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    Multi-gene expression constructs containing modified inteins
    11.
    发明公开
    Multi-gene expression constructs containing modified inteins 审中-公开
    含有修饰内含子的多基因表达构建体

    公开(公告)号：EP2159287A2

    公开(公告)日：2010-03-03

    申请号：EP09075515.8

    申请日：2001-02-09

    申请人： METABOLIX, INC.

    发明人： Snell, Kristi D.

    IPC分类号： C12N15/82 ,  C12N15/63 ,  C12P7/62

    CPC分类号： C12N15/8216 ,  C12P7/625

    摘要： Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5' promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5'-terminus of the coding regions for the second protein to form a construct for expression of more than two proteins. A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and/or value added products, illustrated by the production of polyhydroxyalkanoates in plants.

    摘要翻译： 描述了使用单一转化事件将多个基因导入植物的方法和构建体。 构建体含有与编码修饰的内含肽剪接单位的DNA可操作连接的单个5'启动子。 拼接单元表达为多蛋白并且由与融合至第二蛋白质的内含肽融合的第一蛋白质组成。 剪接单位已被设计用于促进多蛋白中所有非必需组分的切除，但阻止通常与蛋白质剪接相关的连接反应。 编码内含肽和额外蛋白质的额外遗传元件可以与第二蛋白质的编码区的5'端一致地融合，以形成用于表达两种以上蛋白质的构建体。 当构建体将在真核细胞中表达时，单个3'终止序列，例如聚腺苷酸化序列，遵循最后的编码序列。 这些方法和构建体对于产生具有堆叠的输入性状的植物特别有用，如产生BT毒素的草甘膦耐受植物和/或增值产物所示，通过在植物中生产聚羟基链烷酸酯来说明。

    POLYHYDROXYALKANOATE COMPOSITIONS HAVING CONTROLLED DEGRADATION RATES
    15.
    发明授权
    POLYHYDROXYALKANOATE COMPOSITIONS HAVING CONTROLLED DEGRADATION RATES 有权
    具有可控降解速率的聚羟基烷酸酯组合物

    公开(公告)号：EP1042388B1

    公开(公告)日：2006-11-15

    申请号：EP98964258.2

    申请日：1998-12-22

    申请人： METABOLIX, INC.

    发明人： MARTIN, David, P. ,  SKRALY, Frank, A. ,  WILLIAMS, Simon, F.

    IPC分类号： C08G63/06 ,  C08G63/91 ,  C08K3/00 ,  C08K5/00 ,  C08J9/26 ,  C08J9/28 ,  C08L67/04 ,  C12P7/62 ,  A61K47/34 ,  A61L17/00 ,  A61L27/00 ,  A61L31/00

    CPC分类号： A61L31/06 ,  A61K9/1647 ,  A61K9/204 ,  A61K47/34 ,  A61K47/593 ,  A61L15/26 ,  A61L17/12 ,  A61L24/0042 ,  A61L26/0019 ,  A61L26/009 ,  A61L27/18 ,  A61L27/34 ,  A61L27/58 ,  A61L29/06 ,  A61L31/148 ,  C08G63/06 ,  C08G63/60 ,  C08G63/64 ,  C08G63/664 ,  C08G63/6852 ,  C08G63/6882 ,  C08J9/26 ,  C08J2201/044 ,  C08J2201/0482 ,  C08J2367/04 ,  C08K5/0033 ,  C08L67/04 ,  C08L101/00 ,  C12P7/625 ,  C08L2666/02

    摘要： Biocompatible polyhydroxyalkanoate compositions, particularly based on biotechnologically produced homo- and copolymers of 4- hydroxybutyric acid, with controlled degradation rates have been developed. In one embodiment, the polyhydroxyalkanoates contain additives like, for example, pore forming agents to alter the degradation rates. In another embodiment, the polyhydroxyalkanoates are formed of mixtures of monomers or include pendant groups or modifications in their backbones to alter their degradation rates. In still another embodiment, the polyhydroxyalkanoates are chemically modified. Methods for manufacturing the devices which increase porosity or exposed surface area can be used to alter degradability. As demonstrated by the examples, these polyhydroxyalkanoate compositions have extremely favorable mechanical properties, as well as are biocompatible and degrade within desirable time frames under physioogical conditions. These polyhydroxyalkanoate materials provide a wider range of polyhydroxyalkanoate degradation rates than are currently available. Methods for processing these materials, particularly for therapeutic, prophylactic or diagnostic applications, or into devices which can be implanted or injected, are also described.

