公开(公告)号：EP1842920B1

公开(公告)日：2010-01-13

申请号：EP07109353.8

申请日：2004-09-23

申请人： UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

发明人： Stafford, Darrel ,  Li, Tao

IPC分类号： C12N15/68 ,  C12N9/04

CPC分类号： C12N15/1137 ,  C12N9/0006 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

公开(公告)号：EP1444367B1

公开(公告)日：2009-02-11

申请号：EP02802983.3

申请日：2002-11-11

申请人： Evonik Degussa GmbH

发明人： MAY, Oliver ,  LIEBETON, Klaus ,  ECK, Jürgen

IPC分类号： C12R1/19 ,  C12N15/70 ,  C12N15/68

CPC分类号： C12R1/19 ,  C12N15/68 ,  C12N15/70 ,  C12P13/04 ,  C12P21/02 ,  C12P41/006

摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-inducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, expression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.

公开(公告)号：EP1507863B1

公开(公告)日：2006-12-27

申请号：EP03752862.7

申请日：2003-05-19

申请人： Cobra Biologics Limited

发明人： HANAK, Julian, Cobra Therapeutics Limited ,  CRANENBURGH, Rocky, Cobra Therapeutics Limited ,  GORMAN, Scott, Pharmaventures Limited

IPC分类号： C12N15/68 ,  C12N15/74 ,  C12N15/64 ,  A61K39/112 ,  A61K39/02 ,  A61K35/74 ,  A61K48/00 ,  C12N1/36 ,  C12R1/42

CPC分类号： C12N1/36 ,  A61K48/00 ,  A61K2035/11 ,  A61K2039/522 ,  C12N15/64 ,  C12N15/68 ,  C12N15/74 ,  Y02A50/484

摘要： This invention provides in vivo methods of maintaining a plasmid within a host cell, which is in turn within a recipient organism. In particular it relates to methods of maintaining a plasmid containing a gene useful in therapy, especially vaccination. The invention is based on modified host cells, suitable for in vivo therapeutic use, that may be used to express a therapeutically useful plasmid-borne gene, using a plasmid maintenance system that does not require the use of a plasmid-borne dominant selectable marker, but rather utilises a system of repressor titration. Methods of selecting host cells comprising the plasmid and host cells for use in these methods are also provided.

公开(公告)号：EP1579001A2

公开(公告)日：2005-09-28

申请号：EP03722001.9

申请日：2003-04-30

申请人： UNIVERSITY OF SOUTH FLORIDA

发明人： MOHAPATRA, Shyam S. ,  BEHERA, Aruna K.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N15/68 ,  C07H21/02 ,  C07H21/04 ,  A01N43/04

CPC分类号： A61K38/45 ,  A61K38/217 ,  A61K38/51 ,  A61K48/00 ,  A61K48/005 ,  C12N9/1241 ,  C12N2710/10343 ,  C12N2799/022

摘要： The subject invention concerns a method of inhibiting an RNA virus infection within a patient by increasing the amount of 2-5 oligoadenylate (2-5 AS) activity within the patient. Preferably, the preventive and therapeutic methods of the present invention involve administering a nucleotide encoding 2-5 AS, or at least one catalytically active fragment thereof, such as the p40, p69, p100 subunits to a patient in need thereof. The present inventors have determined that overexpression of 2-5AS causes a reduction in epithelial cell damage, reduction in infiltration of mononuclear cells in the peribronchiolar and perivascular regions, and reduction in thickening of the septa in the lungs. Levels of chemokines, such as MIP1-α, are also reduced upon overexpression of 2-5AS. The subject invention also pertains to pharmaceutical compositions containing a nucleotide sequence encoding 2-5AS and pharmaceutically acceptable carrier, as well as vectors for delivery of the 2-5AS nucleotide sequence.

公开(公告)号：EP1523498A1

公开(公告)日：2005-04-20

申请号：EP03760733.0

申请日：2003-06-18

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE ,  Centre National De La Recherche Scientifique

发明人： GONDRY, Muriel ,  GENET, Roger ,  LAUTRU, Sylvie ,  PERNODET, Jean-Luc

IPC分类号： C07K14/36 ,  C12P21/04 ,  C12N15/68 ,  C12Q1/68

CPC分类号： C07K14/36

摘要： The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6- substituted by α,β-unsaturated amino acid side chains.

公开(公告)号：EP0283475B1

公开(公告)日：1994-05-18

申请号：EP86906826.2

申请日：1986-11-24

申请人： BIOGEN, INC.

