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    AUXINIC ANALOGUES OF INDOLE-3-ACETIC ACID
    11.
    发明公开
    AUXINIC ANALOGUES OF INDOLE-3-ACETIC ACID 失效
    AUXIN ANALOGE

    公开(公告)号：EP0866850A4

    公开(公告)日：2000-11-22

    申请号：EP96943547

    申请日：1996-11-29

    申请人： LIFE TECHNOLOGIES INC

    发明人： LIN JHY-JHU ,  LAN JIANQING ,  ASSAD-GARCIA NACYRA

    IPC分类号： A01H4/00 ,  A01N43/38 ,  C07D209/18 ,  C12N5/00 ,  C12N15/82 ,  A01N33/00 ,  C07D209/04

    CPC分类号： C12N5/0025 ,  A01H4/008 ,  A01N43/38 ,  C07D209/18 ,  C12N15/8201 ,  C12N15/8205

    摘要： The present invention provides compounds and compositions capable of stimulating plant growth, regeneration of plant cells and tissues, and transformation of plant cells and tissues, comprising mono- and multi-substituted auxinic analogues of indole-3-acetic acid (IAA) comprising substituent groups such as halo-, alkyl-, alkoxy-, acyl-, acylamido- and acyloxy-groups. The invention relates to a method of using such mono- and multi-substituted auxinic analogues of IAA to affect growth, regeneration or transformation in monocotyledonous and dicotyledonous plants, as well as in transgenic plant tissues. The invention also contemplates the use of these auxinic IAA analogues in the presence of other plant growth regulators, such as cytokinin, etc., to enhance plant growth.

    摘要翻译： 本发明提供了能够刺激植物生长，植物细胞和组织再生以及植物细胞和组织转化的化合物和组合物，其包含单取代和多取代的包含取代基的吲哚-3-乙酸（IAA）的生长素类似物 例如卤代，烷基 - ，烷氧基 - ，酰基 - ，酰基酰氨基 - 和酰氧基。 本发明涉及使用IAA的单取代和多取代的生长素类似物来影响单子叶植物和双子叶植物以及转基因植物组织中的生长，再生或转化的方法。 本发明还考虑在其它植物生长调节剂如细胞分裂素等存在下使用这些生长素IAA类似物以增强植物生长。

    DRY POWDER CELLS AND CELL CULTURE REAGENTS AND METHODS OF PRODUCTION THEREOF
    13.
    发明公开
    DRY POWDER CELLS AND CELL CULTURE REAGENTS AND METHODS OF PRODUCTION THEREOF 失效
    干电池和方法生产的细胞培养试剂和

    公开(公告)号：EP1012234A1

    公开(公告)日：2000-06-28

    申请号：EP98908542.8

    申请日：1998-02-17

    申请人： Life Technologies, Inc.

    发明人： FIKE, Richard ,  WHITFORD, William ,  BIDDLE, William

    IPC分类号： C12N5/00 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20

    CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

    摘要： The present invention relates generally to nutritive medium, medi0m supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.

    HIGH FIDELITY POLYMERASES AND USES THEREOF
    14.
    发明公开
    HIGH FIDELITY POLYMERASES AND USES THEREOF 有权
    高灵敏度聚合酶和用途

    公开(公告)号：EP1012161A1

    公开(公告)日：2000-06-28

    申请号：EP98943434.5

    申请日：1998-08-28

    申请人： LIFE TECHNOLOGIES, INC.

    发明人： YANG, Shuwei ,  CHATTERJEE, Deb, K.

    IPC分类号： C07H21/04 ,  C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1247 ,  C12N9/1252

    摘要： The present invention relates to a DNA and RNA polymerases which have increased fidelity (or reduced misincorporation rate). In particular, the invention relates to a method of making such polymerases by modifying or mutating the nucleotide binding domain of the polymerase (e.g., the O-helix). The invention also relates to DNA molecules containing the genes encoding the polymerases of the invention, to host cells containing such DNA molecules and to methods to make the polymerases using the host cells. The polymerases are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

    CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF
    15.
    发明公开
    CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF 失效
    CAP-结合​​区的人真核蛋白的合成起始因子EIF-4E及其应用

    公开(公告)号：EP0975652A1

    公开(公告)日：2000-02-02

    申请号：EP97942384.5

    申请日：1997-09-02

    申请人： LIFE TECHNOLOGIES, INC.

    发明人： GOSS, Dixie, J. ,  FRIEDLAND, Diana

    IPC分类号： C07H21/04 ,  C07K1/04 ,  C07K1/14 ,  C07K1/36 ,  C07K2/00 ,  C07K4/00 ,  C07K5/02 ,  C07K7/02

    CPC分类号： C07K14/4705

    摘要： Binding of eIF-4E to the 5'm7G cap structure of eukaryotic mRNA signals the initiation of protein synthesis. In the present invention, the binding site cap-binding domain of eIF-4E was identified as the region containing the sequence Trp?113 to Arg122¿. Thus, in a first preferred embodiment the present invention provides a truncated peptide of the cap binding protein consisting essentially of the mRNA cap-binding domain. Specifically, the invention concerns eIF-4E cap binding peptide and its variants. In additional preferred embodiments, the invention provides an isolated nucleic acid molecule which encodes a truncated peptide of the eIF-4E protein consisting essentially of the mRNA cap-binding domain of eIF-4E; a recombinant vector and host cell comprising the above-described nucleic acid molecule; and a recombinant peptide produced thereby. The invention further provides the truncated or recombinant peptides immobilized on a solid support, a resin comprising the truncated or recombinant peptides immobilized on a solid support, methods and kits for isolating a capped mRNA molecule, and methods and kits for producing a full-length cDNA molecule.

    STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING
    17.
    发明公开
    STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 失效
    STABLE PREPARATIONS作者核酸扩增和测序方法USE

    公开(公告)号：EP0942917A1

    公开(公告)日：1999-09-22

    申请号：EP97938279.0

    申请日：1997-08-14

    申请人： Life Technologies, Inc.

    发明人： RASHTCHIAN, Ayoub ,  SOLUS, Joseph

    IPC分类号： G01N33 ,  C12N15 ,  C12P21 ,  C12Q1

    CPC分类号： C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2527/149 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： This invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperature above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction, as exemplified in the Figure) and sequencing (e.g., by dideoxy or 'Sanger' sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.