    摘要翻译： 已经开发了具有可控降解速率的生物相容性聚羟基链烷酸酯组合物，特别是基于生物技术生产的4-羟基丁酸均聚物和共聚物的组合物。 在一个实施方案中，聚羟基链烷酸酯含有添加剂，例如成孔剂以改变降解速率。 在另一个实施方案中，聚羟基链烷酸酯由单体混合物形成或在其骨架中包含侧基或修饰以改变其降解速率。 在又一个实施方案中，聚羟基链烷酸酯是化学改性的。 制造增加孔隙率或暴露表面积的装置的方法可用于改变可降解性。 如实施例所证实的，这些聚羟基链烷酸酯组合物具有非常有利的机械性能，并且在理疗条件下在所需的时间范围内具有生物相容性和降解性。 这些聚羟基链烷酸酯材料提供比目前可获得的更宽范围的聚羟基链烷酸酯降解速率。 还描述了用于处理这些材料的方法，特别是用于治疗，预防或诊断应用，或者可以植入或注射的装置。

    Polyhydroxyalkanoate compositions having controlled degradation rates
    17.
    发明公开
    Polyhydroxyalkanoate compositions having controlled degradation rates 有权
    具有可控降解速率的聚羟基链烷酸酯组合物

    公开(公告)号：EP1659142A1

    公开(公告)日：2006-05-24

    申请号：EP06075303.5

    申请日：1998-12-22

    申请人： METABOLIX, INC.

    发明人： The designation of the inventor has not yet been filed

    IPC分类号： C08G63/06 ,  C08G63/91 ,  C08K3/00 ,  C08K5/00 ,  C08J9/26 ,  C08J9/28 ,  C08L67/04 ,  C12P7/62 ,  A61K47/34 ,  A61L17/00 ,  A61L27/00 ,  A61L31/00

    CPC分类号： A61L31/06 ,  A61K9/1647 ,  A61K9/204 ,  A61K47/34 ,  A61K47/593 ,  A61L15/26 ,  A61L17/12 ,  A61L24/0042 ,  A61L26/0019 ,  A61L26/009 ,  A61L27/18 ,  A61L27/34 ,  A61L27/58 ,  A61L29/06 ,  A61L31/148 ,  C08G63/06 ,  C08G63/60 ,  C08G63/64 ,  C08G63/664 ,  C08G63/6852 ,  C08G63/6882 ,  C08J9/26 ,  C08J2201/044 ,  C08J2201/0482 ,  C08J2367/04 ,  C08K5/0033 ,  C08L67/04 ,  C08L101/00 ,  C12P7/625 ,  C08L2666/02

    摘要： Biocompatible polyhydroxyalkanoate compositions, particularly based on biotechnologically produced homo- and copolymers of 4- hydroxybutyric acid, with controlled degradation rates have been developed. In one embodiment, the polyhydroxyalkanoates contain additives like, for example, pore forming agents to alter the degradation rates. In another embodiment, the polyhydroxyalkanoates are formed of mixtures of monomers or include pendant groups or modifications in their backbones to alter their degradation rates. In still another embodiment, the polyhydroxyalkanoates are chemically modified. Methods for manufacturing the devices which increase porosity or exposed surface area can be used to alter degradability. As demonstrated by the examples, these polyhydroxyalkanoate compositions have extremely favorable mechanical properties, as well as are biocompatible and degrade within desirable time frames under physioogical conditions. These polyhydroxyalkanoate materials provide a wider range of polyhydroxyalkanoate degradation rates than are currently available. Methods for processing these materials, particularly for therapeutic, prophylactic or diagnostic applications, or into devices which can be implanted or injected, are also described.

