公开(公告)号：WO1997020918A1

公开(公告)日：1997-06-12

申请号：PCT/US1996019457

申请日：1996-12-06

Applicant: RECOMBINANT BIOCATALYSIS, INC.

Inventor： RECOMBINANT BIOCATALYSIS, INC. ,  SHORT, Jay, M.

IPC: C12N09/00

CPC classification number: C12N15/1086 ,  C12N9/16 ,  C12N9/2434 ,  C12N15/1058 ,  C12Q1/6811 ,  C12Y301/03001 ,  C12Y302/01021

Abstract: Disclosed are processes to identify desired enzymatic activity from a pool of DNA collected from one or more organisms or a DNA subjected to random directed mutagenesis. The methods involve the generation of DNA library in a host cell and screening for the desired activity. The process can be applied to develop thermally stable proteins having improved enzymatic activity at lower temperature.

Abstract translation: 公开的是从从一种或多种生物体或经受随机定向诱变的DNA收集的DNA池中鉴定所需酶活性的方法。 所述方法涉及在宿主细胞中产生DNA文库并筛选所需的活性。 该方法可用于开发在较低温度下具有改善的酶活性的热稳定蛋白质。

公开(公告)号：WO1997004077A1

公开(公告)日：1997-02-06

申请号：PCT/US1996011854

申请日：1996-07-17

Applicant: RECOMBINANT BIOCATALYSIS, INC. ,  SHORT, Jay, M. ,  MARRS, Barry ,  STEIN, Jeffrey, L.

Inventor： RECOMBINANT BIOCATALYSIS, INC.

IPC: C12N09/00

CPC classification number: C12N15/1086 ,  C12N15/1034 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/52 ,  C12Q1/00

Abstract: Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms. Also disclosed is a process for identifying clones of a recombinant library which express a protein with a desired activity by screening a library of expression clones randomly produced from DNA of at least one microorganism, said screening being effected on expression products of said clones to thereby identify clones which express a protein with a desired activity. Also disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein activity by screening for a specified protein activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein activity.

Abstract translation: 重组酶文库和试剂盒，其中多个酶各自以不同的物理和/或化学特征为特征，并通过共同特征分类。 通过筛选由各种微生物产生的DNA文库表达的重组酶来确定特征。 还公开了通过筛选由至少一种微生物的DNA随机产生的表达克隆文库来鉴定表达具有所需活性的蛋白质的重组文库的克隆的方法，所述筛选是对所述克隆的表达产物进行鉴定，从而鉴定 表达具有所需活性的蛋白质的克隆。 还公开了通过筛选通过（i）从源自至少一个未培养微生物的DNA群体中回收DNA的克隆文库中筛选特定蛋白质活性来筛选具有来自未培养的微生物的具有DNA的克隆的克隆的方法，以获得特定的蛋白质活性; 和（ii）用回收的DNA转化宿主以产生筛选特定蛋白质活性的克隆文库。

公开(公告)号：WO1998001581A1

公开(公告)日：1998-01-15

申请号：PCT/US1997012239

申请日：1997-07-09

Applicant: RECOMBINANT BIOCATALYSIS, INC. ,  SHORT, Jay, M.

Inventor： RECOMBINANT BIOCATALYSIS, INC.

IPC: C12Q01/68

CPC classification number: C12Q1/6811 ,  C12N15/1027

Abstract: Disclosed is a process of performing "Sexual" PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.

Abstract translation: 公开了进行“性”PCR的方法，其包括通过中断或阻断合成或扩增过程产生随机多核苷酸，以显示或停止至少一种多核苷酸的合成或扩增，任选地扩增多核苷酸，以及重新退火多核苷酸以产生随机突变体 多核苷酸。 还提供了载体和表达载体，包括这种突变多核苷酸，由突变多核苷酸表达的多肽和用于产生随机突变多肽的方法。

公开(公告)号：WO1997048794A1

公开(公告)日：1997-12-24

申请号：PCT/US1997009319

申请日：1997-06-17

Applicant: RECOMBINANT BIOCATALYSIS, INC.

