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230 条记录 (耗时 0.038 秒)

    METHOD FOR INCORPORATING ALIPHATIC AMINO ACIDS COMPRISING ALKYNE, AZIDE OR ALIPHATIC KETONE FUNCTIONAL GROUPS USING APPROPRIATE TRNA/TRNA SYNTHASE PAIRS
    11.
    发明公开
    METHOD FOR INCORPORATING ALIPHATIC AMINO ACIDS COMPRISING ALKYNE, AZIDE OR ALIPHATIC KETONE FUNCTIONAL GROUPS USING APPROPRIATE TRNA/TRNA SYNTHASE PAIRS 有权
    METHOD FOR与具有相关的tRNA酶/ tRNA-SYNTHASEPAAREN脂族氨基炔叠氮化或脂族酮FUNCTIONAL RECORDING

    公开(公告)号：EP2438174A2

    公开(公告)日：2012-04-11

    申请号：EP10727116.5

    申请日：2010-06-02

    申请人： Medical Research Council Technology ,  North Carolina State University

    发明人： NGUYEN, Duy, P. ,  NEUMANN, Heinz ,  DEITERS, Alexander ,  CHIN, Jason ,  LUSIC, Hrvoje

    IPC分类号： C12N15/67

    CPC分类号： C12N15/67 ,  C07C233/49 ,  C07C271/22 ,  C12N9/93 ,  C12P21/02

    摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

    摘要翻译： 本发明涉及一种制造多肽，其包含正交官能团的方法，所述正交官能团被在脂肪族氨基酸或氨基酸衍生物组成，所述方法包括提供宿主细胞; 提供核酸编码感兴趣的多肽; 提供的tRNA-tRNA合成酶对正交于所述宿主细胞; 添加于氨基酸或氨基酸衍生物包含所关注的正交官能团，worin所述氨基酸或氨基酸衍生物为所述正交tRNA合成酶底物一，worin所述氨基酸或氨基酸衍生物具有脂族碳主链; 孵育，并允许所说的氨基酸或氨基酸衍生物的掺入的经由正交tRNA合成酶-tRNA对感兴趣的多肽。 因此，本发明涉及某些氨基酸，以及多肽，其包含相同。

    METHOD FOR ANALYSIS/IDENTIFICATION OF ANTIBODY GENE AT ONE-CELL LEVEL
    13.
    发明公开
    METHOD FOR ANALYSIS/IDENTIFICATION OF ANTIBODY GENE AT ONE-CELL LEVEL 有权
    VERFAHREN ZUR ANALYZE / IDENTIFIZIERUNG EINESANTIKÖRPERGENSAUF EINZELZELLEBENE

    公开(公告)号：EP2316936A1

    公开(公告)日：2011-05-04

    申请号：EP09758131.8

    申请日：2009-06-04

    申请人： Shizuoka Prefecture

    发明人： AKIYAMA, Yasuto

    IPC分类号： C12N15/09 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68

    CPC分类号： G01N33/56972 ,  G01N33/6854

    摘要： Disclosed are: a method for identifying/analyzing a gene for an antibody in one cell derived from a human; a technique for producing an antibody derived from an identified one B cell; and others. A gene for an antibody specific to a melanoma antigen is analyzed/identified at a one-cell level by using an immortalized B cell produced from peripheral blood monocytes from a melanoma patient or a primary B cell included in the peripheral blood monocytes. It is found that B cells capable of producing a specific antibody can be separated on one cell by one cell basis by staining the B cells with a GST-labeled melanoma-specific cancer antigen MAGE1, an Alexa-labeled anti-GST antibody and a PE-labeled anti-human IgG antibody and carrying out the single cell sorting of the stained B cells. Further, a practical technique for extracting total RNA from the separated one B cell and cloning a gene for a specific antibody into the total RNA efficiently can be established.

    摘要翻译： 公开了一种用于鉴定/分析来源于人的一种细胞中的抗体基因的方法; 用于产生源自鉴定的一种B细胞的抗体的技术; 和别的。 通过使用由来自黑素瘤患者的外周血单核细胞或包含在外周血单核细胞中的原代B细胞产生的永生化B细胞，在单细胞水平分析/鉴定用于黑素瘤抗原特异性抗体的基因。 发现能够产生特异性抗体的B细胞可以通过用GST标记的黑素瘤特异性癌抗原MAGE1，Alexa标记的抗GST抗体和PE染色B细胞，在一个细胞上通过一个细胞分离 标记的抗人IgG抗体，并进行染色的B细胞的单细胞分选。 此外，可以建立从分离的一个B细胞中提取总RNA并将特异性抗体的基因有效克隆到总RNA中的实用技术。

    AGENT FOR TREATING SKIN AGING AND SCARS
    16.
    发明公开
    AGENT FOR TREATING SKIN AGING AND SCARS 有权
    MITTEL ZUR BEHANDLUNG VON HAUTALTERUNG UND NARBEN

    公开(公告)号：EP2263683A1

    公开(公告)日：2010-12-22

    申请号：EP09723909.9

    申请日：2009-03-24

    申请人： Labo Juversa Co., Ltd.