    摘要翻译： 已经开发了具有可控降解速率的生物相容性聚羟基链烷酸酯组合物，特别是基于生物技术生产的4-羟基丁酸均聚物和共聚物的组合物。 在一个实施方案中，聚羟基链烷酸酯含有添加剂，例如成孔剂以改变降解速率。 在另一个实施方案中，聚羟基链烷酸酯由单体混合物形成或在其骨架中包含侧基或修饰以改变其降解速率。 在又一个实施方案中，聚羟基链烷酸酯是化学改性的。 制造增加孔隙率或暴露表面积的装置的方法可用于改变可降解性。 如实施例所证实的，这些聚羟基链烷酸酯组合物具有非常有利的机械性能，并且在理疗条件下在所需的时间范围内具有生物相容性和降解性。 这些聚羟基链烷酸酯材料提供比目前可获得的更宽范围的聚羟基链烷酸酯降解速率。 还描述了用于处理这些材料的方法，特别是用于治疗，预防或诊断应用，或者可以植入或注射的装置。

    POLYMER BLENDS CONTAINING POLYHYDROXYALKANOATES AND COMPOSITIONS WITH GOOD RETENTION OF ELONGATION
    18.
    发明授权
    POLYMER BLENDS CONTAINING POLYHYDROXYALKANOATES AND COMPOSITIONS WITH GOOD RETENTION OF ELONGATION 有权
    含聚羟基烷酸酯使用扩展的良好的耐聚合物共混物和组合物

    公开(公告)号：EP1027384B1

    公开(公告)日：2005-07-27

    申请号：EP98954005.9

    申请日：1998-10-27

    申请人： Metabolix, Inc.

    发明人： ASRAR, Jawed ,  PIERRE, Jean, R.

    IPC分类号： C08L67/00

    CPC分类号： C08L67/04 ,  C08L2666/18

    摘要： Biodegradable polymer blend compositions are provided which contain oligomeric esters. The oligoester compounds can compatibilize blends of two or more biodegradable polymers resulting in excellent ductility and reduced tendency to embrittlement of products produced therefrom. In a preferred embodiment of the invention, the blend composition comprises a PHA, polycaprolactone (PCL) and one or more oligomeric esters. In another preferred embodiment of the invention, the blend composition comprises a PHA, polybutylenesuccinate-adipate (PBSUA), and one or more oligomeric esters.

    POLYHYDROXYALKANOATES AND FILM FORMATION THEREFROM
    20.
    发明授权
    POLYHYDROXYALKANOATES AND FILM FORMATION THEREFROM 失效
    聚羟基脂肪酸酯和拍摄

    公开(公告)号：EP0695321B2

    公开(公告)日：2002-11-06

    申请号：EP94904241.0

    申请日：1994-01-12

    申请人： METABOLIX, INC.

    发明人： WADDINGTON, Simon Dominic

    IPC分类号： C08J5/18 ,  C08L67/04 ,  B32B27/06

    CPC分类号： C08J5/18 ,  C08G63/06 ,  C08J2367/04 ,  Y10T428/1355 ,  Y10T428/2913 ,  Y10T428/31786

    摘要： A supported or cast film of a polyhydroxyalkanoate polymer, such as a polyhydroxybutyrate/valerate polymer, can be prepared by applying a layer of molten polymer to a cool (typically 4 to 20 °C) surface not substantially above the glass transition temperature of the polymer, so as to form a solid, glassy film with a high density of nucleation sites; subsequently the temperature of the film so formed is raised, for example to 40 °C or more, towards the optimum temperature for growth of the polymer's spherulites effectively separating the film formation/nucleation step from the crystallisation step (growth of spherulites around the nucleation sites). Smaller crystallites are formed, and the film has improved barrier properties.