公开(公告)号：EP1914238A3

公开(公告)日：2008-11-05

申请号：EP07023766.4

申请日：1996-04-03

申请人： Wisconsin Alumni Research Foundation

发明人： Schwartz, David

IPC分类号： C07H19/00 ,  C07H21/00 ,  C12Q1/68 ,  C12M1/00 ,  G01N27/447

CPC分类号： C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  G01N2015/0038 ,  G01N2015/1043 ,  G01N2015/105 ,  G01N2015/149 ,  G01N2015/1495 ,  C12Q2565/601 ,  C12Q2527/125

摘要： The present invention conerns a method of preparing an individual elongated nucleic acid molecule fixed onto a solid planar surface, comprising depositing the nucleic acid molecule onto a planar glass surface coated with a positively charged substance that increases the electrostatic interaction between the nucleic acid molecule and the surface, and which reproducibly elongates and fixes the nucleic acid molecule to the surface such that the single nucleic acid spans 20% to 60% of its curvilinear contour length, wherein the charge density is sufficient to maintain the nucleic acid molecule in an elongated state while allowing for a small degree of relaxation, and wherein the small degree of relaxation yields a gap of 0.5 µm to 5.0 µm when the elongated nucleic acid molecule is cut, and wherein the elongated fixed nucleic acid molecule remains individually manipulable and accessible for enzymatic and/or hybridization reactions.

公开(公告)号：EP1402245A4

公开(公告)日：2006-03-22

申请号：EP02737289

申请日：2002-05-31

申请人： US GENOMICS INC

发明人： CHAN EUGENE Y ,  GLEICH LANCE C ,  WELLMAN PARRIS S

IPC分类号： B01F5/06 ,  B01F13/00 ,  B01L3/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/28 ,  G01N15/14 ,  G01N21/64 ,  G01N21/78 ,  G01N27/447 ,  G01N33/44 ,  G01N33/483

CPC分类号： B01L3/502761 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N1/286 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1463 ,  G01N33/442 ,  G01N2015/1075 ,  G01N2015/1493 ,  G01N2015/1495 ,  Y10T436/13 ,  Y10T436/143333

摘要： In a method and apparatus for determining the velocity of an elongated polymeric molecule, the molecule (105) is moved relative to one or more detection stations (101,.102, 103). Time correlated detection signals are used to determine velocity, length, and distance between landmarks on the molecule (105).

公开(公告)号：EP0391674A2

公开(公告)日：1990-10-10

申请号：EP90303579.8

申请日：1990-04-03

申请人： NEW YORK UNIVERSITY

发明人： Schwartz, David C.

IPC分类号： G01N27/447

CPC分类号： G01N15/02 ,  C12Q1/683 ,  C12Q1/6841 ,  G01N27/44721 ,  G01N27/44773 ,  G01N2015/0288 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  Y10S435/82 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S977/853 ,  Y10T436/143333

摘要： A method for observing and determining the size of individual particles and for determining the weight distribution of a sample containing particles of varying size, which involves placing a deformable or nondefor- mable particle in a medium, subjecting the particle to an external force, thereby causing conformational and/or positional changes, and then measuring these changes. Preferred ways to measure conformational and positional changes include: (1) determining the rate at which a deformable particle returns to a relaxed state after termination of the external force, (2) determining the rate at which a particle becomes oriented in a new direction when the direction of the perturbing force is changed, (3) determining the rate at which a particle rotates, (4) measuring the length of a particle, particularly when it is at least partially stretched, or (5) measuring at least one diameter of a spherical or ellipsoidal particle. Measurements of relaxation, reorientation, and rotation rates, as well as length and diameter can be made using a light microscope connected to an image processor. Particle relaxation, reorientation and rotation also can be determined using a microscope combined with a spectroscopic device. The invention is' particularly useful for measuring polymer molecules, such as nucleic acids, and can be used to determine the size and map location of restriction digests. Breakage of large polymer molecules mounted on a microscope slide is prevented by condensing the molecules before mounting and unfolding the molecules after they have been placed in a matrix.

