公开(公告)号：EP0857217A2

公开(公告)日：1998-08-12

申请号：EP96936537.0

申请日：1996-10-16

申请人： DANA-FARBER CANCER INSTITUTE

发明人： MARASCO, Wayne A. ,  RICHARDSON, Jennifer ,  PAROLIN, Maria Cristina ,  SODROSKI, Joseph G.

IPC分类号： C07K14 ,  C12N15

CPC分类号： C07K14/005 ,  C12N15/86 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention is related to vectors and methods for increasing the expression of a desired gene product. Preferably this invention is used with genes expressing proteins that are not well tolerated by mammalian cells or where high levels of expression are necessary. In certain preferred embodiments it can be used as part of a multi-tiered expression system and with methods of intracellularly targeting a molecule.

公开(公告)号：EP0853121A3

公开(公告)日：1998-08-05

申请号：EP98105190.7

申请日：1989-12-20

申请人： Genentech, Inc.

发明人： Shak, Steven

IPC分类号： C12N9/22 ,  A61K38/46 ,  C12N15/55 ,  C12N15/83

CPC分类号： C12Q1/6876 ,  A61K38/00 ,  C12N9/22 ,  C12N11/08 ,  C12N15/71 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/44

摘要： DNA isolates coding for human DNase and methods of obtaining such DNA are provided, together with expression systems for recombinant production of human DNase useful in therapeutic or diagnostic compositions.

摘要翻译： 提供了编码人DNA酶的DNA分离物和获得这种DNA的方法，以及用于重组生产用于治疗或诊断组合物的人DNase的表达系统。

公开(公告)号：EP0831925A1

公开(公告)日：1998-04-01

申请号：EP96917935.0

申请日：1996-06-03

申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH ,  THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE

发明人： DRANOFF, Glenn ,  MULLIGAN, Richard C. ,  PARDOLL, Drew

IPC分类号： C12N15 ,  A61K38 ,  A61K39 ,  A61K48 ,  A61P35 ,  A61P37 ,  A61P43 ,  C07K14 ,  C12N5

CPC分类号： A61K39/21 ,  A61K38/00 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/5152 ,  A61K2039/5156 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/55522 ,  A61K2039/57 ,  C07K14/52 ,  C07K14/525 ,  C07K14/535 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/545 ,  C07K14/55 ,  C07K14/57 ,  C07K14/705 ,  C07K14/70507 ,  C07K14/70525 ,  C12N15/85 ,  C12N2740/13034 ,  C12N2799/027 ,  C12N2840/44

摘要： A method of altering the specific, systemic immune response of an individual to a target antigen by the co-administration of a cytokine, an adhesion or accessory molecule, and the target antigen. The target antigen may be a tumor cell, a tumor cell antigen, an infectious agent or other foreign antigen, or other antigens to which an enhanced systemic immune response is desirable. Alternatively, the antigen may be a non-foreign antigen when a suppression of a systemic immune response is desired. The resulting systemic immune response is specific for the target antigen.

公开(公告)号：EP0815232A1

公开(公告)日：1998-01-07

申请号：EP96910432.0

申请日：1996-03-12

申请人： TRANSKARYOTIC THERAPIES, INC.

发明人： TRECO, Douglas, A. ,  HEARTLEIN, Michael, W. ,  HAUGE, Brian, M. ,  SELDEN, Richard, F.

IPC分类号： C12N15 ,  A61K35 ,  A61K38 ,  A61K48 ,  A61P7 ,  A61P25 ,  A61P27 ,  A61P37 ,  C07K14 ,  C12N5 ,  C12N9

CPC分类号： A61K48/00 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/505 ,  C07K14/524 ,  C07K14/565 ,  C07K14/605 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/16 ,  C12N15/67 ,  C12N15/85 ,  C12N15/907 ,  C12N2510/02 ,  C12N2840/44

摘要： The invention relates to novel human DNA sequences, targeting constructs, and methods for producing novel genes encoding thrombopoietin, DNase I, and β-interferon by homologous recombination. The targeting constructs comprise at least: (a) a targeting sequence; (b) a regulatory sequence; (c) an exon; and (d) a splice-donor site. The targeting constructs, which can undergo homologous recombination with endogenous cellular sequences to generate a novel gene, are introduced into cells to produce homologously recombinant cells. The homologously recombinant cells are then maintained under conditions which will permit transcription of the novel gene and translation of the mRNA produced, resulting in production of either thrombopoietin, DNase I, or β-interferon. The invention further relates to methods of producing pharmaceutically useful preparations containing thrombopoietin, DNase I, or β-interferon from homologously recombinant cells and methods of gene therapy comprising administering homologously recombinant cells producing thrombopoietin, DNase I, or β-interferon to a patient for therapeutic purposes.

