公开(公告)号：EP1404848A2

公开(公告)日：2004-04-07

申请号：EP02732689.1

申请日：2002-04-30

申请人： Icon Genetics AG

发明人： KOOP, Hans-Ulrich ,  MÜHLBAUER, Stefan ,  KLAUS, Sebastian ,  EIBL, Christian ,  HUANG, Fong-Chin ,  GOLDS, Timothy, J.

IPC分类号： C12N15/82

CPC分类号： C12N15/8212 ,  C12N15/821 ,  C12N15/8214 ,  C12N15/8257

摘要： A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.

公开(公告)号：EP0626014B1

公开(公告)日：2001-10-24

申请号：EP93905378.1

申请日：1993-02-09

申请人： VERNEUIL RECHERCHE

发明人： LEJEUNE, Fabienne ,  TOURTE, Monique ,  OULMOUDEN, Ahmad ,  KARST, Francis

IPC分类号： C12N15/82 ,  C12N15/54 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N9/1085 ,  A01H5/10 ,  C12N9/10 ,  C12N9/12 ,  C12N15/821 ,  C12N15/8243 ,  C12N15/8261 ,  Y02A40/146

摘要： Plant carrying in its genome one or more genes coding for one or more enzymes of the early stages of the phytosterol biosynthesis pathway. The genes are capable of coding for a mevalonate kinase, a farnesyl disphosphate synthetase or a mevalonyl 5-phosphate kinase and can be the genes ERG12, ERG20 and ERG8. The plants in which these genes are introduced in a stable manner have a number of advantages including increased development, enhanced productivity and regenerative ability.

公开(公告)号：EP3368676A1

公开(公告)日：2018-09-05

申请号：EP16763144.9

申请日：2016-08-26

申请人： Pioneer Hi-Bred International, Inc.

发明人： ANAND, Ajith ,  ARLING, Maren L. ,  DA SILVA CONCEICAO,Alexandre ,  GORDON-KAMM, William James ,  HASTINGS, Craig ,  HOERSTER, George J. ,  KLEIN, Theodore M. ,  LA ROTA, Carlos M. ,  LOWE, Keith S. ,  TIWARI, Shiv Bahadur ,  WANG, Ning ,  WU, Xinli Emily

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8213 ,  A01H4/008 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/821 ,  C12N15/8216

摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

公开(公告)号：EP3269818A1

公开(公告)日：2018-01-17

申请号：EP17188292.1

申请日：2007-08-31

申请人： Monsanto Technology LLC

发明人： ROUT, Jyoti R.

IPC分类号： C12N15/82

CPC分类号： C12N15/8205 ,  C12N15/8201 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8265

摘要： The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.

公开(公告)号：EP2054517B1

公开(公告)日：2017-10-04

申请号：EP07841704.5

申请日：2007-08-31

申请人： Monsanto Technology, LLC

发明人： ROUT, Jyoti, R.

IPC分类号： C12N15/82

CPC分类号： C12N15/8205 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8206 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q1/6827 ,  C12Q1/686

摘要： The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.

摘要翻译： 本发明提供了鉴定再生的转化植物和分化的转化的植物部分的方法，所述方法在再生细胞以获得分化的组织之前不经过选择性条件而获得。 在具体的实施方案中，植物细胞是玉米植物细胞。 还提供了种植和处理植物的方法，包括鉴定具有特定感兴趣特征的植物。

公开(公告)号：EP3180436A2

公开(公告)日：2017-06-21

申请号：EP16778901.5

申请日：2016-08-24

申请人： Honda Motor Co., Ltd.

发明人： YAMAGUCHI, Asuka ,  OKUMA, Jiro ,  KONDO, Yasuhiro ,  HIROSE, Yoshitsugu

IPC分类号： C12N15/82

CPC分类号： C12N15/8209 ,  C12N15/821 ,  C12N15/8214

摘要： A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.

