公开(公告)号：EP2120546A2

公开(公告)日：2009-11-25

申请号：EP07849571.0

申请日：2007-12-13

申请人： Ben Gurion University Of The Negev

发明人： COHEN, Smadar ,  DVIR-GINZBERG, Mona

IPC分类号： A01N1/00

CPC分类号： C12N5/0671 ,  A61L27/20 ,  A61L27/3813 ,  A61L27/3839 ,  A61L27/56 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2533/74

摘要： The present invention relates to bioresorbable scaffolding for tissue regeneration or repair. The present invention describes an epithelial organoid comprising an aggregate of epithelial cells predominantly expressing markers associated with differentiated cell types, and an aggregate which assumes a structure or performs a function associated with an epithelial organ or a fragment thereof. The present invention also provides a method of treating a subject in need of repair or replacement of an organ or a portion thereof; and/or a method of treating a subject with a disease or disorder which impairs or abrogates a liver, kidney, pancreas, thyroid or pituitary function. The present invention also describes an epithelial graft or artificial organ comprising spheroids, organoids or a combination thereof, and a kit for implantable epithelial graft formation comprising organoids.

公开(公告)号：EP2027257A1

公开(公告)日：2009-02-25

申请号：EP07748692.6

申请日：2007-05-24

申请人： Agency for Science, Technology and Research

发明人： DU, Yanan c/o Institute of Bioengineering and Nanotechnology ,  HAN, Rongbin c/o Institute of Bioengineering and Nanotechnology ,  YU, Hanry c/o Institute of Bioengineering and Nanotechnology

IPC分类号： C12N5/06 ,  C12M3/00 ,  C12N5/08

CPC分类号： C12N5/0677 ,  C12N5/0068 ,  C12N5/0671 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54

摘要： The invention provides a surface, wherein said surface comprises (i) a polymer substrate and (ii) sugar groups and peptide groups coupled to said substrate suitable for culturing hepatocytes.

公开(公告)号：EP2018418A1

公开(公告)日：2009-01-28

申请号：EP07732504.1

申请日：2007-04-24

申请人： REINNERVATE LIMITED

发明人： PRZYBORSKI, Stefan, Alexander ,  CAMERON, Neil

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12M25/14 ,  C12N5/0671 ,  C12N2533/30

摘要： We describe a cell culture substrate comprising a polymerised high internal phase emulsion polymer adapted and modified for use in the routine culture of cells in three dimensions; typically mammalian cells and the use of the substrate in a cell culture system for investigation and analysis of proliferation, differentiation and function of cells.

公开(公告)号：EP1806394A4

公开(公告)日：2008-10-22

申请号：EP05795896

申请日：2005-10-21

申请人： KITAKYUSHU FOUNDATION

发明人： NAKAZAWA KOHJI ,  FUKUDA JUNJI

IPC分类号： C12M3/00 ,  C12N11/02

CPC分类号： C12N5/0671 ,  B33Y80/00 ,  C12M23/16 ,  C12M25/06

摘要： A microchip that is capable of not only forming cellular tissues of uniform configuration and size but also culturing thus formed cellular tissues over a prolonged period of time. There is provided a cellular tissue microchip comprising multiple cell retaining cavities (12) for cell retention, the cell retaining cavities each having bottom plane (20) including one adherent region (30) that exhibits adherence to cells and non-adherent region (32) that surrounds the adherent region (30) and exhibits non-adherence to cells.

公开(公告)号：EP1961809A1

公开(公告)日：2008-08-27

申请号：EP08003023.2

申请日：2008-02-19

申请人： FUJIFILM Corporation

发明人： Iwanaga, Hiroshi ,  Shiratsuchi, Kentaro ,  Nakazawa, Koji ,  Shimomura, Masatsugu

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12N5/0062 ,  C12N5/0671 ,  C12N5/0677 ,  C12N2533/30

摘要： The present invention provides a tissue construct-forming substrate for forming a three-dimensional tissue construct containing proliferating cells, the substrate including a porous film having through-holes, and the porous film having, on the surface of the film, a cell adhesive region capable of retaining cells and a cell non-adhesive region located at a peripheral region of the cell adhesive region, a tissue construct-forming kit comprising the above-mentioned tissue construct-forming substrate and a frame, and a method for forming the above-mentioned tissue construct.

摘要翻译： 本发明提供用于形成三维组织构建含有增殖细胞的组织构建物形成的基材，包括基材的多孔膜具有通孔，并且所述多孔膜的表面上形成具有薄膜中，细胞粘合区 能够保持细胞和位于小区粘合剂区域的周边区域中的细胞非粘接区域，组织构建体形成试剂盒，包括上述的组织构建形成基材和框架，和用于形成上文的方法的 提到组织构建。

公开(公告)号：EP1326968B1

公开(公告)日：2007-04-18

申请号：EP01981506.7

申请日：2001-10-12

申请人： Agency for Science, Technology and Research ,  The National University of Singapore

发明人： YU, Harry ,  LEONG, Kam, W. ,  CHIA, Ser-Mien

IPC分类号： C12N11/04 ,  C12N11/06 ,  C12N11/16

CPC分类号： C12N5/0062 ,  A61K2035/128 ,  C12N5/0012 ,  C12N5/0671 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/72 ,  Y10S514/963 ,  Y10S514/97

摘要： A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.