公开(公告)号：EP0814159A2

公开(公告)日：1997-12-29

申请号：EP97201755

申请日：1991-08-28

申请人： GENPHARM INT

发明人： LONBERG NILS ,  KAY ROBERT

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K39/395 ,  C07K14/725 ,  C07K16/00 ,  C07K16/18 ,  C07K16/28 ,  C07K16/30 ,  C07K16/42 ,  C07K16/44 ,  C07K16/46 ,  C12N5/10 ,  C12N5/16 ,  C12N15/00 ,  C12N15/09 ,  C12N15/13 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12P21/08 ,  C12N5/06

CPC分类号： C12N15/907 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A61K38/00 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4266 ,  C07K16/4291 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C12N5/163 ,  C12N15/00 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/36 ,  C12N2830/60 ,  C12N2840/44

摘要： A transgenic mouse having a genome comprising a human immunoglobulin heavy chain minilocus transgene, the transgene being capable of rearrangement and switching in the mouse, whereby two or more rearranged human heavy chain isotypes are produced.

摘要翻译： 一种具有包含人免疫球蛋白重链微型转基因的基因组的转基因小鼠，所述转基因能够在小鼠中重排和切换，由此产生两个或更多个重排的人重链同种型。

公开(公告)号：EP0787200A2

公开(公告)日：1997-08-06

申请号：EP95942839.0

申请日：1995-10-27

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： WILSON, James, M. ,  FISHER, Krishna, J. ,  CHEN, Shu-Jen ,  WEITZMAN, Matthew

IPC分类号： C12N15 ,  A61K35 ,  A61K48 ,  C12N7

CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2810/6018 ,  C12N2840/44

摘要： A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.

公开(公告)号：EP0784690A1

公开(公告)日：1997-07-23

申请号：EP95923019.0

申请日：1995-06-07

申请人： GENVEC, INC.

发明人： KOVESDI, Imre ,  BROUGH, Douglas, E. ,  McVEY, Duncan, L. ,  BRUDER, Joseph, T. ,  LIZONOVA, Alena

IPC分类号： C12N15 ,  A61K35 ,  A61K48 ,  A61P43 ,  C07K14 ,  C12N5 ,  C12Q1 ,  A61K38 ,  C12R1

CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/4712 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2810/60 ,  C12N2810/6081 ,  C12N2830/002 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44 ,  Y10S977/799

摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.

公开(公告)号：EP0759082A1

公开(公告)日：1997-02-26

申请号：EP95919831.0

申请日：1995-05-11

申请人： TRANSKARYOTIC THERAPIES, INC.

发明人： TRECO, Douglas A. ,  HEARTLEIN, Michael W. ,  SELDEN, Richard F.

IPC分类号： C12N15 ,  A61K31 ,  A61K38 ,  A61K39 ,  A61K48 ,  A61P43 ,  C07H21 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12P21 ,  C12R1

CPC分类号： A61K48/00 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/505 ,  C07K14/605 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/67 ,  C12N15/85 ,  C12N15/907 ,  C12N2510/02 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/702 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/44

摘要： The invention relates to constructs comprising: a) a targeting sequence; b) a regulatory sequence; c) an exon; and d) an unpaired splice-donor site. The invention further relates to a method of producing protein in vitro or in vivo comprising the homologous recombination of a construct as described above within a cell. The homologously recombinant cell is then maintained under conditions which will permit transcription and translation, resulting in protein expression. The present invention further relates to homologously recombinant cells, including primary, secondary, or immortalized vertebrate cells, methods of making the cells, methods of homologous recombination to produce fusion genes, methods of altering gene expression in the cells, and methods of making a protein in a cell employing the constructs of the invention.

公开(公告)号：EP0747485A1

公开(公告)日：1996-12-11

申请号：EP95119039.6

申请日：1990-11-06

申请人： CELL GENESYS, INC.

发明人： Skoultchi, Arthur I.

IPC分类号： C12N15/85 ,  C12N5/10 ,  C07K14/71 ,  C12N15/90

CPC分类号： C12N15/81 ,  C07K14/505 ,  C07K14/535 ,  C07K14/59 ,  C12N15/85 ,  C12N15/86 ,  C12N15/902 ,  C12N2795/00011 ,  C12N2800/206 ,  C12N2840/20 ,  C12N2840/44

摘要： Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.

摘要翻译： 提供了用于在培养物中表达哺乳动物基因的方法和组合物。 通过与靶基因并置引入同源重组引入可扩增基因，将可扩增基因和靶基因的组合转移到方便的宿主，并通过可扩增基因扩增靶基因。 然后可以使得到的表达宿主在靶基因增强表达的培养物中生长。

公开(公告)号：EP0695351A1

公开(公告)日：1996-02-07

申请号：EP94913174.0

申请日：1994-04-21

申请人： UNIVERSITY OF EDINBURGH

发明人： SMITH, Austin, Gerard AFRC Centre Genome Research ,  MOUNTFORD, Peter, Scott Stem Cell Sciences

IPC分类号： A01K67 ,  C07K14 ,  C12N5 ,  C12N9 ,  C12N15 ,  C12Q1 ,  A61K38

CPC分类号： C12N15/907 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/4702 ,  C07K14/52 ,  C07K14/70596 ,  C07K14/81 ,  C12N5/0623 ,  C12N9/12 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/873 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/44

摘要： Animal stem cells are obtained and maintained by culturing cells containing, in the genome, a selectable marker; differential expression of the selectable marker enables preferential survival and/or division of the desired stem cells compared to non-stem cells. The selectable marker can be an antibiotic resistance gene.