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公开(公告)号：WO2023273959A1
公开(公告)日：2023-01-05
申请号：PCT/CN2022/100139
申请日：2022-06-21
申请人： 康盈红莓（中山）生物科技有限公司
摘要： 提供了一种腺苷参与的NMN的全酶法合成方法，所述腺苷参与的NMN的全酶法合成方法包括同一反应体系下的步骤：(A)腺苷、磷酸盐以及可被酵母细胞代谢的糖类在酵母细胞的催化作用下反应生成ATP；(B)酶促反应生产NMN的步骤，包括在NAMPT作用下，烟酰胺、PRPP以及ATP反应生成NMN、ADP以及磷酸盐的步骤；如此以将ATP的生成(再生)、NMN的合成及ATP的利用等一系列反应统一在一个反应体系内进行，即可完成NMN的高效合成。
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3.发明申请
公开(公告)号：WO2022225100A1
公开(公告)日：2022-10-27
申请号：PCT/KR2021/009545
申请日：2021-07-23
申请人： 씨제이제일제당 (주)
摘要： 본 출원은 신규한 이중기능성 메틸렌테트라히드로폴레이트 탈수소효소/메테닐테트라하이드로폴레이트 사이클로하이드롤라아제 변이체, 상기 변이체를 포함하는 코리네박테리움 스테이셔니스 균주 및 상기 균주를 이용한 XMP 또는 GMP 생산 방법에 관한 것이다.
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公开(公告)号：WO2022192865A1
公开(公告)日：2022-09-15
申请号：PCT/US2022/071020
申请日：2022-03-08
申请人： LANZATECH, INC.
发明人： GARG, Shivani , KOEPKE, Michael
IPC分类号： C12N15/52 , C12N15/74 , C12N9/10 , C12N9/02 , C12N9/16 , C12N1/20 , C12P7/06 , C12P7/18 , C12P7/6409 , C12N2800/101 , C12P19/32 , C12P7/04 , C12P7/16 , C12P7/52 , C12Y101/0133 , C12Y103/01009 , C12Y203/01009 , C12Y402/01017
摘要： Microorganisms are genetically engineered to produce various chemicals for industrial use. The microorganisms are carboxydotrophic acetogens. The microorganisms produce acetyl-CoA using the Wood-Ljungdahl Pathway for fixing CO/CO2. A reverse beta-oxidation pathway cycle from a microorganism that contains such a group of enzymes is introduced. Additionally, primers and extenders, and/or genes encoding for enzymes that generate primers and extenders may also be introduced. Product synthesis can be effected by improved promoters or enzyme designs that are catalytically more efficient. Similarly, product synthesis may also be improved by deleting competing reactions.
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公开(公告)号：WO2022154184A1
公开(公告)日：2022-07-21
申请号：PCT/KR2021/005028
申请日：2021-04-21
申请人： 씨제이제일제당 (주)
摘要： 본 출원은 신규한 셀레니드, 물 디키나제(Selenide, water dikinase) 변이체, 상기 변이체를 포함하는 코리네박테리움 스테이셔니스(Corynebacterium stationis) 균주 및 상기 균주를 이용한 IMP(5'-inosine monophosphate) 생산 방법에 관한 것이다.
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公开(公告)号：WO2022133223A1
公开(公告)日：2022-06-23
申请号：PCT/US2021/064049
申请日：2021-12-17
IPC分类号： C12M1/24 , C12M1/40 , C12N11/18 , B01L2200/028 , B01L2200/0631 , B01L2200/0689 , B01L2200/146 , B01L2200/147 , B01L2300/048 , B01L2300/0663 , B01L2300/0832 , B01L2300/163 , B01L2300/1894 , B01L3/00 , C12P19/32 , C12P7/40 , C12P7/42 , C12P9/00
摘要： The present invention features a versatile continuous manufacturing platform for cell-free chemical production.