    发明人： ONO, Ichiro

    IPC分类号： A61K38/22 ,  A61K8/64 ,  A61P17/00 ,  A61P17/02 ,  A61P17/16 ,  A61Q19/08

    CPC分类号： A61K9/0019 ,  A61K8/66 ,  A61K9/0021 ,  A61K38/1825 ,  A61K2800/91 ,  A61Q19/08

    摘要： It is to provide an agent for preventing and treating skin aging, or an agent for treating skin scar that can exert a sufficient effect. An agent for preventing and treating skin aging comprising bFGF for treating aging of the skin that is administered intradermally or subcutaneously, or an agent for treating skin scar comprising bFGF for treating scar of skin that is administered intradermally or subcutaneously is utilized. Preferred examples of aging of skin include skin wrinkle, pigmented spot, sagging skin, rough skin, skin thinning, decrease of skin viscoelasticity, etc., and preferred examples of scar include keloid, hypertrophic scar, scar contracture, etc.

    摘要翻译： 它是提供一种用于预防和治疗皮肤衰老的药剂，或用于治疗可发挥足够效果的皮肤瘢痕的药剂。 利用包含用于治疗皮内或皮下给药的皮肤老化的bFGF或用于治疗皮肤瘢痕的皮肤瘢痕的试剂，用于预防和治疗皮肤老化的试剂，其包含用于治疗皮内或皮下给药的皮肤瘢痕的bFGF。 皮肤老化的优选实例包括皮肤皱纹，着色点，下垂皮肤，粗糙皮肤，皮肤变薄，皮肤粘弹性降低等，并且瘢痕疙瘩，肥厚性瘢痕，瘢痕挛缩等的优选实例。

    DIAGNOSTIC METHODS FOR HIV INFECTION
    17.
    发明公开
    DIAGNOSTIC METHODS FOR HIV INFECTION 有权
    诊断程序艾滋病毒感染

    公开(公告)号：EP2210112A1

    公开(公告)日：2010-07-28

    申请号：EP08806601.4

    申请日：2008-10-15

    申请人： King's College London ,  Baylor College Of Medicine

    发明人： VYAKARNAM, Annapurna ,  ROWLEY, David, R.

    IPC分类号： G01N33/68 ,  G01N33/569

    CPC分类号： C07K16/38 ,  A61K31/7088 ,  A61K2039/505 ,  C07K14/47 ,  C07K14/705 ,  C07K2317/76 ,  G01N33/56988

    摘要： The invention relates to a method of aiding the diagnosis of a human immunodeficiency virus infection in a subject, said method comprising (i) providing a sample from the subject (ii) determining the level of ps20 in said sample (iii) comparing the level of ps20 of (ii) with the level of ps20 in an uninfected reference sample, wherein a higher level of ps20 in the sample from the subject compared to the uninfected reference sample indicates an increased likelihood of human immunodeficiency virus infection in said subject. The invention also relates to methods for assessing susceptibility of a subject to human immunodeficiency virus infection. Most suitably the ps20 level is determined via binding by an anti-ps20 antibody such as the IG7 antibody. The invention also relates to kits for use in said methods.

    DETERMINATION METHOD FOR ALLERGIC DISEASE
    19.
    发明公开
    DETERMINATION METHOD FOR ALLERGIC DISEASE 有权
    BESTIMMUNGSVERFAHRENFÜREINE ALLERGISCHE ERKRANKUNG

    公开(公告)号：EP2136211A1

    公开(公告)日：2009-12-23

    申请号：EP08710396.6

    申请日：2008-02-15

    申请人： The University of Tokushima ,  Oriental Yeast Co., Ltd.

    发明人： KIDO, Hiroshi ,  SUZUKI, Koichi

    IPC分类号： G01N33/53 ,  G01N21/78 ,  G01N33/531 ,  G01N33/543

    CPC分类号： G01N33/6854 ,  G01N33/54373

    摘要： It is to provide a method for determining allergic diseases with a high sensitivity and accuracy which enables multilateral and global analysis with a minute amount of sample, even using body fluid other than blood such as saliva, nasal discharge and tears as a sample, particularly by suppressing nonspecific reaction as much as possible. A chemically modified diamond/DLC (Diamond-like Carbon) chip is activated with a reacting reagent, a coupling reaction with a peptide comprising allergen or allergen epitope is conducted, and a sample such as saliva, tears, and nasal discharge which has undergone pressure filtration with a low protein-adsorbing filter, is contacted with an allergen determination chip to which washing and blocking operations have been performed on unreacted active groups, and an allergen recognizing antibody in the sample captured by the allergen determination chip is detected by immunoassay using a labeled secondary antibody, wherein a glycine-containing solution is used for a washing solution and/or blocking solution used in the washing and blocking operations, is used.

    摘要翻译： 它是提供一种以高灵敏度和精确度确定过敏性疾病的方法，其能够使用少量样品进行多边和全球分析，甚至使用血液以外的体液（如唾液，鼻腔排泄物和泪液）作为样品，特别是通过 尽可能地抑制非特异性反应。 用反应试剂激活化学改性的金刚石/ DLC（类金刚石碳）芯片，进行与包含变应原或变应原表位的肽的偶联反应，以及经历压力的样品例如唾液，泪液和鼻放电 与低蛋白质吸附过滤器的过滤与已对未反应的活性基团进行洗涤和封闭操作的变应原测定芯片接触，并且通过免疫测定法检测由变应原测定芯片捕获的样品中的变应原识别抗体，使用 使用其中含有甘氨酸的溶液用于洗涤和阻塞操作中使用的洗涤溶液和/或封闭溶液。