摘要翻译： （a）中，将编码具有中和亚硝酸毒性功能的蛋白质的基因导入植物组织的方法（a），以及在该方法中插入了基因的植物组织的方法（b），制备质体转化体的方法 （a）在含有野生型植物细胞生长被抑制的浓度的亚硝酸盐氮的培养基或抑制野生型植物再生的浓度的培养基中培养，从而筛选出通过将该基因插入到质体基因组中获得的质体转化体 。

公开(公告)号：EP2971053A4

公开(公告)日：2016-09-28

申请号：EP14765017

申请日：2014-03-14

申请人： DOW AGROSCIENCES LLC

发明人： SAMUEL JAYAKUMAR P ,  CHENNAREDDY SIVARAMA R ,  BOWLING ANDY ,  WHITTECK JOHN ,  MINNICKS TATYANA ,  PAREDDY DAYAKAR ,  YAU KERRM Y ,  SMITH KELLEY A

IPC分类号： C12Q1/00 ,  C12N15/00 ,  C12N15/11

CPC分类号： C12N15/821

摘要： The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants which are comprised of a combination of soybean non-germline transformants and soybean germline transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are detected and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the transformation process.

摘要翻译： 本公开部分地涉及一种方法，用于从大豆转化的群体哪个是由大豆非种系的转化和大豆种系的转化的组合的识别大豆的种系转化。 大豆非种系鉴定转化和在转化过程中早期除去。 被检测并且选择用于培养成成熟的大豆植物大豆的种系转化。 该方法适用于筛选和在转变过程随手获取大豆种系转化体在早期阶段。

公开(公告)号：EP2300617B1

公开(公告)日：2016-03-23

申请号：EP09790552.5

申请日：2009-07-16

申请人： Monsanto Technology LLC

发明人： GILBERTSON, Larry ,  JOHNSON, Susan, Jane

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8218 ,  C12N15/8241

摘要： Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.

公开(公告)号：EP2975129A1

公开(公告)日：2016-01-20

申请号：EP15171145.4

申请日：2006-05-26

申请人： Monsanto Technology LLC

发明人： MALVEN, Marianne ,  RINEHART, Jennifer ,  TAYLOR, Nancy ,  DICKINSON, Ellen

IPC分类号： C12N15/82 ,  C12Q1/68 ,  A01H5/10

CPC分类号： C12N15/8275 ,  A01H1/02 ,  A01H1/04 ,  A01H5/10 ,  A01N57/20 ,  C12N15/821 ,  C12N15/8286 ,  Y02A40/162 ,  A01N2300/00

摘要： The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.

摘要翻译： 本发明提供了包含转化事件MON89788的大豆植物和种子以及这些事件独有的DNA分子。 本发明还提供了用于检测样品中这些DNA分子的存在的方法。

公开(公告)号：EP2787004A1

公开(公告)日：2014-10-08

申请号：EP13162130.2

申请日：2013-04-03

申请人： Malaysian Palm Oil Board

发明人： Ong Abdullah, Meilina ,  Ooi, Siew Eng ,  Ishak, Feshah ,  Smulders, Marinus Johannes Maria ,  Esselink, Gerhard ,  Van der Linden, Cornelis Gerardus

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C07K14/415 ,  C12N15/821 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/154

摘要： The present invention provides a method for predicting a mantled phenotype in oil palm plants, comprising analyzing DNA material from tissue samples oil palm plants for the degree of methylation in a genomic region contained in the genome of the oil palm plant. The method of the invention can be applied for predicting a mantled phenotype in oil palm plants, in particular for predicting in young oil palms the development of a mantled phenotype in a later stage of their life cycle. Method of the invention can also be applied to identify cultures with a high risk of producing ramets that will develop a mantled phenotype. The invention thus also relates to a method to minimize the percentage of oil palm plants with a mantled phenotype in a culture. The invention also provides an isolated polynucleotide for use as a biomarker in a method to predict a mantled phenotype in oil palms.

摘要翻译： 本发明提供用于油棕榈植物预测一个mantled表型，包括从组织样品油棕榈植物分析DNA材料的甲基化中所含的油棕榈计划基因组的基因组区域的程度的方法。 本发明的方法可应用于预测油棕榈植物mantled表型，特别是在年轻的油棕预测一个mantled表型的发展在其生命周期的后期阶段。 因此，本发明的方法可用于识别与生产thatwill开发mantled表型分株的高风险文化。 因此，本发明如此涉及一种方法，以最小化油棕榈植物与在培养mantled的表型的百分比。 因此本发明提供在用作以预测油棕mantled的表型的方法的生物标记物分离的多核苷酸。