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公开(公告)号：WO2022039250A1
公开(公告)日：2022-02-24
申请号：PCT/JP2021/030516
申请日：2021-08-20
摘要： ヒト人工多能性幹細胞の培養方法は、ヒト人工多能性幹細胞を培養器に対して１．０×１０４～１．０×１０６ｃｅｌｌｓ／ｃｍ２の播種密度で培地に採種し、二次元培養する工程を有する。脳オルガノイドの製造方法は、前記ヒト人工多能性幹細胞の培養方法によって得られたヒト人工多能性幹細胞の培養物を、ＢＭＰ阻害剤、及び形質転換因子β（ＴＧＦβ）阻害剤を含有する培地で培養し、細胞凝集塊を形成する工程１と、前記細胞凝集塊を、Ｗｎｔシグナル伝達経路増強剤、及び細胞外マトリックスを含有する培地で培養する工程２と、前記工程２で得られた培養物を攪拌培養する工程３と、を有する。
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公开(公告)号：WO2022002918A1
公开(公告)日：2022-01-06
申请号：PCT/EP2021/067816
申请日：2021-06-29
申请人： C-LECTA GMBH
发明人： VOGEL, Andreas , FELLER, Claudia , BAYER, Christopher David , HOFFMANN, Gregor , SCHÖNERT, Stefan
摘要： The invention relates to a process for the preparation of a glycosylated organic compound by in vitro glycosylation of an organic compound having a nucleophilic group with a saccharide under catalysis of a Leloir glycosyltrans-ferase system comprising at least a first glycosyl transferase and a second glycosyl transferase. The invention further relates to a composition comprising a glycosylated organic compound that is obtainable by the process according to the invention.
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9.发明申请
公开(公告)号：WO2020163935A1
公开(公告)日：2020-08-20
申请号：PCT/BR2020/050041
申请日：2020-02-14
申请人： BRASKEM S.A.
发明人： KOCH, Daniel Johannes , GALZERANI, Felipe , RADUAN ALEXANDRINO, Paulo Moises , MORIN, Nicolas , FRANÇOI, Jean Marie
IPC分类号： C12P13/04 , C12P7/44 , C12N9/04 , C12N9/88 , C12N9/00 , C12P7/42 , C12N15/63 , C12P7/40 , C12P19/32
摘要： The present invention provides biochemical pathways, glyoxylate producing recombinant microorganisms, and methods for the production and yield improvement of glycolic acid and/or glycine via a reverse glyoxylate shunt. The reverse glyoxylate shunt comprises an enzyme that catalyzes the carboxylation of phosphoenol pyruvate (PEP) to oxaloacetate (OAA), or an enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate (OAA) or an enzyme that catalyzes the carboxylation of pyruvate to malate or a combination of any of the previous reactions; an enzyme that catalyzes the conversion of malate to malyl-CoA; an enzyme that catalyzes the conversion of malyl-CoA to glyoxylate and acetyl-CoA; and optionally an enzyme that catalyzes the conversion of oxaloacetate (OAA) to malate. Glyoxylate is reduced to produce glycolate. Alternatively, glyoxylate is converted to glycine. The reverse glyoxylate shunt pathway of the present invention can be utilized synergistically with other glycolic acid and/or glycine producing pathways to increase product yield.
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公开(公告)号：WO2020073026A1
公开(公告)日：2020-04-09
申请号：PCT/US2019/054897
申请日：2019-10-04
发明人： ZHANG, Yong , DAI, Zhefu , ZHANG, Xiao-Nan
IPC分类号： C07H19/207 , C07H21/02 , C12N9/12 , C12P19/32 , C12P19/36
摘要： This disclosure provides a method of synthesis of 4'-thioribose NAD+ and analogues thereof, using an efficient chemoenzymatic approach. Also provided are methods of inhibiting the CD38 enzyme and compounds including 4'-thioribose NAD+ and compounds related thereto.
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