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公开(公告)号：WO2023082011A1
公开(公告)日：2023-05-19
申请号：PCT/CA2022/051668
申请日：2022-11-11
发明人： VEYRIER, Frédéric , CHENAL, Martin
摘要： The present application relates to endonucleases specific for single-stranded nucleic acid molecules. These endonucleases having a length of 60 to 150 amino acids or less and comprises a single GIY-YIG domain. The present application also relates to compositions and cells comprising such endonucleases as well as to methods of cleaving single-stranded nucleic acid molecules comprising a nucleotide sequence recognized by the endonucleases.
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公开(公告)号：WO2023273959A1
公开(公告)日：2023-01-05
申请号：PCT/CN2022/100139
申请日：2022-06-21
申请人： 康盈红莓（中山）生物科技有限公司
摘要： 提供了一种腺苷参与的NMN的全酶法合成方法，所述腺苷参与的NMN的全酶法合成方法包括同一反应体系下的步骤：(A)腺苷、磷酸盐以及可被酵母细胞代谢的糖类在酵母细胞的催化作用下反应生成ATP；(B)酶促反应生产NMN的步骤，包括在NAMPT作用下，烟酰胺、PRPP以及ATP反应生成NMN、ADP以及磷酸盐的步骤；如此以将ATP的生成(再生)、NMN的合成及ATP的利用等一系列反应统一在一个反应体系内进行，即可完成NMN的高效合成。
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公开(公告)号：WO2022251242A1
公开(公告)日：2022-12-01
申请号：PCT/US2022/030760
申请日：2022-05-24
发明人： TOTA, Ember, M. , DEVARAJ, Neal
IPC分类号： C07D487/02 , C07D487/04 , C12P19/34 , C12Q1/48 , C12P19/30
摘要： Provided herein are, inter alia, compositions and methods for modifying a DNA molecule. The methods include contacting a DNA molecule with a transglycosyl ase enzyme and a Pre-queuosinel (preQi) analog or derivative, wherein a guanine nucleobase within a hairpin structure in the DNA molecule is exchanged for the preQi analog or derivative.
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公开(公告)号：WO2022002918A1
公开(公告)日：2022-01-06
申请号：PCT/EP2021/067816
申请日：2021-06-29
申请人： C-LECTA GMBH
发明人： VOGEL, Andreas , FELLER, Claudia , BAYER, Christopher David , HOFFMANN, Gregor , SCHÖNERT, Stefan
摘要： The invention relates to a process for the preparation of a glycosylated organic compound by in vitro glycosylation of an organic compound having a nucleophilic group with a saccharide under catalysis of a Leloir glycosyltrans-ferase system comprising at least a first glycosyl transferase and a second glycosyl transferase. The invention further relates to a composition comprising a glycosylated organic compound that is obtainable by the process according to the invention.
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公开(公告)号：WO2021253362A1
公开(公告)日：2021-12-23
申请号：PCT/CN2020/096908
申请日：2020-06-19
申请人： 邦泰生物工程（深圳）有限公司
IPC分类号： C07H19/048 , C07H1/00 , C12P19/30
摘要： 一种以烟酰胺为原料制备烟酰胺单核苷酸的方法，包括：在乙腈、二氯甲烷、1,2-二氯乙烷或者液态二氧化硫溶剂中，用三氟甲磺酸三甲基硅酯或四氯化锡催化烟酰胺和四乙酰核糖进行反应，调节pH值为3～5，加入甲醇钠溶液，于-15～5℃进行反应，调节pH值为3～5，用膜浓缩设备进行微滤和纳滤处理，得烟酰胺核糖溶液；在Mg离子、ATP和缓冲液存在的环境中，用烟酰胺核糖激酶催化烟酰胺核糖溶液进行反应，即得烟酰胺单核苷酸。该方法减少了对烟酰胺核糖的精制步骤，工艺更精简、成本更低廉、耗时更短，并且相较于直接采用烟酰胺核糖精制固体的酶催化过程具有更快的反应速度、更低的酶用量等优点。
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公开(公告)号：WO2020058493A1
公开(公告)日：2020-03-26
申请号：PCT/EP2019/075371
申请日：2019-09-20
申请人： UNIVERSITEIT GENT
IPC分类号： C12P19/26 , C12P19/44 , C12P17/16 , C12P21/00 , C12P19/30 , C12P7/64 , C12P11/00 , C12P19/00 , C12N9/10
摘要： The present invention discloses a method to produce amino sugar (containing) products using metabolically engineered microorganisms wherein the conversion of UDP-N- acetylglucosamine to cell envelope precursors and molecules is reduced by altering the activity of enzymes involved in the synthesis of cell envelope precursors and molecules.
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公开(公告)号：WO2019051250A1
公开(公告)日：2019-03-14
申请号：PCT/US2018/049988
申请日：2018-09-07
CPC分类号： C12N9/1252 , C07H21/00 , C12N15/1068 , C12N15/1096 , C12N15/52 , C12P19/34 , C12Q1/6806 , C12Q1/6844 , C12Q2521/101 , C12Q2525/117 , C12Y207/07001
摘要： Methods for de novo synthesis of polynucleotides in which 3'-O-reversibly blocked nucleotides are attached to a solid support in the presence of an X family DNA polymerase and in the absence of a nucleic acid template.
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公开(公告)号：WO2019005901A1
公开(公告)日：2019-01-03
申请号：PCT/US2018/039658
申请日：2018-06-27
摘要： Compositions and method are therefore disclosed for treating asthma. In particular, disclosed a composition that contains one, two, or more cytidine diphosphate (CDP)-conjugated phospholipid precursors selected from the group consisting of CDP-choline, CDP-ethanolamine, and CDP-diacylglycerol in a pharmaceutically acceptable carrier for use in treating asthma.
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公开(公告)号：WO2017176541A1
公开(公告)日：2017-10-12
申请号：PCT/US2017/024939
申请日：2017-03-30
发明人： LEE, Henry H. , CHURCH, George M. , KALHOR, Reza
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2565/519 , C12Q2565/629
摘要： The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3' terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3' terminal nucleotide such that the selected nucleotide becomes a 3' terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
摘要翻译： 本公开提供了用于制造多核苷酸的方法，其中核苷酸的添加可以被物理地，化学地和/或酶学地控制。 所述方法包括在包含与其连接并具有3'末端核苷酸的引发序列的反应位点组合选定的核苷酸，阳离子，易错或模板独立的DNA聚合酶，其中反应试剂可以在允许共价加成的条件下 一个或多个选择的核苷酸与3'末端核苷酸连接，使得所选择的核苷酸变成3'末端核苷酸，并且重复该加入步骤直到形成多核苷酸。
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公开(公告)号：WO2017160788A3
公开(公告)日：2017-09-21
申请号：PCT/US2017/022232
申请日：2017-03-14
申请人： RGENE, INC.
发明人： ZHENG, Yu , HONG, Manqing
摘要： Provided herein are compositions, systems, and methods employing hyper-thermostable lysine-mutant ssDNA/RNA ligases that possesses both ssRNA ligase and ssDNA ligase activity. In certain embodiments, such hyper-thermostable lysine-mutant ssDNA/RNA ligases are used to ligate an first single stranded nucleic acid sequence with a 5' adenylated end to a second single stranded nucleic acid sequence (e.g., at a temperature of at least 75°C) to form a ligated nucleic acid sequence. In further embodiments, the ligated nucleic acid sequence is sequenced.
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