摘要翻译： 一种用于观察和确定单个颗粒的尺寸并确定含有不同大小的颗粒的样品的重量分布的方法，其包括将可变形或不可变形的颗粒放置在介质中，使颗粒受到外力，由此引起构象和 /或位置变化，然后测量这些变化。 测量构象和位置变化的优选方法包括：（1）确定可变形颗粒在外力终止后返回到松弛状态的速率，（2）确定颗粒在新方向上定向的速率，当 扰动力的方向改变，（3）确定颗粒旋转的速度，（4）测量颗粒的长度，特别是当其至少部分拉伸时，或（5）测量至少一个直径的 球形或椭圆形颗粒。 可以使用连接到图像处理器的光学显微镜来进行放松，重新定向和旋转速率以及长度和直径的测量。 也可以使用与分光装置结合的显微镜来确定粒子松弛，重新取向和旋转。 本发明对于测量聚合物分子（例如核酸）特别有用，并且可用于确定限制性消化物的大小和图谱位置。 通过在将分子置于基质中之前将分子凝结并解离之前将分子凝聚来防止在显微镜载片上安装的大分子的破裂。

公开(公告)号：EP2216639A4

公开(公告)日：2014-01-15

申请号：EP08853229

申请日：2008-10-28

申请人： KONICA MINOLTA OPTO INC

发明人： ICHITANI SHUJI ,  TOYAMA OSAMU

IPC分类号： G01N11/04 ,  G01N33/49

CPC分类号： G01N11/04 ,  G01F3/36 ,  G01N15/1463 ,  G01N33/49 ,  G01N2015/1075 ,  G01N2015/1495 ,  G06T7/215 ,  G06T2207/30104

摘要： Deformability of blood cells is quantified in a short time. A blood fluidity measurement apparatus is provided with a TV camera (6) which photographs a stream of blood in either two areas of the internal area (A), entrance area (B), and exit area (C) of a gate (30), an image processing part (7) which calculates the velocity of the blood cells contained in the blood from the image taken by the TV camera (6), and a deformability calculation means (81) which calculates the deformability of the blood cells a blood fluidity from the velocity.

摘要翻译： 在短时间内量化血细胞的变形性。 血液流动性测量装置设置有电视摄像机（6），其在门（30）的内​​部区域（A），入口区域（B）和出口区域（C）的两个区域中拍摄血液流， ，计算由TV摄像机（6）拍摄的图像中包含在血液中的血细胞的速度的图像处理部（7），以及计算血细胞变形性的变形能力计算单元（81） 流速从速度。

公开(公告)号：EP2219020A1

公开(公告)日：2010-08-18

申请号：EP08853723.8

申请日：2008-10-28

申请人： Konica Minolta Opto, Inc.

发明人： ICHITANI, Shuji ,  TAKAMA, Masaaki ,  MURAYAMA, Takanori

IPC分类号： G01N11/04 ,  G01N33/49

CPC分类号： G01N11/06 ,  G01N11/08 ,  G01N35/1097 ,  G01N2011/008 ,  G01N2015/0073 ,  G01N2015/0092 ,  G01N2015/1075 ,  G01N2015/1495 ,  G06T7/0012 ,  G06T2207/10016 ,  G06T2207/30104

摘要： Blood fluidity is measured in a short time. A blood fluidity measurement system (1), which measures blood fluidity by flowing blood into a channel, is equipped with a TV camera (6) which photographs the blood stream in the channel and an image processing part (7) which detects the state of the blood stream in the channel as blood fluidity from the image taken by the TV camera (6).

摘要翻译： 血液流动性在短时间内测量。 通过将血液流入通道来测量血液流动性的血液流动性测量系统（1）配备有摄像通道中的血流的电视摄像机（6）和检测通道中的血液的状态的图像处理部（7） 通道中的血流作为由电视摄像机（6）拍摄的图像的血液流动性。

公开(公告)号：EP2216639A1

公开(公告)日：2010-08-11

申请号：EP08853229.6

申请日：2008-10-28

申请人： Konica Minolta Opto, Inc.

