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公开(公告)号：EP1153279A2
公开(公告)日：2001-11-14
申请号：EP00907121.8
申请日：2000-02-03
IPC分类号： G01N15/12
CPC分类号： G01N15/1227 , H03B5/1218 , H03B5/1228 , H03B5/124 , H03B5/1296
摘要： A junction field effect transistor (JFET) RF oscillator-detector circuit generates an RF signal for an apparatus for conducting electrical measurements of particles contained in a carrier fluid passing through an aperture in a cytometer flow cell. The JFET oscillator includes a plurality of parallel-coupled JFETs having respectively different VDS vs. IDS characteristics, that are biased to operate at square law detection regions of their respective VDS vs. IDS characteristics. One JFET operates in Class C mode, while the other operates in Class AB mode. An RF resonant circuit is electrically coupled to the JFETs and to the measurement cell, and is operative to establish the frequency of an RF field applied to the measurement cell. An RF load change detection circuit is coupled to the RF resonator circuit and is operative to detect an RF load change associated with a modification of the RF field as a result of a particle within the measurement cell aperture.
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12.发明公开
公开(公告)号：EP1099105A1
公开(公告)日：2001-05-16
申请号：EP99931885.0
申请日：1999-06-25
发明人： BURR, Robert , LARY, Todd , FRAZIER, Erich , MIRANDA, Osvaldo, E.
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1406
摘要： A droplet travel path monitoring mechanism for a flow cytometer is operative to adjust the droplet break-off point back to an initially calibrated spatial location, in the event of the departure from calibrated timing of gaps in the unsorted fluid droplet stream that have been created by the deflection of charged droplets. In addition, the flow cytometer is operative to monitor prescribed characteristics of deflected droplet streams, and to controllably adjust drop sorting deflection parameters, so as to maintain the deflected travel path of sorted droplets coincident with the opening into a sorted droplet collection container, thereby maximizing collection of all sorted droplets.
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公开(公告)号：EP1032819A1
公开(公告)日：2000-09-06
申请号：EP98958559.1
申请日：1998-11-13
发明人： LI, Yi , LI, Jing , YOUNG, Carole
CPC分类号： G01N33/80 , G01N15/12 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666 , Y10T436/25125
摘要： A method is provided for differentiation of reticulocytes. In addition, the method provides for a concurrent differentiation of leukocyte subpopulations in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing reticulocytes in a flow cell by optical analysis. A concurrent differentiation of reticulocytes and leukocytes can be performed using electronic and optical analysis. Th electronic and optical analysis includes light scatter and impedance measurements. This method does not require the use of nuclear stain for differentiation of reticulocytes.
摘要翻译： 提供了分化网织红细胞的方法。 此外，该方法通过合适的电子和光学测量提供了血细胞样品中白细胞亚群的同时分化。 该方法包括将血细胞样品暴露于试剂系统以溶解成熟的红血细胞并随后通过光学分析分析流动池中的网状细胞。 使用电子和光学分析可以进行网状细胞和白细胞的同时分化。 电子和光学分析包括光散射和阻抗测量。 该方法不需要使用核染色来分化网织红细胞。
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14.发明公开
公开(公告)号：EP1023601A2
公开(公告)日：2000-08-02
申请号：EP98953228.8
申请日：1998-10-01
IPC分类号： G01N33/543
CPC分类号： G01N33/56972 , G01N33/5434 , G01N33/553 , G01N2446/20 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
摘要： A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.
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15.发明公开
公开(公告)号：EP0981747A1
公开(公告)日：2000-03-01
申请号：EP98922261.7
申请日：1998-05-14
发明人： SIIMAN, Olavi , BURSHTEYN, Alexander , MYLVAGANAM, Ravindra , RAYNOR, Robert , ROTH, Patricia , SMITH, Cecilia , WILKINSON, Julie
IPC分类号： G01N33/533 , G01N33/566 , G01N33/569
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye.
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16.发明公开
公开(公告)号：EP0941464A2
公开(公告)日：1999-09-15
申请号：EP97950713.0
申请日：1997-11-25
CPC分类号： G01N15/1459 , G01N2015/0073 , G01N2015/1037 , G01N2015/1495 , G01N2015/1497 , G01N2021/4716
摘要： The present invention provides an apparatus and method to determine individual red blood cell shape within a flow cytometric aperture. Cell shape is determined by measuring the asymmetry of a light scatter pattern produced by laser illumination of the cell within the aperture by light scatter detectors which collect light from specific angular regions of light scatter, and analyzing the asymmetry of the light scatter patterns to determine the individual red blood cell shape. Embodiments of the invention further provide for the determination of red blood cell shape by measuring the asymmetry of a light scatter pattern and measuring electrical resistance of the red blood cell.
