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    BIOABSORBABLE POLYMER CONTAINING 2-HYDROXYACID MONOMERS
    4.
    发明授权
    BIOABSORBABLE POLYMER CONTAINING 2-HYDROXYACID MONOMERS 有权
    含有2-羟基单体的生物可吸收聚合物

    公开(公告)号：EP1654373B1

    公开(公告)日：2012-08-15

    申请号：EP03808364.8

    申请日：2003-05-07

    申请人： METABOLIX, INC. ,  Tepha, Inc.

    发明人： MARTIN, David, P. ,  SKRALY, Frank, A.

    IPC分类号： C12P7/62

    CPC分类号： C12P7/625 ,  Y10T428/31507

    摘要： Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer.

    摘要翻译： 已经开发了用于生产含有2-羟基酸单体的PHA共聚物的基因工程生物体及其制备和使用方法。 含有2-羟基酸单体的共聚物可以通过在活细胞中通过PHA聚合酶的作用进行生物合成来合成。 通过改变细胞的遗传背景，可以控制特定的代谢途径，从而控制PHA聚合物中乙醇酸共聚单体的水平。

    TOUGHENED POLYHYDROXYALKANOATE COMPOSITIONS
    5.
    发明公开
    TOUGHENED POLYHYDROXYALKANOATE COMPOSITIONS 审中-公开
    增韧聚羟基烷酸酯组合物

    公开(公告)号：EP2470605A2

    公开(公告)日：2012-07-04

    申请号：EP10815895.7

    申请日：2010-08-27

    申请人： METABOLIX, INC.

    发明人： WHITEHOUSE, Robert, S.

    IPC分类号： C08L67/04 ,  C08L31/04 ,  C08L29/04 ,  C08G63/06 ,  C08F18/08

    摘要： Toughened polyhydroxyalkanoate resin compositions are disclosed, comprising thermoplastic polyhydroxyalkanoate compositions and a toughener comprising a vinyl acetate homopolymer or copolymer and optionally polyvinyl alcohol.

    摘要翻译： 公开了增韧的聚羟基链烷酸酯树脂组合物，其包含热塑性聚羟基链烷酸酯组合物和包含乙酸乙烯酯均聚物或共聚物和任选的聚乙烯醇的增韧剂。

    Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways
    6.
    发明公开
    Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways 有权
    通过辅酶α依赖性醛脱氢酶途径生产聚羟基链烷酸酯

    公开(公告)号：EP2365088A3

    公开(公告)日：2012-01-11

    申请号：EP10075400.1

    申请日：2003-09-12

    申请人： METABOLIX, INC.

    发明人： Skraly, Frank A.

    IPC分类号： C12P7/62

    CPC分类号： C12N9/0008 ,  C12N15/52 ,  C12P7/625

    摘要： The present invention provides a method of producing polyhydroxyalkanoate (PHA) polymers comprising at least one hydroxyacyl monomer selected from the group consisting of 3-hydroxypropionate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate, 5-hydroxyvalerate, and 3-hydroxyhexanoate, comprising: expressing in an organism genes encoding a polyhydroxyalkanoate (PHA) synthase and a CoA-dependent aldehyde dehydrogenase, feeding a chemical that is converted by the organism to at least one hydroxyacyl-CoA monomer by an enzyme-catalysed pathway that includes the CoA-dependent aldehyde dehydrogenase activity and allowing polymerisation of the at least one hydroxyacyl-CoA monomer by the PHA synthase to form PHA polymers, and recovering the thus-produced PHA polymers, wherein the organism is produced from a wild-type organism having an endogenous CoA-dependent aldehyde dehydrogenase gene which is modified by gene mutation to increase CoA-dependent aldehyde dehydrogenase activity, or wherein the organism is a recombinant organism in which a gene is recombinantly expressed to encode the CoA-dependent aldehyde dehydrogenase. The present invention also provides a recombinant organism for use in the foregoing method. The present invention also provides a method comprising the step of amplifying a DNA sequence encoding the eutE gene from an E. coli genome using the following oligonucleotides primers: 5' GGT GGT ACC TTA AGA GGA GGT TTT TAT GAA TCA ACA GGA TAT TGA ACA-3' (SEQ ID NO: 1) and 5'-GGT GCG GCC GCT TAA ACA ATG CGA AAC GCA TCG-3' (SEQ ID NO: 2).

    摘要翻译： 本发明提供了一种生产包含至少一种选自3-羟基丙酸酯，3-羟基戊酸酯，4-羟基丁酸酯，4-羟基戊酸酯，5-羟基戊酸酯和3-羟基己酸酯的羟基酰基单体的聚羟基链烷酸酯（PHA）聚合物的方法， 包括：在生物体中表达编码聚羟基链烷酸酯（PHA）合酶和CoA依赖性醛脱氢酶的基因，通过酶催化途径将由所述生物体转化的化学物质供给至少一个羟酰基-CoA单体，所述酶催化途径包括所述CoA- 依赖的醛脱氢酶活性并允许所述至少一种羟基酰基-CoA单体通过所述PHA合酶聚合形成PHA聚合物，并且回收由此产生的PHA聚合物，其中所述生物由具有内源性CoA- 通过基因突变修饰以增加辅酶A依赖性醛脱氢酶活性或其中 在生物体中是其中基因被重组表达以编码CoA依赖性醛脱氢酶的重组生物体。 本发明还提供了用于上述方法的重组生物体。 本发明还提供了一种方法，包括使用以下寡核苷酸引物扩增编码来自大肠杆菌基因组的eutE基因的DNA序列的步骤：5'GGT GGT ACC TTA AGA GGA GGT TTT TAT GAA TCA ACA GGA TAT TGA ACA- 3'（SEQ ID NO：1）和5'-GGT GCG GCC GCT TAA ACA ATG CGA AAC GCA TCG-3'（SEQ ID NO：2）。