发明人： ICHITANI, Shuji ,  TOYAMA, Osamu

IPC分类号： G01N11/04 ,  G01N33/49

CPC分类号： G01N11/04 ,  G01F3/36 ,  G01N15/1463 ,  G01N33/49 ,  G01N2015/1075 ,  G01N2015/1495 ,  G06T7/215 ,  G06T2207/30104

摘要： Deformability of blood cells is quantified in a short time. A blood fluidity measurement apparatus is provided with a TV camera (6) which photographs a stream of blood in either two areas of the internal area (A), entrance area (B), and exit area (C) of a gate (30), an image processing part (7) which calculates the velocity of the blood cells contained in the blood from the image taken by the TV camera (6), and a deformability calculation means (81) which calculates the deformability of the blood cells a blood fluidity from the velocity.

公开(公告)号：EP1402245A1

公开(公告)日：2004-03-31

申请号：EP02737289.5

申请日：2002-05-31

申请人： U.S. Genomics, Inc.

发明人： CHAN, Eugene, Y. ,  GLEICH, Lance, C. ,  WELLMAN, Parris, S.

IPC分类号： G01N21/64

CPC分类号： B01L3/502761 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N1/286 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1463 ,  G01N33/442 ,  G01N2015/1075 ,  G01N2015/1493 ,  G01N2015/1495 ,  Y10T436/13 ,  Y10T436/143333

摘要： In a method and apparatus for determining the velocity of an elongated polymeric molecule, the molecule (105) is moved relative to one or more detection stations (101,.102, 103). Time correlated detection signals are used to determine velocity, length, and distance between landmarks on the molecule (105).

公开(公告)号：EP3394939A1

公开(公告)日：2018-10-31

申请号：EP16880115.7

申请日：2016-12-22

申请人： Canon U.S. Life Sciences, Inc. ,  University of Maryland, College Park

发明人： SCARCELLI, Giuliano ,  ZHANG, Jitao ,  FIORE, Antonio ,  KIM, Hanyoup

IPC分类号： H01S3/00 ,  H01S3/30 ,  H01S3/108

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1495 ,  G01N2021/638

摘要： The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

公开(公告)号：EP2912434A4

公开(公告)日：2016-06-29

申请号：EP13871771

申请日：2013-10-18

申请人： UNIV CALIFORNIA

发明人： DI CARLO DINO ,  GOSSETT DANIEL R ,  TSE HENRY T K ,  CHUNG ARAM

IPC分类号： G01N15/14 ,  G01B11/24 ,  G01N21/64

CPC分类号： G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N2021/6439 ,  G06K9/4604 ,  G06T7/0004 ,  G06T7/0016

摘要： A system for deforming and analyzing particles includes a substrate defining an inlet, and an outlet; a fluidic pathway fluidly coupled to the inlet and the outlet and defining a delivery region upstream of a deformation region configured to deform particles, wherein the fluidic pathway comprises a first branch configured to generate a first flow, and a second branch configured to generate a second flow that opposes the first flow, wherein an intersection of the first flow and the second flow defines the deformation region; a detection module including a sensor configured to generate a morphology dataset characterizing deformation of the particles, and a photodetector configured to generate a fluorescence dataset characterizing fluorescence of the particles; and a processor configured to output an analysis of the plurality of particles based at least in part on the deformation dataset and the fluorescent dataset for the plurality of particles.

公开(公告)号：EP2619545A2

公开(公告)日：2013-07-31

申请号：EP11827272.3

申请日：2011-09-16

申请人： The Regents of the University of California

发明人： DI CARLO, Dino ,  GOSSETT, Daniel R. ,  TSE, Henry T.K.

IPC分类号： G01N15/10 ,  G01N21/29 ,  G01N33/48 ,  G01N35/08

CPC分类号： G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G06F19/10

摘要： A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.