公开(公告)号：WO2021254934A1

公开(公告)日：2021-12-23

申请号：PCT/EP2021/065904

申请日：2021-06-14

Applicant: DNA SCRIPT

Inventor： MARTIN, Carl ,  ARTIGUE, Marc ,  CRETON, Sandrine ,  DE CROZALS, Gabriel ,  GODRON, Xavier ,  HORGAN, Adrian ,  LACHAIZE, Henri ,  LUCKY, John ,  PEPONNET, Christine ,  YBERT, Thomas

IPC: B01J19/00 ,  C12P19/34 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00452 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00722

Abstract: The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

公开(公告)号：WO2021133768A1

公开(公告)日：2021-07-01

申请号：PCT/US2020/066547

申请日：2020-12-22

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： GEORGE, Wayne N. ,  BOUTELL, Jonathan Mark ,  LESSARD-VIGER, Mathieu ,  NIRANTAR, Saurabh ,  GATTI LAFRANCONI, Pietro ,  MCDONALD, Seth ,  TEO, Yin Nah ,  BACIGALUPO, Maria Rogert ,  PEISAJOVICH, Sergio ,  BRUSTAD, Eric ,  HOWARD, Michael ,  YANG, Xiangyuan ,  BOWEN, M. Shane ,  NEMIROSKI, Alex ,  BRODIN, Jeffrey ,  VINCENT, Ludovic ,  BERTI, Lorenzo ,  BROWN, Andrew A. ,  SHEN, Fei ,  STORMS, Lena ,  BENICE, Olivia

IPC: C12Q1/6869 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00608 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2565/513

Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle, in particular in sequencing by synthesis.

公开(公告)号：WO2022072274A1

公开(公告)日：2022-04-07

申请号：PCT/US2021/052178

申请日：2021-09-27

Applicant: ILLUMINA, INC.

Inventor： KODIRA CARIAPPA, Brinda ,  FISHER, Jeffrey S. ,  HONG, Sahngki ,  KRAFT, Lewis J. ,  MATHER, Brian D. ,  MONTANO-MACHADO, Vanessa

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  G01N21/05 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00608 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01L2300/0887 ,  B01L2300/165 ,  B01L3/502707 ,  G01N2021/7763 ,  G03F7/0037 ,  G03F7/0382 ,  G03F7/162 ,  G03F7/168 ,  G03F7/2004 ,  G03F7/2016 ,  G03F7/322

Abstract: In one example, a flow cell includes a base support and a protrusion over the base support, where the protrusion is a different material than the base support. The flow cell further includes a first functionalized layer over a first portion of the protrusion, a second functionalized layer over a second portion of the protrusion, and first and second primer sets respectively attached to the first and second functionalized layers.

公开(公告)号：WO2022010934A2

公开(公告)日：2022-01-13

申请号：PCT/US2021/040567

申请日：2021-07-06

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： LACKEY, Jeremy ,  INDERMUEHLE, Scott ,  DODD, David ,  PITSCH, Stefan

IPC: C08G61/02 ,  C08G61/12 ,  C09K11/06 ,  B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00711 ,  B01J2219/00722

Abstract: Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable LED arrays to control polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.

公开(公告)号：WO2021133770A1

公开(公告)日：2021-07-01

申请号：PCT/US2020/066549

申请日：2020-12-22

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： YANG, Xiangyuan ,  GEORGE, Wayne N. ,  GATTI LAFRANCONI, Pietro ,  TEO, Yin Nah ,  BROWN, Andrew, A. ,  BACIGALUPO, Maria, Rogert ,  BRUSTAD, Eric ,  BOHRA, Hassan ,  WANG, Clifford

IPC: C12Q1/6806 ,  C12Q1/6869 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00608 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6874 ,  C12Q2565/513

Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from an asymmetrical acrylamide polymer one or a dendrimer including lysyl constitutional repeating units, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.

公开(公告)号：WO2023075852A1

公开(公告)日：2023-05-04

申请号：PCT/US2022/028324

申请日：2022-05-09

Applicant: ILLUMINA, INC.

Inventor： FISHER, Jeffrey S. ,  FLANNERY, Anthony ,  HONG, Sahngki ,  KODIRA CARIAPPA, Brinda ,  KRAFT, Lewis J. ,  MATHER, Brian D.

