中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

16 records (took 0.008 seconds)

    CLONING AND EXPRESSION OF BIOLOGICALLY ACTIVE ALPHA-N-ACETYLGALACTOSAMINIDASE
    2.
    发明申请
    CLONING AND EXPRESSION OF BIOLOGICALLY ACTIVE ALPHA-N-ACETYLGALACTOSAMINIDASE 审中-公开
    生物活性ALPHA-N-乙酰胆碱酯酶的克隆与表达

    公开(公告)号：WO1992007936A1

    公开(公告)日：1992-05-14

    申请号：PCT/US1991007872

    申请日：1991-10-23

    Applicant: THE MOUNT SINAI SCHOOL OF MEDICINE OF THE CITY ...

    Inventor： THE MOUNT SINAI SCHOOL OF MEDICINE OF THE CITY ... ,  DESNICK, Robert, J. ,  BISHOP, David, F. ,  IOANNOU, Yiannis, A. ,  WANG, Anne, M.

    IPC: C12N09/26

    CPC classification number: C12Y302/01049 ,  A61K38/00 ,  C12N9/2465

    Abstract: The present invention involves the production of human alpha -GalNAc by cloning and expressing the alpha -GalNAc coding sequence in eukaryotic host cell expressions systems. The eukaryotic expression systems, and in particular the mammalian host cell expression systems described herein provide for the appropriate co-translational and post-translation modifications required for proper processing, e.g., glycosylation, phosphorylation, etc. and sorting of the expression product so that an active enzyme is produced. The alpha -GalNAc produced in accordance with the invention may be used in the treatment of Schindler disease or for the hydrolysis of alpha -N-acetylgalactosaminyl moieties in various glycoconjugates.

    Abstract translation: 本发明涉及通过在真核宿主细胞表达系统中克隆和表达α-GalNAc编码序列来生产人α-GalNAc。 真核表达系统，特别是本文所述的哺乳动物宿主细胞表达系统提供适当的协同翻译和翻译后修饰，以进行适当的加工（例如糖基化，磷酸化等）和表达产物的分选，使得 生产活性酶。 根据本发明产生的α-GalNAc可用于治疗辛德勒病或用于各种糖缀合物中α-N-乙酰半乳糖胺基部分的水解。

    PROTOPECTINASE
    3.
    发明申请
    PROTOPECTINASE 审中-公开
    原果胶酶

    公开(公告)号：WO1998006832A1

    公开(公告)日：1998-02-19

    申请号：PCT/JP1997002794

    申请日：1997-08-08

    Applicant: SAKAI, Takuo

    IPC: C12N09/26

    CPC classification number: C12N9/2408

    Abstract: A novel protopectinase; a DNA encoding this protopectinase; an expression vector containing this DNA; host cells containing this expression vector; and a process for producing the above-mentioned protopectinase.

    Abstract translation: 一种新颖的原花青素; 编码该蛋白酶的DNA; 含有该DNA的表达载体; 含有该表达载体的宿主细胞; 以及用于制备上述原花色素酶的方法。

    A NEW GLUCAN LYASE PRODUCING 1,5-ANHYDROFRUCTOSE
    7.
    发明申请
    A NEW GLUCAN LYASE PRODUCING 1,5-ANHYDROFRUCTOSE 审中-公开
    一种新的GLUCAN LYASE生产1,5-ANHYDROFRUCTOSE

    公开(公告)号：WO1994009122A1

    公开(公告)日：1994-04-28

    申请号：PCT/SE1993000854

    申请日：1993-10-19

    Applicant: ALGATECH AB ,  KENNE, Lennart ,  PEDERSÉN, Marianne ,  YU, Shukun

    Inventor： ALGATECH AB

    IPC: C12N09/26

    CPC classification number: C12P19/02 ,  C07H3/10 ,  C12N9/88

    Abstract: An enzyme, exo- alpha -1,4-glucan lyase, capable of successively cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan is disclosed. The enzyme is e.g. selected from the group consisting of alpha -1,4-glucan lyase isolated from an alga and functional derivatives and analogs derived from a nucleotide sequence related to an alga alpha -1,4-glucan lyase. Further, a method of enzymatically cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan, and the degradation product 1,5-anhydrofructose for use as an oxygen radical scavenger or anti-oxidant and/or as a sugar substitute, are disclosed. Additionally, a method of producing an enzyme capable of successively cleaving the terminal alpha -1,4-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan; an antibody which binds to the amino acid sequence of the enzyme, and a DNA or RNA probe which recognizes a nucleotide sequence coding for the enzyme, are described.

    Abstract translation: 公开了能够从α-1,4-葡聚糖的非还原性末端连续切割末端α-1,4-D-糖苷键的酶，外-α-1,4-葡聚糖裂解酶。 酶是例如 选自由藻类分离的α-1,4-葡聚糖裂解酶和功能性衍生物和衍生自与藻类α-1,4-葡聚糖裂解酶相关的核苷酸序列的类似物。 此外，从α-1,4-葡聚糖的非还原末端酶切地切割末端α-1,4- D - 糖苷键的方法，以及用作氧自由基的降解产物1,5-脱水果糖 清除剂或抗氧化剂和/或糖代用品。 另外，生产能够从α-1,4-葡聚糖的非还原性末端依次切断末端α-1,4-糖苷键的酶的方法; 描述了结合酶的氨基酸序列的抗体和识别编码该酶的核苷酸序列的DNA或RNA探针。

    CYCLOMALTODEXTRIN GLUCANOTRANSFERASE VARIANTS
    8.
    发明申请
    CYCLOMALTODEXTRIN GLUCANOTRANSFERASE VARIANTS 审中-公开
    CYCLOMALTODEXTRIN葡萄糖转运酶变体

    公开(公告)号：WO1996033267A1

    公开(公告)日：1996-10-24

    申请号：PCT/DK1996000179

    申请日：1996-04-22

    Applicant: NOVO NORDISK A/S ,  DIJKHUIZEN, Lubbert ,  DIJKSTRA, Bauke, W. ,  ANDERSEN, Carsten ,  VON DER OSTEN, Claus

    Inventor： NOVO NORDISK A/S

    IPC: C12N09/26

    CPC classification number: C12N9/1074 ,  C12P19/18 ,  C12Y204/01019

    Abstract: The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically, the invention relates to a maethod of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.

    Abstract translation: 本发明涉及环糊精葡聚糖转移酶的变体。 更具体地，本发明涉及通过取代，插入和/或缺失一个或多个氨基酸残基来修饰前体CGT酶的底物结合和/或产物选择性的方法和衍生自前体CGT酶的CGTase变体（ s），哪个氨基酸残基保持靠近底物的位置。 此外，本发明涉及编码CGTase变体，表达载体，宿主细胞的DNA构建体和产生本发明的CGTase变体的方法。

Patent Agency Ranking