公开(公告)号：WO2022076581A2

公开(公告)日：2022-04-14

申请号：PCT/US2021/053801

申请日：2021-10-06

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： O'BOYLE, Duncan A. ,  GRIFFITH, Linda ,  TRUMPER, David

IPC: C12M3/06 ,  C12M1/34 ,  C12M1/00 ,  B01L3/00 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L9/50 ,  B01L9/527 ,  B29C65/16 ,  C12M23/16 ,  C12M29/00 ,  C12M41/40 ,  F16K99/00

Abstract: Materials and methods of making have been developed for mass production of thermoplastic microfluidic chips. An elastomer diaphragm with a stress relieving feature can be used in microfluidic valves, pump diaphragms, and diaphragm micropumps. An optimized pump chamber design for complete fluid displacement and chamber geometry are provided. Microfluidic pressure regulators use a pneumatically actuated elastic membrane in a back-pressure regulator configuration. Microfluidic accumulators store pressurized fluid in a microfluidic chip. Removable caps for cell culture and a quick release top are described. Methods to incorporate hydrogels and ECM scaffolds have been developed. Electro pneumatic manifolds connect and control of multiple microfluidic devices vertically or on a rotary mechanism.

公开(公告)号：WO2021155221A2

公开(公告)日：2021-08-05

申请号：PCT/US2021/015809

申请日：2021-01-29

Applicant: WESTERN CONNECTICUT HEALTH NETWORK, INC.

Inventor： ARRIGO, Lisa M. ,  CAMPBELL, Jennifer N. ,  FIEDLER, Paul N. ,  HAMMOND-MCKIBBEN, Denise

IPC: B01L3/02 ,  B01L3/00 ,  A61B10/00 ,  A61F13/38 ,  C12M1/30 ,  G01N1/02 ,  D01F8/14 ,  A61B5/15 ,  B01L2200/025 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2300/0845 ,  B01L2300/0861 ,  B01L2300/12 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/088 ,  B01L3/022 ,  B01L3/5029 ,  C12M33/00

Abstract: The present invention relates to devices and methods for collecting liquid samples such as for removing a target liquid sample fraction from a larger liquid sample., e.g., from a biological sample. These devices and methods are particularly useful for rapidly and cost- effectively removing the buffy coat layer from an anticoagulated blood sample. These devices and methods have the advantage that the removal can be made on blood sample processed by ordinary centrifugation and do not require more complicated or costly processing techniques such as density gradient centrifugation.

公开(公告)号：WO2020154484A1

公开(公告)日：2020-07-30

申请号：PCT/US2020/014758

申请日：2020-01-23

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： XU, Qihua ,  WEIDEMAIER, Kristin ,  SALOMON, John, E. ,  LASTOVICH, Alexander, G. ,  FALLOWS, Eric, A. ,  CONNELL, Sean ,  HERR, Joshua ,  WOLFGANG, Meghan ,  BRASCH, Michael, A. ,  MOORE, Richard, L. ,  SEBBA, David, S. ,  CLAVIJO, Cristian ,  NG, Shirley ,  ABBOT, Richard ,  PAPP, Alexander, Adam ,  FU, Henry, Li-wei ,  AUSTIN, Caitlin, Marie ,  DOWLING, Sean, Patrick ,  JOYCE, Owen, Lewis ,  KIPLINGER, Michael, L. ,  CARPENTER, William, Kevin

IPC: B01L3/00 ,  G01N1/40 ,  A61M5/165 ,  B01D61/18 ,  C12M1/00 ,  A61M2005/1657 ,  B01D2311/25 ,  B01D2315/10 ,  B01D61/142 ,  B01D63/087 ,  B01D67/0032 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0478 ,  B01L2400/049 ,  B01L3/502753 ,  B01L3/502761 ,  C12M33/14 ,  C12M47/04 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088

Abstract: Methods and apparatus provide filtration for concentrating analytes, such as bacteria or exosomes, of a biological sample, such as blood or urine. The technology may employ membrane devices that implement one or more tangential flow filtration processes such as in stages. An example membrane device may typically include a membrane having sides and ends. The membrane may selectively permit constituent(s) of the sample to pass through while retaining other constituents at one side. An input chamber of the device may include an inlet near one end and an outlet near the other end, and that may permit a tangential flow of the sample along the first side surface, and a trans-membrane passing of constituent(s). An output chamber of the device may be configured at the second side surface to receive the passing constituents. Such devices may be provided in a kit to facilitate targeting of a desired biological analyte concentration.

公开(公告)号：WO2023275831A1

公开(公告)日：2023-01-05

申请号：PCT/IB2022/056132

申请日：2022-06-30

Applicant: CREGANNA UNLIMITED COMPANY

Inventor： WRIGHT, David W. ,  MITCHELL, Fred ,  CASTILLO, Benjamin A.

IPC: B01L3/00 ,  C12Q1/00 ,  G01N27/327 ,  B01F33/30 ,  G01N35/00 ,  B01F33/304 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0683 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/523 ,  C12Q1/006

Abstract: A disposable diagnostic device (10) includes a body (12) having a first channel (14) and a second channel (16) spaced from the first channel. A blister (18) is operably fixed to the body and encloses a chamber (20) which is configured in a hermetically sealed-off relation from the first and second channels when the device is in a non-activated first state and is in open communication with at least one of the first and second channels when the device is in an activated second state. A reactant (22) and an inert gas (24) are disposed in the chamber such that the inert gas protects the reactant from being exposed to contaminants when the device is in said non-activated first state. A method of constructing a disposable diagnostic device is also disclosed.