IPC: B01J19/00 ,  B01L3/00 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L3/502707 ,  G03F7/0002 ,  G03F7/038 ,  G03F7/039

Abstract: A metal film is formed over a resin layer including a plurality of multi-depth depressions (MDP) separated by interstitial regions, each MDP including a deep portion and an adjacent shallow portion. A sacrificial layer is formed over the metal film. The sacrificial layer and metal film are sequentially dry etched to expose a resin layer surface at the shallow portion and interstitial regions. Resin layer portions are removed i) at the shallow portion to form a depression region having a surface directly adjacent to a surface at the deep portion and ii) at the interstitial regions to form new interstitial regions surrounding the deep portion and the depression region. First functionalized layer is deposited over the metal film, depression region, and new interstitial regions. The metal film is removed from the deep portion. Second functionalized layer is deposited over the surface at the deep portion. New interstitial regions are polished.

公开(公告)号：WO2022271689A1

公开(公告)日：2022-12-29

申请号：PCT/US2022/034330

申请日：2022-06-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SOFTWARE, INC.

Inventor： VIECELI, John S. ,  HAN, Hui ,  LANGLOIS, Robert ,  LU, Bo

IPC: B01J19/00 ,  B01L3/00 ,  G01N21/64 ,  B01J19/0046 ,  B01J2219/00554 ,  B01J2219/0056 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L2300/028 ,  B01L2300/0877 ,  B01L3/5085 ,  G01N15/1404 ,  G01N15/1434 ,  G01N2021/6482 ,  G01N21/05 ,  G01N21/6452 ,  G06T2207/30148 ,  G06T2207/30204 ,  G06T3/0068 ,  G06T7/0006 ,  G06T7/001 ,  G06T7/30

Abstract: Registration of a patterned flow cell may utilize fiducials comprising sets or groupings of features (e.g., sites, sample wells, nanowells) having known locations and in which the placement of the features is not in accordance with a periodic pattern or is otherwise distinguishable from the periodic pattern of sites present in non-fiducial regions of the flow cell substrate. In certain embodiments the positioning of the sites that are part of the fiducial represent a break or discontinuity in the periodic pattern of sites that are otherwise present on the surface of a patterned flow cell.

公开(公告)号：WO2022265994A1

公开(公告)日：2022-12-22

申请号：PCT/US2022/033257

申请日：2022-06-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： MA, Xiaoyu ,  XI, Weixian ,  FISHER, Jeffrey S. ,  SHEN, Fei ,  GEORGE, Wayne N. ,  MATHER, Brian D.

IPC: B01J19/00 ,  C08F8/00 ,  C12Q1/686 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00722 ,  B01J2219/00725 ,  C08K5/54 ,  C12Q1/6874

Abstract: Embodiments of the present application relate to substrate comprising a surface-bound azido functionalized organosilane wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer. Methods of preparing such substrate surface for sequencing applications are also disclosed.

公开(公告)号：WO2022256225A2

公开(公告)日：2022-12-08

申请号：PCT/US2022/031125

申请日：2022-05-26

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： KODIRA CARIAPPA, Brinda ,  GEORGE, Wayne N. ,  HONG, Sahngki ,  KRAFT, Lewis J.

IPC: B01J19/00 ,  G03F7/00 ,  G03F7/20 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00722 ,  G03F7/0035 ,  G03F7/0037 ,  G03F7/0382 ,  G03F7/2004 ,  G03F7/2014 ,  G03F7/2016 ,  G03F7/2022

Abstract: In an example of a method for making a flow cell, a light sensitive material is deposited over a resin layer including depressions separated by interstitial regions, wherein the depressions overlie a first resin portion having a first thickness and the interstitial regions overlie a second resin portion having a second thickness that is greater than the first thickness. A predetermined ultraviolet light dosage that is based on the first and second thicknesses is directed through the resin layer, whereby the light sensitive material overlying the depressions is exposed to ultraviolet light and the second resin portion absorbs the ultraviolet light, thereby defining an altered light sensitive material at a first predetermined region over the resin layer. The altered light sensitive material is utilized to generate a functionalized layer at the first predetermined region or at a second predetermined region over the resin layer.

公开(公告)号：WO2022072275A1

公开(公告)日：2022-04-07

申请号：PCT/US2021/052183

申请日：2021-09-27

Applicant: ILLUMINA, INC.

Inventor： HONG, Sahngki ,  KRAFT, Lewis J.

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  G01N21/05 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00608 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00722 ,  G01N2021/7763 ,  G03F7/0382 ,  G03F7/0392 ,  G03F7/2002 ,  G03F7/2043 ,  G03F7/343 ,  G03F7/38

Abstract: An example flow cell includes a multi-layer stack including a transparent base support; a patterned sacrificial layer over the transparent base support; and a transparent layer over the patterned sacrificial layer. The flow cell further includes first and second functionalized layers over different portions of the transparent layer, wherein at least one of the first and second functionalized layers aligns with a pattern of the patterned sacrificial layer; and first and second primer sets respectively attached to the first and second functionalized layer.