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公开(公告)号：EP1131629B1
公开(公告)日：2009-01-14
申请号：EP99970736.7
申请日：1999-09-27
发明人： HORTON, Allan , WYATT, James, L.
CPC分类号： G01N33/5094 , G01N15/12 , G01N15/1459 , G01N33/721 , G01N33/80 , G01N2015/1402 , G01N2015/1477 , Y10T436/10 , Y10T436/101666 , Y10T436/107497
摘要： A reagent composition and method is provided for identification of reticulated cells from other cell types in a blood cell sample by using optical measurement. More particularly, the reagent composition comprises a nucleic acid dye and sphering agent. More particularly, the method enables the identification of reticulated platelets and reticulated erythrocytes. In addition, the method provides for a concurrent determination of cell by cell hemoglobin of the reticulated erythrocytes and mature erythrocytes in the blood cell sample.
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18.发明公开APPARATUS AND METHOD FOR ANALYSING A LIQUID IN A CAPILLARY TUBE OF A HEMATOLOGY INSTRUMENT 有权装置和方法分析液态血液学的仪器的毛细管
公开(公告)号：EP1572000A2
公开(公告)日：2005-09-14
申请号：EP03774493.5
申请日：2003-09-25
IPC分类号： A61B8/00
CPC分类号： G01N33/491 , G01N11/04 , G01N15/05 , G01N29/024 , G01N2291/02466 , G01N2291/02818 , G01N2291/02836
摘要： An apparatus and method for determining the density and fluid-type of a fluid flowing in a capillary tube, the velocity and viscosity of a blood sample flowing in a capillary tube, the erythrocyte sedimentation rate (ESR) of a blood sample after flow has been brought to an abrupt stop in a capillary tube, and/or the zeta sedimentation rate (ZSR) of a blood sample after flow has been brought to an abrupt stop in a capillary tube. These measurements are accomplished by directing a waveform pulse, such as an ultrasound pulse, at a pre-determined frequency transversely across the capillary tube and sample fluid, and by determining the flight of time of the pulse through the capillary tube and sample fluid and/or the Doppler shift of the echo signals reflecting off cells moving forwardly or transversely within a flowing, or stationary, blood sample.
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19.发明授权
公开(公告)号：EP1303751B1
公开(公告)日：2005-08-10
申请号：EP01933047.1
申请日：2001-05-04
发明人： DEKA, Chiranjit , LEE, Song, Y. , SHEN, Gene, G.-Y. , SZYDLO, Stephen , TSAY, Tsong-Tseh , GUPTA, Ravinder
CPC分类号： C09B23/02 , G01N1/30 , G01N21/47 , G01N21/6428 , G01N2001/302 , Y10S436/80 , Y10T436/10 , Y10T436/101666
摘要： The dyes of the present invention are useful for many purposes that include markers or tags for detecting the presence of a molecule or compound to which they are bound. The dyes may be either red-excitable or blue-excitable. The dyes of the invention are particularly well suited for staining of nucleic acids. For example, these dyes are particularly suitable for staining of RNA in reticulocytes. In another exemplary application, these dyes are suitable for staining DNA in nucleated red blood cells. Typically, when used in staining of nucleic acids, the dyes are formulated into reagent solutions. In addition, the invention provides compositions and methods for facilitating rapid transport of dye molecules through a cell membrane. Such rapid staining requires that a sample be contacted with a dye composition of the invention in the presence of at least one surfactant and optionally, a sulfonic acid or a salt thereof.
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20.发明授权
公开(公告)号：EP0948639B1
公开(公告)日：2005-08-10
申请号：EP97913899.7
申请日：1997-10-29
发明人： KUYLEN, Nazle , CREWS, Harold, R. , JAFFE, Gerald, E. , BARCELON, Maria , LUCAS, Francis, J. , GARCIA, Nancy, M.
IPC分类号： C12Q1/00 , C12Q1/04 , C12Q1/26 , C12Q1/37 , C12Q1/44 , C12Q1/42 , C12Q1/34 , G01N15/14 , G01N33/50 , G01N21/64
CPC分类号： C12Q1/34 , C12Q1/26 , C12Q1/37 , G01N33/574 , G01N33/582 , G01N2333/95 , G01N2333/96413 , G01N2333/96466 , G01N2333/96469 , G01N2800/52
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