公开(公告)号：WO2022032094A1

公开(公告)日：2022-02-10

申请号：PCT/US2021/044940

申请日：2021-08-06

Applicant: OVERTURE LIFE, INC.

Inventor： MUNNE, Santiago ,  HORCAJADAS ALMANSA, José Antonio ,  MATTHYS, Lionel ,  SALES FIDALGO, Joana Alexandra ,  CARASA RUIZ, Pablo ,  MARTIN VILLALBA, Tamara ,  CANCIO VILLALONGA, David ,  GARCIA ALONSO, Daniel ,  DOSDA MUNUERA, Cristina ,  ALVAREZ ARGUELLES, Sara

IPC: A61B17/425 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/14 ,  B01L2400/049 ,  B01L2400/06 ,  B01L3/502715

Abstract: Disclosed herein are microfluidic devices and methods to deliver concentration gradients to biological material such as oocytes and embryos for the purpose of cryopreparation, cryopreservation, or thawing. Cryopreservation methods, such as vitrification, involve the use of cryoprotectants to reduce formation of damaging ice crystals in cells during freezing. Microfluidic devices and methods described herein improve cell viability and efficiency during handling and cryopreservation of biological materials.

公开(公告)号：WO2023007224A1

公开(公告)日：2023-02-02

申请号：PCT/IB2021/056899

申请日：2021-07-29

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： DAINESE, Riccardo ,  ALPERN, Daniel ,  DEPLANCKE, Bart ,  RAGIOS, Konstantinos

IPC: B01L3/00 ,  B67C11/00 ,  B01L2200/026 ,  B01L2200/0631 ,  B01L2300/0809 ,  B01L2300/0829 ,  B01L2400/049 ,  B01L3/502 ,  B01L3/563

Abstract: The present invention concerns a biological material pooling device for biological or biomolecule purification comprising a receptacle including a first orifice comprising ordefining a rectangular opening, the receptacle being configured to receive at least one liquid through the first orifice, a second orifice located opposite the first orifice; and at least one side wall extending between the first orifice and the second orifice. The at least one side wall extends between the first orifice and the second orifice to define a tapered cavity or a cavity of decreasing cross-sectional area between the first orifice and the second orifice to guide and remove the at least one liquid out of the biological material pooling device.

公开(公告)号：WO2021198296A1

公开(公告)日：2021-10-07

申请号：PCT/EP2021/058363

申请日：2021-03-30

Applicant: OSLER DIAGNOSTICS LIMITED

Inventor： LILLIS, Barry ,  GREEN, Steven

IPC: B01L3/00 ,  G01N33/00 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L3/50273 ,  B01L3/502738 ,  G01N35/1016 ,  G01N35/1065

Abstract: The present disclosure relates to a liquid handling device, methods of operating a liquid handling device, a method of performing a diagnostic test, a computer program and a system. In one aspect, a liquid handling device may comprise a main chamber; a sample chamber for receiving a sample, such as a saliva, blood or urine sample; one or more measurement chambers for performing measurements on the sample; a variable pressure source conduit for connecting the main chamber to a variable pressure source; a sample chamber conduit which fluidically connects the sample chamber to the main chamber; a sample chamber conduit valve for opening and closing the sample chamber conduit; a respective measurement chamber conduit for each measurement chamber, wherein each respective measurement chamber conduit fluidically connects the respective measurement chamber to the main chamber; and a respective measurement chamber conduit valve for opening and closing each respective measurement chamber conduit.

公开(公告)号：WO2021026249A8

公开(公告)日：2021-02-11

申请号：PCT/US2020/045030

申请日：2020-08-05

Applicant: BD KIESTRA B.V.

Inventor： FEIJEN, Franciscus, Hermannus ,  ARMSTRONG, Robert, Edward ,  LENTZ, Ammon, David ,  POHL, Brent, Ronald

IPC: C12M1/00 ,  C12M1/26 ,  B01L3/02 ,  B01L2200/0684 ,  B01L2300/042 ,  B01L2300/0672 ,  B01L2400/049 ,  B01L3/5635 ,  C12M33/04

Abstract: A multi-port disposable device that can both vent a sealed culture vessel and draw a sample therefrom into a collection vessel. The multi-port disposable device has a first port configured to receive the top portion of a culture vessel and attach thereto. The multi-port disposable device has a second port configured to receive a sample collection vessel. The multi-port disposable device has first and second needles. The first needle has a cannula configured to penetrate a septum or cap of the culture vessel. Optionally, the cannula terminates in a layer of foam that carries a microbial agent such that any vapor that is transmitted from the culture vessel through the cannula when venting the sealed culture vessel is absorbed by the foam. Optionally, the ports of the disposable device are configured as sleeves. The sleeve of the first port receives a portion of the culture vessel and the sleeve of the second port receives the collection vessel. The second needle has a cannula that penetrates both the septum or cap of the culture vessel and the septum or cap of the collection vessel, when the culture vessel and the collection vessel are brought into the device, thereby providing for fluid communication between the culture vessel and the collection vessel. The second cannula provides the flow pathway for delivering sample from the culture vessel to the collection vessel.