公开(公告)号：EP2716798B1

公开(公告)日：2019-12-18

申请号：EP12793074.1

申请日：2012-05-30

申请人： Spiber Inc.

发明人： SEKIYAMA Kazuhide ,  SEKIYAMA Kaori ,  ISHIKAWA Mizuki ,  SATO Ryota ,  MURATA Shinya

IPC分类号： C07K14/435 ,  D01D5/16 ,  D01F4/02 ,  D01F6/68 ,  D01D5/06

公开(公告)号：EP3508517A1

公开(公告)日：2019-07-10

申请号：EP17846701.5

申请日：2017-09-01

申请人： Spiber Inc. ,  Tekunohama Co., Ltd.

发明人： HIRAI Shinji ,  UBUKATA Yukimasa ,  ABE Ayumi ,  KOTAKA Koichi ,  NAKAYAMA Yuki ,  NOBA Junichi

IPC分类号： C08J5/00 ,  B29C43/02 ,  C07K14/435 ,  C08H1/00 ,  C08L89/00

摘要： The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.

公开(公告)号：EP3498856A1

公开(公告)日：2019-06-19

申请号：EP17839566.1

申请日：2017-08-10

申请人： Spiber Inc.

发明人： OSAWA Toshiaki ,  SATO Yuya ,  MORITA Keisuke

IPC分类号： C12P21/02 ,  C07K1/14 ,  C07K1/30 ,  C07K1/34 ,  C12P21/00

摘要： An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：EP3351584A1

公开(公告)日：2018-07-25

申请号：EP16846375.0

申请日：2016-09-08

申请人： Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

发明人： SUGAHARA Junichi ,  SEKIYAMA Kaori ,  ABE Ayumi ,  SHIMOKATA Junichi ,  NOBA Junichi ,  HIRAI Shinji

IPC分类号： C08H1/00 ,  B29C43/02

CPC分类号： B29C43/02 ,  B29C43/003 ,  B29K2089/00 ,  C08H1/00

摘要： Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.

公开(公告)号：EP2774934B1

公开(公告)日：2017-11-29

申请号：EP12844908.9

申请日：2012-10-29

申请人： Spiber Inc.

发明人： SUGAHARA Junichi ,  SEKIYAMA, Kaori ,  SATO, Ryota ,  ISHIKAWA, Mizuki ,  MURATA, Shinya ,  SEKIYAMA, Kazuhide ,  OTOMO, Kazuko

IPC分类号： C07K14/435 ,  D01D5/04 ,  D01F4/02 ,  C12N15/09

CPC分类号： C08K5/41 ,  B29D99/0078 ,  C07K14/43518 ,  C08K3/16 ,  C08K5/20 ,  D01D5/04 ,  D01F4/02 ,  D01F6/68 ,  Y10S530/858

摘要： A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(ii): (i) DMSO with an inorganic salt; and (ii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

公开(公告)号：EP2990413A4

公开(公告)日：2016-10-05

申请号：EP14787816

申请日：2014-04-18

申请人： SPIBER INC

发明人： OSAWA TOSHIAKI ,  MORITA KEISUKE

IPC分类号： C07K14/435 ,  A61K8/64 ,  A61K47/42 ,  C07K1/02 ,  C08J9/28 ,  C08L89/00

CPC分类号： C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K8/987 ,  A61K9/19 ,  A61K47/42 ,  A61K2800/10 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  B01J20/24 ,  C08J9/28 ,  C08J2201/0484 ,  C08J2201/0544 ,  C08J2205/022 ,  C08J2205/028 ,  C08J2389/00 ,  C08L89/00 ,  C11B9/00

摘要： A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm 3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

公开(公告)号：EP2990062A1

公开(公告)日：2016-03-02

申请号：EP14788691.5

申请日：2014-04-18

申请人： Spiber Inc.

发明人： OSAWA, Toshiaki ,  MORITA, Keisuke

IPC分类号： A61L27/00 ,  C07K14/435 ,  C12N15/09

CPC分类号： A61K47/42 ,  A61K9/0087 ,  A61K38/1767 ,  A61L26/008 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/52 ,  A61L27/60 ,  A61L31/145 ,  A61L2430/06 ,  C07K14/43518

摘要： A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass% based on 100 mass% of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.