发明人： ERNST, Joachim, F. ,  SCHMEISSNER, Ursula

IPC分类号： C12N15/81 ,  C12N1/19 ,  C12N15/68

CPC分类号： C07K14/65 ,  C07K14/395 ,  C07K2319/02 ,  C12N15/69 ,  C12N15/81

摘要： Improved secretion of heterologous proteins by hosts such as yeast by using promoters of at most intermediate strength with heterologous DNA secretion signal sequences. A promoter of at most intermediate strength, such as the actin (ACT) or iso-l-cytochrome c (CYCl) promoter in S. cerevisiae is operatively linked to a DNA signal sequence, such as the MFalpha) signal sequence. A DNA sequence for a selected protein, such as somatomedin C (SMC), tissue plasminogen activator (TPA) or tumor necrosis factor (TNF), may be operatively linked to the DNA signal sequence.

公开(公告)号：EP0242359B1

公开(公告)日：1993-07-21

申请号：EP86900017.4

申请日：1985-12-04

申请人： BIOTECHNOLOGY AUSTRALIA PTY. LTD.

发明人： CLARK, Susan, Joy ,  CAHILL, Ann, Denise

IPC分类号： A61K39/108 ,  C07G17/00 ,  C07K15/12 ,  C12N1/20 ,  C07K15/00 ,  C12P19/34 ,  C12P21/00 ,  C12N15/68

CPC分类号： C12N15/68 ,  A61K39/00 ,  C07K14/245

摘要： A new method of cloning large fragments of DNA by inserting the fragment into a cloning vehicle which also contains a high copy plasmid and a partition locus. The process of the invention is particularly useful for producing 987P fimbrial proteins by transformed E. coli, for incorporation into vaccines for the prevention and treatment of scours in piglets. The process is also useful as a tool in the creation of DNA libraries for eukaryotes.

公开(公告)号：EP0180899B1

公开(公告)日：1992-01-15

申请号：EP85113735.6

申请日：1985-10-29

申请人： PHILLIPS PETROLEUM COMPANY

发明人： Cregg, James Michael

IPC分类号： C12N15/68 ,  C12N15/81

CPC分类号： C12N15/815 ,  C12N15/68 ,  Y10S435/938

公开(公告)号：EP0242359A4

公开(公告)日：1989-02-23

申请号：EP86900017

申请日：1985-12-04

申请人： BIOTECH AUSTRALIA PTY LTD

发明人： CLARK SUSAN JOY ,  CAHILL ANN DENISE

IPC分类号： C12N15/00 ,  A61K20060101 ,  A61K39/00 ,  A61K39/108 ,  C07G99/00 ,  C07H20060101 ,  C07H21/00 ,  C07K20060101 ,  C07K14/005 ,  C07K14/195 ,  C07K14/245 ,  C07K14/41 ,  C07K16/00 ,  C12N20060101 ,  C12N1/00 ,  C12N1/20 ,  C12N15/09 ,  C12N15/68 ,  C12P19/34 ,  C12P21/00 ,  C12R1/19 ,  C07G17/00 ,  C07K15/00 ,  C07K15/12

CPC分类号： C12N15/68 ,  A61K39/00 ,  C07K14/245

公开(公告)号：EP0281104A1

公开(公告)日：1988-09-07

申请号：EP88103172.8

申请日：1988-03-02

申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

发明人： Shimazu, Mitsunobu ,  Terasawa, Masato ,  Yukawa, Hideaki

IPC分类号： C12N15/60 ,  C12N15/68 ,  C12N1/20 ,  C12P13/22

CPC分类号： C12N15/68 ,  C12N9/88 ,  C12N15/74 ,  C12P13/227

摘要： A plasmid consisting essentially of

(a) a DNA fragment containing tryptophanase structural genes,
(b) a DNA fragment containing a tryptophan promoter and operator which control the expression of the tryptophanase structural genes,
(c) a DNA fragment containing genes governing the automonous replication system of a Col E1-type plasmid, and
(d) a DNA fragment containing genes derived from F-plasmid and governing its partition system.
Microorganisms transformed with the said plasmid were useful for the production of L-tryptophan having excellent producibility of tryptophanase.

摘要翻译： 一种基本上由（a）含有色氨酸酶结构基因的DNA片段组成的质粒，（b）含有色氨酸启动子的DNA片段和控制色氨酸酶结构基因表达的操纵子，（c）含有控制自动复制基因的DNA片段 Col E1型质粒的系统，（d）含有来自F-质粒的基因的DNA片段，并控制其分配系统。 用所述质粒转化的微生物可用于生产具有优异色氨酸酶生产能力的L-色氨酸。