Inventor： RECOMBINANT BIOCATALYSIS, INC. ,  MURPHY, Dennis ,  REID, John, C. ,  ROBERTSON, Dan

IPC: C12N09/80

CPC classification number: C12P21/06 ,  C12N9/80

Abstract: A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or "natural" enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.

Abstract translation: 纯化的热稳定性酶来源于弓形虫Thermococcus GU5L5。 该酶的分子量约为68.5千道尔顿，具有纤维素酶活性。 该酶可以由天然的或重组的宿主细胞产生，并且可用于从肽或肽模拟合成中的肽的N-末端去除精氨酸，苯丙氨酸或甲硫氨酸氨基酸。 该酶对氨基酸衍生物的L或“天然”对映异构体是选择性的，因此可用于制备光学活性化合物。 这些反应可以在化学上更具反应性的酯官能团的存在下进行，这是非常难以用非酶法实现的步骤。

公开(公告)号：WO1997044361A1

公开(公告)日：1997-11-27

申请号：PCT/US1997008793

申请日：1997-05-22

Applicant: RECOMBINANT BIOCATALYSIS, INC. ,  LAM, David, E. ,  MATHUR, Eric, J.

Inventor： RECOMBINANT BIOCATALYSIS, INC.

IPC: C07K16/40

CPC classification number: C12N9/2437 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01021

Abstract: The invention provides a purified thermostable enzyme derived from the archael bacterium AEPII1a. The enzyme has a molecular weight of about 60.9 kilodaltons and has cellulase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of cellulose where desired. Also included are other endoglucanases having homology to SEQ ID NO:2.

Abstract translation: 本发明提供了源自阿尔德细菌AEPII1a的纯化的热稳定酶。 该酶具有约60.9千道尔顿的分子量并具有纤维素酶活性（SEQ ID NO：2）。 该酶可以由天然的或重组的宿主细胞产生，并且可以用于有助于纤维素的消化。 还包括与SEQ ID NO：2具有同源性的其它内切葡聚糖酶。

公开(公告)号：WO1997030160A1

公开(公告)日：1997-08-21

申请号：PCT/US1997002039

申请日：1997-02-11

Applicant: RECOMBINANT BIOCATALYSIS, INC. ,  ROBERTSON, Dan, E. ,  MURPHY, Dennis ,  REID, John ,  MAFFIA, Anthony, M. ,  LINK, Steven ,  SWANSON, Ronald, V. ,  WARREN, Patrick, V. ,  KOSMOTKA, Anna ,  CALLEN, Walter

Inventor： RECOMBINANT BIOCATALYSIS, INC.

IPC: C12N15/55

CPC classification number: C12N9/16 ,  C12N9/18 ,  C12P13/04

Abstract: Esterase enzymes derived from various Staphylothermus, Pyrodictium, Archaeoglobus, Aquifex, M11TL, Thermococcus, Teredinibacter and Sulfolobus organisms are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the pharmaceutical, agricultural and other industries.

Abstract translation: 公开了衍生自各种葡萄球菌，火炬蒿，古细血管，Aquifex，M11TL，Thermococcus，Teredinibacter和Sulfolobus生物体的酯酶。 酶由天然或重组宿主细胞产生，可​​用于制药，农业和其他工业。

公开(公告)号：WO1997025417A1

公开(公告)日：1997-07-17

申请号：PCT/US1997000092

申请日：1997-01-10

Applicant: RECOMBINANT BIOCATALYSIS, INC.

Inventor： RECOMBINANT BIOCATALYSIS, INC. ,  BYLINA, Edward, J. ,  SWANSON, Ronald, V. ,  MATHUR, Eric, J. ,  LAM, David, E.

IPC: C12N09/26

CPC classification number: C12N9/2457 ,  C12N9/2402 ,  C12N9/2451 ,  C12N9/2468 ,  C12N9/2471 ,  C12N9/2494 ,  C12Y302/01022 ,  C12Y302/01023 ,  C12Y302/01025 ,  C12Y302/01041 ,  C12Y302/01078 ,  C12Y302/0115

Abstract: A thermostable glycosidase enzymes derived from various thermococcus, staphylothermus and pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.

