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11.发明授权
公开(公告)号：EP0091810B1
公开(公告)日：1987-03-25
申请号：EP83302021.7
申请日：1983-04-11
CPC分类号： C12N9/0008 , A23L11/33 , C12Q1/26 , C12R1/38 , C12Y102/03001 , Y10S435/874
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12.发明公开Method for the determination of substrate or enzyme activity 失效Verfahren zur Bestimmung eines Substrats oder der Enzymwirksamkeit。
公开(公告)号：EP0173276A2
公开(公告)日：1986-03-05
申请号：EP85110679.9
申请日：1985-08-26
摘要： 57 The determination of a substrate or an activity of an enzyme in a sample containing ammonia by using a reaction system which forms ammonia can be performed by decomposing ammonia in the sample with an ammonia-decomposing reagent system, adding a reagent system necessary for the formation of ammonia from the substrate or the substrate for the enzyme to form ammonia and determining the formed ammonia.
摘要翻译： 通过使用形成氨的反应体系测定含有氨的样品中的底物或酶的活性可以通过用氨分解试剂系统分解样品中的氨来进行，添加形成氨所需的试剂系统 来自底物的氨或用于酶的底物以形成氨并测定形成的氨。
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13.发明公开Test composition for the determination of triglycerides and its use 失效Reagenzzusammensetzung zur Bestimmung von Triglyceriden und deren Verwendung。
公开(公告)号：EP0010296A1
公开(公告)日：1980-04-30
申请号：EP79104011.6
申请日：1979-10-17
发明人： Nagai, Toshiaki , Terada, Osamu , Uwajima, Takayuki , Mihara, Akira , Aisaka, Kazuo , Akita, Hiroko , Shimizu, Yoshiaki
摘要： Triglycerides in a sample are quantitatively determined using a test composition having a system for converting the triglycerides to fatty acids and glycerol, a system for oxidizing the glycerol, and a system for determining the amount of oxygen consumed or the amount of hydrogen peroxide or glyceraldehyde formed in the oxidation of the glycerol.
摘要翻译： 使用具有将甘油三酯转化为脂肪酸和甘油的系统的测试组合物，用于氧化甘油的系统和用于测定消耗的氧气量或形成的过氧化氢或甘油醛的量的系统来定量测定样品中的甘油三酸酯 在甘油的氧化中。
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公开(公告)号：EP0730028A1
公开(公告)日：1996-09-04
申请号：EP96200349.7
申请日：1991-10-09
CPC分类号： C12N9/0006 , C12Q1/60
摘要： A novel cholesterol oxidase having a high substrate affinity for cholesterol, and a wide working pH range is provided, as well as a method for the production of said cholesterol oxidase which comprises culturing a microorganism belonging to the genus Escherichia which carries a recombinant DNA comprising a DNA derived from Brevibacterium sterolicum and defined in the Sequence Listing by SEQ ID NO: 1.
摘要翻译： 提供了对胆固醇具有高底物亲和性和宽工作pH范围的新型胆固醇氧化酶，以及生产所述胆固醇氧化酶的方法，其包括培养属于埃希氏杆菌属的微生物，其携带重组DNA，所述重组DNA包含 衍生自短杆菌（Brevibacterium sterolicum）并在序列表中由SEQ ID NO：1定义的DNA
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公开(公告)号：EP0452112A1
公开(公告)日：1991-10-16
申请号：EP91303164.7
申请日：1991-04-10
CPC分类号： C12N9/0006 , C12Q1/60
摘要： A novel cholesterol oxidase is disclosed having a high substrate affinity, high activity and operable over a wide pH range, including acid value. The enzyme is produced in low yield by microorganisms of the species Brevibacterium sterolicum . Improved yields are obtained by transforming microorganisms of the species E.coli with the DNA from Brevibacterium sterolicum and which encodes the novel enzyme.
摘要翻译： 公开了一种新颖的胆固醇氧化酶，其具有高的底物亲和力，高活性，并且在宽的pH范围（包括酸值）下可操作。 该酶由不育菌短杆菌的微生物以低产率产生。 通过用来自立枯丝杆菌的DNA转化大肠杆菌物种的微生物并编码新的酶来获得提高的产量。
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公开(公告)号：EP0164754B1
公开(公告)日：1991-03-06
申请号：EP85107297.5
申请日：1985-06-13
发明人： Uwajima, Takayuki , Aisaka, Kazuo
CPC分类号： C12N9/88 , Y10S435/849
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17.发明授权Process for producing neuraminidase and method and reagent for the quantitative determination of a sialic acid-containing substance using neuraminidase 失效生产神经酰胺糖激酶的方法及使用神经酰胺糖激酶定量测定含磷酸的物质的方法和试剂
公开(公告)号：EP0133694B1
公开(公告)日：1990-07-25
申请号：EP84109221.6
申请日：1984-08-03
发明人： Uwajima, Takayuki , Aisaka, Kazuo
CPC分类号： C12Y302/01018 , C12N9/24 , C12N9/2402 , C12Q1/34 , C12R1/29 , Y10S435/867 , Y10S435/868
摘要： Neuraminidase is produced by culturing a neuraminidase producing microorganism belonging to the genus Micromonospora. The neuraminidase is useful for the quantitative determination of a sialic acid-containing substance in a sample.
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公开(公告)号：EP0297533A2
公开(公告)日：1989-01-04
申请号：EP88110366.7
申请日：1988-06-29
CPC分类号： C12N9/80 , C12Q1/34 , G01N2333/948 , Y10S435/814 , Y10S435/815
摘要： A novel acetylpolyamine amidohydrolase is described. The enzyme specifically hydrolyzes the amino bond in acetylputrescine, acetylcadaverine, acetylspermidine and acetylspermine with strong substrate affinity. The enzyme is preferably produced by culturing a microorganism belonging to the genus Mycoplana, and is used in the quantitative determination of polyamine contained in a living body sample, which is useful for cancer diagnosis.
摘要翻译： 描述了一种新型的乙酰多胺酰胺水解酶。 该酶特异性水解乙酰肌氨酸，乙酰胆碱，乙酰精胺和乙酰精胺中的氨基键，具有强的底物亲和力。 该酶优选通过培养属于Mycoplana属的微生物来生产，并用于定量测定活体样品中含有的多胺，其可用于癌症诊断。
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公开(公告)号：EP0164754A1
公开(公告)日：1985-12-18
申请号：EP85107297.5
申请日：1985-06-13
发明人： Uwajima, Takayuki , Aisaka, Kazuo
CPC分类号： C12N9/88 , Y10S435/849
摘要： The present invention provides a process for producing N-acetylneuraminate lyase using a microorganism constructed by recombinant DNA techniques.
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