公开(公告)号：EP1187909B1

公开(公告)日：2005-02-02

申请号：EP00930192.0

申请日：2000-04-28

申请人： MASSACHUSETTS GENERAL HOSPITAL

发明人： VACANTI, Joseph, P. ,  BORENSTEIN, Jeffrey T. ,  PIEN, Homer, Dr. ,  CUNNUNGHAM, Brian T.

IPC分类号： C12N5/00 ,  A61L27/38

CPC分类号： A61L27/3604 ,  A61K35/12 ,  A61K35/44 ,  A61L27/38 ,  B33Y70/00 ,  C12N5/0671 ,  C12N5/0697 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2535/10

摘要： A method and materials to create complex vascularized living tissue in three dimensions from a two-dimension microfabricated mold has been developed. The method involved creating a two dimensional surface having a branching structure etched into the surface. The pattern begins with one or more large channels which serially branch into a large array of channels as small as individual capillaries, then converge to one or more large channels. The etched surface serves a template within a mold formed with the etched surface for the circulation of an individual tissue or organ. Living vascular cells are then seeded onto the mold, where they form living vascular channels based on the pattern etched in the mold. Once formed and sustained by their own matrix, the top of the mold is removed. The organ or tissue specific cells are then added to the etched surface, where they attach and proliferate to form a thin, vascularized sheet of tissue. The tissue can then be gently lifted from the mold using techniques such as fluid flow and other supporting material, as necessary. The tissue can then be systematically folded and compacted into a three-dimensional vascularized structure. This structure can then be implanted into animals or paitents by directly connecting the blood vessels to flow into and out of the device. Immediate perfusion of oxygenated blood occurs, which allows survival and function of the entire living mass.

摘要翻译： 的方法和材料，以在从二维微制造模具三维创建复杂的血管化活组织已经研制成功。 该方法涉及产生具有蚀刻到表面上的分支结构的二维表面。 该图案与一个或多个大的通道哪个串联分支成一个大的阵列的通道小到单个毛细管的开始，然后收敛到一个或多个大的通道。 蚀刻表面供应以用于个体的组织或器官的循环蚀刻表面形成的模具中的模板。 然后活血管细胞接种到模具中，在此它们形成基于蚀刻到模具上的图案住血管通道。 一旦形成并以自己的矩阵持续，模具的顶部被去除。 然后将器官或组织特异性的细胞加入到蚀刻的表面，在那里它们附着和增殖，以形成组织的薄的血管化片材。 组织然后可以轻轻地从使用的技术的模具抬起：如流体流动和其他辅助材料，如必要的。 组织然后可以系统地折叠并压制成三维血管结构。 该结构可以随后通过直接连接血管流入和流出所述装置的被植入到动物或paitents。 含氧血的灌注立即发生，这使得整个生活质量的生存和功能。

公开(公告)号：EP1428871A1

公开(公告)日：2004-06-16

申请号：EP02755656.2

申请日：2002-07-25

申请人： Kataoka, Kazunori

发明人： KATAOKA, Kazunori ,  OTSUKA, Hidenori, c/o Biomaterials Center ,  OKANO, Teruo ,  NAGASAKI, Yukio ,  HORIIKE, Yasuhiro

IPC分类号： C12N5/06

CPC分类号： C12N5/0671 ,  C12N5/0697 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2535/10

摘要： Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.

摘要翻译： 实质细胞中培养在其上已经由一个特定的亲水性聚合物的表面分离，并且其已被图案化的耕地内皮细胞或栽培成纤维细胞。 其含有由此形成的培养的实质细胞的球状体图案化的培养从而由本发明提供。 这种文化维护功能所有这一切是特定的实质细胞在一段较长的时间。

公开(公告)号：EP1339832A1

公开(公告)日：2003-09-03

申请号：EP01999638.8

申请日：2001-12-07

申请人： UNIVERSITY OF THE WEST OF ENGLAND, BRISTOL

发明人： PURCELL, WendyFaculty of Applied Sciences ,  XU, JinshengFaculty of Applied Sciences

IPC分类号： C12N5/06 ,  C12N5/08 ,  C12M1/02 ,  G01N33/50

CPC分类号： C12N5/0671 ,  A01N1/0205 ,  A01N1/0278 ,  C12N2503/00 ,  C12N2503/04

摘要： There is disclosed a method for the formation of spheroids comprising: (a) obtaining and/or deriving a plurality of cells from a tissue or an organ or from a part thereof; (b) orbitaly rotating the cells in a fluid medium at a first speed and for a time sufficient for spheroids to form; and (c) further orbitally rotating the spheroids formed in step (b) in a fluid medium at a second speed or speeds, slower than said first speed.

公开(公告)号：EP1181355A2

公开(公告)日：2002-02-27

申请号：EP98909974.2

申请日：1998-01-09

申请人： YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM

发明人： MITRANI, Eduardo, N.

IPC分类号： C12N5/06

CPC分类号： C12M25/14 ,  A61M1/3472 ,  A61M1/3489 ,  C12M29/10 ,  C12N5/0671

摘要： A device for performing a biological modification of a fluid, particularly in order to assist or replace the functioning of an organ which normally performs this modification, including a collection of liver micro-organ cultures. The device of the present invention is preferably directly connected to a subject for performing this modification.