    Method for making poly(3-hydroxyproprionate)
    7.
    发明公开
    Method for making poly(3-hydroxyproprionate) 有权
    一种用于制备聚方法（3-Hydroxyproprionat）

    公开(公告)号：EP2267133A3

    公开(公告)日：2011-09-14

    申请号：EP10009227.9

    申请日：1999-05-21

    申请人： METABOLIX, INC.

    发明人： Skraly, Frank A. ,  Peoples, Oliver P

    IPC分类号： C12N15/52 ,  C12N15/54 ,  C12N15/70 ,  C12N1/21 ,  C08G63/06 ,  C12P7/62

    CPC分类号： C12N9/93 ,  C08G63/06 ,  C12N9/1029 ,  C12P7/625

    摘要： The present application provides a method for making poly(3-hydroxypropionate) in a biological system comprising providing one or more substrates selected from the group consisting of 3-hydroxypropionate and 1,3-propanediol, wherein the biological system is selected from the group consisting of bacteria, yeast, fungi, and plants; wherein the biological system expresses enzymes selected from the group consisting of polyhydroxyalkanoate synthase, acyl-CoA transferase, hydroxyacyl CoA transferase, and hydroxyacyl CoA synthetase such that poly(3-hydroxypropionate) accumulates. The present application also provides a method for making a polymer comprising 5-hydroxyvalerate in a biological system comprising providing one or more substrates selected from the group consisting of 5-hydroxyvalerate and 1,5-pentanediol, wherein the biological system is selected from the group consisting of bacteria, yeast, fungi, and plants; wherein the biological system expresses enzymes selected from the group consisting of polyhydroxyalkanoate synthase, acyl-CoA transferase, hydroxyacyl CoA transferase, and hydroxyacyl CoA synthetase such that a polymer comprising 5-hydroxyvalerate accumulates.

    Method for making poly(3-hydroxyproprionate)
    9.
    发明公开
    Method for making poly(3-hydroxyproprionate) 有权
    制备聚（3-羟基丙酸酯）的方法

    公开(公告)号：EP2267133A2

    公开(公告)日：2010-12-29

    申请号：EP10009227.9

    申请日：1999-05-21

    申请人： METABOLIX, INC.

    发明人： Skraly, Frank A. ,  Peoples, Oliver P

    IPC分类号： C12N15/52 ,  C12N15/54 ,  C12N15/70 ,  C12N1/21 ,  C08G63/06 ,  C12P7/62

    CPC分类号： C12N9/93 ,  C08G63/06 ,  C12N9/1029 ,  C12P7/625

    摘要： The present application provides a method for making poly(3-hydroxypropionate) in a biological system comprising providing one or more substrates selected from the group consisting of 3-hydroxypropionate and 1,3-propanediol, wherein the biological system is selected from the group consisting of bacteria, yeast, fungi, and plants; wherein the biological system expresses enzymes selected from the group consisting of polyhydroxyalkanoate synthase, acyl-CoA transferase, hydroxyacyl CoA transferase, and hydroxyacyl CoA synthetase such that poly(3-hydroxypropionate) accumulates. The present application also provides a method for making a polymer comprising 5-hydroxyvalerate in a biological system comprising providing one or more substrates selected from the group consisting of 5-hydroxyvalerate and 1,5-pentanediol, wherein the biological system is selected from the group consisting of bacteria, yeast, fungi, and plants; wherein the biological system expresses enzymes selected from the group consisting of polyhydroxyalkanoate synthase, acyl-CoA transferase, hydroxyacyl CoA transferase, and hydroxyacyl CoA synthetase such that a polymer comprising 5-hydroxyvalerate accumulates.

    摘要翻译： 本申请提供了用于在生物系统中制备聚（3-羟基丙酸酯）的方法，其包括提供一种或多种选自3-羟基丙酸酯和1,3-丙二醇的底物，其中所述生物系统选自 细菌，酵母，真菌和植物; 其中所述生物系统表达选自聚羟基链烷酸酯合酶，酰基-CoA转移酶，羟酰CoA转移酶和羟酰CoA合成酶的酶，使得聚（3-羟基丙酸）积累。 本申请还提供了用于在生物系统中制备包含5-羟基戊酸酯的聚合物的方法，其包括提供一种或多种选自5-羟基戊酸酯和1,5-戊二醇的底物，其中生物系统选自 由细菌，酵母，真菌和植物组成; 其中所述生物系统表达选自由聚羟基链烷酸酯合酶，酰基-CoA转移酶，羟基酰基CoA转移酶和羟酰CoA合成酶组成的组的酶，使得包含5-羟基戊酸酯的聚合物积聚。