTON, Nancy ,  SIMS, Christopher ,  XU, Wei

    IPC: C12M1/22

    CPC classification number: C12M25/04 ,  C12M23/16

    Abstract: An apparatus for collecting or culturing cells or cell colonies has a common substrate and a plurality of cell carriers releasably connected to the common substrate. The carriers are arranged in the form of an array. The invention employs microcups as the cell carriers. The substrate is preferably free of barriers between the microcups, and in some embodiments the microcups have porous walls. Methods of using the apparatus are also described.

    Abstract translation: 用于收集或培养细胞或细胞集落的装置具有公共基底和可释放地连接到共同基底的多个细胞载体。 载体以阵列的形式排列。 本发明采用微型杯作为细胞载体。 基底优选在微杯之间不存在阻挡层，并且在一些实施例中，微型杯具有多孔壁。 还描述了使用该装置的方法。

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