Abstract translation: 公开了衍生自各种热球菌，葡萄球菌和热生球菌的热稳定性糖苷酶。 酶由天然或重组宿主细胞产生，可​​用于食品加工业，制药工业，纺织工业，洗涤剂行业和烘焙行业。

公开(公告)号：WO1998000526A1

公开(公告)日：1998-01-08

申请号：PCT/US1997016513

申请日：1997-07-03

Applicant: RECOMBINANT BIOCATALYSIS, INC.

Inventor： RECOMBINANT BIOCATALYSIS, INC. ,  ROBERTSON, Dan, E. ,  SANYAL, Indrajit ,  ADHIKARY, Robert, S.

IPC: C12N09/08

CPC classification number: C12N9/0065 ,  A01K2217/05 ,  C12Q1/30

Abstract: Catalase enzymes derived from bacterial for the genera Alcaligenes (Delaya) and MicroscUla are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized to destroy or detect hydrogen peroxide, e.g., in production of glyoxylic acid and in glucose sensors, and in processes where hydrogen peroxide is used as a bleaching or antibacterial agent, e.g., in contact lens cleaning, in bleaching steps in pulp and paper preparation and in the pasteurization of dairy products.

Abstract translation: 公开了来自产碱杆菌属（Delaya）和MicroscUla属的细菌的过氧化氢酶。 酶由天然或重组宿主细胞产生，可​​用于破坏或检测过氧化氢，例如在生产乙醛酸和葡萄糖传感器时，以及在过氧化氢用作漂白剂或抗菌剂的过程中， 在隐形眼镜清洁中，在纸浆和纸制备中的漂白步骤和乳制品的巴氏消毒中。

公开(公告)号：WO1997048717A1

公开(公告)日：1997-12-24

申请号：PCT/US1997010748

申请日：1997-06-18

Applicant: RECOMBINANT BIOCATALYSIS, INC.

Inventor： RECOMBINANT BIOCATALYSIS, INC. ,  SHORT, Jay, M. ,  MATHUR, Eric, J.

IPC: C07H21/02

CPC classification number: C12N15/1093 ,  C12N15/1096 ,  C12Q1/6811

Abstract: Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

Abstract translation: 公开了通过（a）从环境样品中分离基因组DNA群体从环境样品形成归一化基因组DNA文库的方法; （b）分析如此分离的基因组DNA群体的复杂性; （c）至少一个（i）扩增如此分离的DNA群体的拷贝数，和（ii）回收具有所需特征的分离的基因组DNA的一部分; 和（d）使基因组DNA群体内的各种DNA的表达正常化，从而形成来自环境样品的基因组DNA的归一化文库。 还公开了通过该方法由环境样品形成的归一化的基因组DNA文库。

公开(公告)号：WO1997048416A1

公开(公告)日：1997-12-24

申请号：PCT/US1997010784

申请日：1997-06-19

Applicant: RECOMBINANT BIOCATALYSIS, INC. ,  MATHUR, Eric, J. ,  LEE, Edd ,  BYLINA, Edward

Inventor： RECOMBINANT BIOCATALYSIS, INC.

IPC: A61K38/46

CPC classification number: C12N9/16

Abstract: Thermostable alkaline phosphatase enzymes derived from bacteria from the genus Ammonifex, Aquifex, Archaeoglobus, Desulfurococcus, Methanococcus, Thermotogales, Pyrolobus, Pyrococcus, and Thermococcus organisms are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the pharmaceutical, food, detergent, and baking industry.

Abstract translation: 公开了源自Ammonifex，Aquifex，Archaeoglobus，Desulfurococcus，Methanococcus，Thermotogales，Pyrolobus，Pyrococcus和Thermococcus生物的细菌的热稳定性碱性磷酸酶。 酶由天然或重组宿主细胞产生，可​​用于制药，食品，洗涤剂和烘焙工业。