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65 条记录 (耗时 0.018 秒)

    MULTIPLEXED NUCLEIC ACID ANALYSIS BY FRAGMENTATION OF DOUBLE-STRANDED DNA
    21.
    发明公开
    MULTIPLEXED NUCLEIC ACID ANALYSIS BY FRAGMENTATION OF DOUBLE-STRANDED DNA 有权
    MULTIPLEX-NUKLEINSÄUREANALYSEMITTELS FRAGMENTIERUNGDOPPELSTRÄNGIGERDNA

    公开(公告)号：EP1694859A4

    公开(公告)日：2007-08-08

    申请号：EP04796412

    申请日：2004-10-26

    申请人： BIOARRAY SOLUTIONS LTD

    发明人： YANG JIACHENG

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2565/519 ,  C12Q2523/107

    摘要： A method of fragmentation of double stranded DNA is disclosed for use in nucleic acid analysis, notably in the multiplexed analysis of polymorphisms and mutations. The method produces a multiplicity of labeled sense and anti-sense fragments which are not complementary, and thus do not significantly re-anneal under conditions suitable for hybridization analysis (or capture-mediated elongation analysis) of the polymorphisms and/or mutations. The fragments display a desired or predicted length distribution. Cleavage sites can be selected such that the fragments are short, yet long enough to allow discrimination among fragments in an assay, and as a matter of statistical probability, such that the majority of fragments contain at least one labeled nucleotide to facilitate detection.

    摘要翻译： 公开了用于核酸分析的双链DNA片段化方法，特别是在多态性和突变的多重分析中。 该方法产生多个标记的有义和反义片段，它们不互补，因此在适合于多态性和/或突变的杂交分析（或捕获介导的延伸分析）的条件下不显着再退火。 该片段显示期望的或预测的长度分布。 可以选择切割位点，使得片段短而足够长以允许在测定中片段之间的区分，并且作为统计概率的问题，使得大多数片段含有至少一个标记的核苷酸以便于检测。

    Molecular constructs and methods of use for detection of biochemical reactions
    25.
    发明公开
    Molecular constructs and methods of use for detection of biochemical reactions 有权
    Methode zur Anwendung Molekulare Konstrukte zur Detektion biochemischer Reaktionen

    公开(公告)号：EP1394270A2

    公开(公告)日：2004-03-03

    申请号：EP03018640.7

    申请日：2003-08-20

    申请人： Bioarray Solutions Ltd

    发明人： Yang, Jiacheng

    IPC分类号： C12Q1/68 ,  G01N33/543 ,  C12Q1/25

    CPC分类号： C12Q1/6837 ,  C07K14/003 ,  C07K2319/50 ,  C12N2310/3181 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/485 ,  C12Q1/6827 ,  G01N33/54366 ,  G01N33/6893 ,  G01N33/6896 ,  C12Q2525/107 ,  C12Q2525/161 ,  C12Q2537/137 ,  C12Q2565/518 ,  C12Q2563/119

    摘要： The present invention relates to molecular constructs and methods of their use in detecting biochemical reactions. In particular, the invention relates to a molecular construct having a capture portion and a substrate portion, where the capture portion isolates the construct from a sample medium, and the substrate portion enables the construct to be acted upon and undergo a physical change which can be detected and measured. These molecular constructs may be used in diagnostic assays, genetic screening for potential risks of certain diseases in individuals, and drug discovery and toxicogenomics, using high throughput screening of compounds.

    摘要翻译： 本发明涉及其用于检测生物化学反应的分子构建体及其方法。 特别地，本发明涉及具有捕获部分和底物部分的分子构建体，其中捕获部分将构建体与样品介质隔离，并且衬底部分使构建体能够作用于并经历物理变化，其可以是 检测和测量。 这些分子构建体可用于诊断测定，遗传筛选个体中某些疾病的潜在风险，以及药物发现和毒物基因组学，使用化合物的高通量筛选。