摘要翻译： 本发明的多肽水凝胶是来自蜘蛛丝蛋白质的多肽的水凝胶，相对于100质量％的多肽水凝胶，水分含量为85.0〜99.9质量％。 水凝胶的制造方法包括：将来自蜘蛛丝蛋白质的多肽溶解在选自（A）二甲基亚砜中的至少一种溶解溶剂中的溶液制备工序; （B）二甲基亚砜与无机盐; 和（C）N，N-二甲基甲酰胺与无机盐反应，得到多肽的溶液; 以及其中在溶液制备步骤中产生的溶液被水溶性溶剂代替的取代步骤。 因此，本发明提供了具有优异的保水性的多肽水凝胶和适用于生物体的多肽水凝胶。

公开(公告)号：EP2940032A1

公开(公告)日：2015-11-04

申请号：EP13867712.5

申请日：2013-12-18

申请人： Spiber Inc.

发明人： OSAWA, Toshiaki ,  MORITA, Keisuke

IPC分类号： C07K1/14 ,  C12P21/02 ,  C07K14/435

CPC分类号： C07K1/145 ,  C07K14/43518 ,  C07K14/78

摘要： The present invention relates to a method for extracting a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a lysate acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to obtain a host cell lysate; and a step in which a diluting solvent is added to the host cell lysate obtained in the lysate acquisition step, an insoluble substance is separated from the obtained dilution, and a supernatant containing the hydrophilic recombinant protein is collected. The hydrophilic recombinant protein has a hydropathy index of 0 or less. The dissolving solvent is an aprotic polar solvent. The diluting solvent is an aqueous solvent.

摘要翻译： 本发明涉及从表达亲水重组蛋白的宿主中提取亲水性重组蛋白的方法。 该方法包括：裂解物获取步骤，其中向表达亲水重组蛋白的宿主中加入溶解溶剂以获得宿主细胞裂解物; 向裂解物获取工序中得到的宿主细胞裂解物中添加稀释溶剂的步骤，从得到的稀释液中分离不溶物质，收集含有亲水性重组蛋白质的上清液。 亲水重组蛋白的亲水指数为0以下。 溶解溶剂是非质子极性溶剂。 稀释溶剂是水性溶剂。

公开(公告)号：EP4382532A1

公开(公告)日：2024-06-12

申请号：EP22853059.8

申请日：2022-08-02

申请人： Spiber Inc.

发明人： KAGATA Hideki ,  SAKATA Kazuki

IPC分类号： C07K14/435 ,  C08J9/28 ,  C12N15/12

CPC分类号： C08J9/28 ,  C07K14/435

摘要： The present disclosure relates to a porous body containing a polypeptide derivative, in which the polypeptide derivative contains a block copolymer containing a first segment containing a polypeptide skeleton, and one or a plurality of second segments that are bonded to the first segment, the polypeptide skeleton is a recombinant polypeptide skeleton or a hydrophobic polypeptide skeleton, and the second segment contains a molecular group having a plasticizing function for the polypeptide skeleton.

公开(公告)号：EP4339183A1

公开(公告)日：2024-03-20

申请号：EP22807545.3

申请日：2022-05-12

申请人： Refine Holdings Co., Ltd. ,  Spiber Inc.

发明人： FUJITA Tomoya ,  IWAFUNE Mitsuhiro ,  SUZUKI Takao ,  TAKEYAMA Tomokiyo ,  ISHIZAWA Hideki ,  ODA Akiyoshi ,  TAKAHASHI Hiroaki ,  OHNO Junya ,  NAKASHIMA Kouji ,  NOUMI Tadashi ,  ISHII Hideto

IPC分类号： C07C51/44 ,  C07C51/48 ,  C07C53/02

摘要： Provided is a method for recovering a carboxylic acid, by which method the carboxylic acid is separated from an aqueous solution containing water and the carboxylic acid and the separated carboxylic acid is obtained as a carboxylic acid anhydride. The method includes a) a first step of bringing the aqueous solution into liquid-liquid contact with a prepared extractant, the prepared extractant containing a component for extracting the carboxylic acid and a diluent to dissolve the component, b) a second step of distilling the prepared extractant that is subjected to the first step, azeotropically distilling the diluent in the prepared extractant and water, separating the distillate into an extractant component layer mainly containing the diluent and an aqueous layer and then discharging the aqueous layer, returning the extractant component layer mainly containing the diluent to the distillation step as reflux, and discharging the prepared extractant containing the carboxylic acid through a bottom of the column; and c) a third step comprising re-distilling the prepared extractant containing the carboxylic acid discharged by the second step, and returning, to the first step, the prepared extractant from which the carboxylic acid and water are removed, the prepared extractant being discharged through a bottom of the column, where the diluent in the prepared extractant have a minimum boiling azeotrope with water.