公开(公告)号：EP2617829A4

公开(公告)日：2014-05-07

申请号：EP11824375

申请日：2011-09-13

申请人： FUNDACAO OSWALDO CRUZ ,  ALVOS CONSULTORIA DESENVOLVIMENTO E COM IZ

发明人： TENDLER MIRIAM ,  RAMOS CELSO RAUL ROMERO RAMOS ,  SIMPSON ANDREW J G

IPC分类号： C12N15/12 ,  A61K38/17 ,  A61K39/17 ,  C12N15/63 ,  C12N15/79

CPC分类号： C07K1/36 ,  A61K38/00 ,  A61K39/0003 ,  C07K14/395 ,  C07K14/43559 ,  C12N15/815

摘要： The present invention is related to the field of recombinant protein production using a synthetic gene associated with high protein expression in Pichia pastoris. More specifically, this invention describes the production of Sm14 Schistosoma mansoni recombinant protein. A synthetic gene was created to promote high Sm14 expression, and with this gene we obtained and genetically manipulated a Pichia pastoris strain for effectively producing a vaccine. We have also improved that protein's production and purification processes from P. pastoris cells; the industrial production of such processes may be scheduled.

公开(公告)号：EP2572576A1

公开(公告)日：2013-03-27

申请号：EP11782792.3

申请日：2011-05-18

申请人： Fundacao Oswaldo Cruz - Fiocruz

发明人： BAPTISTA, Darcilio Fernandes ,  MUGNAI, Riccardo ,  DE OLIVEIRA, Renata Bley

IPC分类号： A01M23/02

CPC分类号： G01N1/10 ,  A01K61/90 ,  G01N1/12 ,  G01N1/18 ,  G01N33/186 ,  G01N2001/1012

摘要： The present invention refers to the subsampler and to a subsampling method that allows for the execution of environmental monitoring without the use of large sample volumes, thus ensuring specimen wealth and expedited analyses.

公开(公告)号：EP2102344A4

公开(公告)日：2012-02-22

申请号：EP06804584

申请日：2006-10-20

申请人： FUNDACAO OSWALDO CRUZ

发明人： GOES ANA CAROLINA MAGALHAES ,  LEITE JOSE PAULO GAGLIARDI ,  DE MORAES SOUZA MARCIA TEREYINHA BARONI ,  ARAUJO IRENE TRIGUEIROS ,  D HALLUIN JEAN CLAUDE ,  DA SILVA JR JOSE GODINHO

IPC分类号： C12N15/40 ,  C07K14/08 ,  C07K14/14 ,  C12N15/46 ,  G01N33/569

CPC分类号： C07K14/005 ,  C12N2720/12322 ,  C12N2770/12022 ,  G01N33/56983 ,  G01N2333/14

公开(公告)号：EP2024497A4

公开(公告)日：2010-03-17

申请号：EP06828120

申请日：2006-12-19

申请人： FUNDACAO OSWALDO CRUZ

发明人： MEDEIROS MARCO ALBERTO ,  DOS REIS MITEMAYER GALVAO ,  DA SILVA EVERTON FAGONDE ,  MCBRIDE ALAN JOHN ALEXANDER ,  CRODA JULIO HENRIQUE ROSA ,  RAMOS JOAO GABRIEL ROSA ,  DELLAGOSTIN ODIR ANTONIO ,  MCBRIDE FLAVIA WEYKAMP CRUZ ,  KO ALBERT ICKSANG

IPC分类号： C12N15/09 ,  A61K39/02 ,  C07K14/20 ,  G01N33/53

CPC分类号： C07K14/20 ,  G01N33/56911 ,  G01N2333/20 ,  G01N2469/20

摘要： The present invention refers to isolated DNA (deoxyribonucleic acid) molecules that encode for LigA and LigB proteins. The invention further refers to the use of fragments of these proteins in the diagnosis, and formulation of vaccines for prevention of infections caused by Leptospira in humans and animals.

摘要翻译： 本发明涉及编码LigA和LigB蛋白的分离的DNA（脱氧核糖核酸）分子。 本发明还涉及这些蛋白质的片段在诊断和制备用于预防人和动物中由钩端螺旋体引起的感染的疫苗中的用途。

公开(公告)号：EP1819352A4

公开(公告)日：2010-03-03

申请号：EP05764528

申请日：2005-07-21

申请人： FIOCRUZ FUNDACAO OSWALDO CRUZ

发明人： HENRIQUES MARIA DAS GRACAS MUL ,  MONTEIRO CARMEM PENIDO ,  SIANI ANTONIO CARLOS ,  GUILHERMINO JISLAINE DE FATIMA ,  RAMOS MONICA FREIMAN DE SOUZA ,  SAMPAIO ANDRE LUIZ FRANCO ,  ROSAS ELAINE CRUZ ,  DE LIMA LUCILENE AZEVEDO ,  PENNAFORTE RENATO JABOUR

IPC分类号： A61K36/18 ,  A61P37/00

CPC分类号： A61K9/0014 ,  A61K9/0095 ,  A61K36/58 ,  A61K47/08 ,  A61K47/44 ,  A61K2300/00

公开(公告)号：EP2004832A2

公开(公告)日：2008-12-24

申请号：EP06804599.6

申请日：2006-11-01

申请人： Fundacao Oswaldo Cruz - Fiocruz

发明人： GAZZINELLI, Ricardo Tostes ,  BRUNA-ROMERO, Oscar ,  CAETANO, Bráulia Costa

IPC分类号： C12N15/861 ,  C12N15/30

CPC分类号： A61K39/002 ,  A61K2039/5254 ,  C07K14/45 ,  C12N2799/022

摘要： This invention refers to the construction of recombinant adenovirus with genes that codify for T.gondii SAG1, SAG2 and SAG3, through a homologue recombination technique between two vectors. A first vector that serves as transference vector of genes and a second vector that bears the adenoviral receptor genome of these genes. The invention is also related to the use of recombinant adenovirus in a vaccine composition to obtain the immunization against infections caused by T.gondii parasite.

公开(公告)号：EP1514104B1

公开(公告)日：2007-10-03

申请号：EP02807433.4

申请日：2002-05-20

申请人： FUNDACAO OSWALDO CRUZ (FIOCRUZ)

发明人： KO, Albert, I., C. de Pesquisas Gonçalo Moniz/Fio. ,  GALVAO REIS, Mitermayer, Centro de Pesquisas ,  ROSA CRODA, Julio, H., Centro de Pesquisas ,  SIQUEIRA, Isadora, C., Centro de Pesquisas Goncalo ,  HAAKE, David, A. ,  MATSUNAGA, James ,  RILEY, Lee, W. ,  BAROCCHI, Michele ,  YOUNG, Tracy, A.

IPC分类号： G01N33/53 ,  C07K17/00

CPC分类号： C07K14/20 ,  A61K38/00 ,  A61K39/00 ,  C07K16/1207 ,  G01N33/56911 ,  G01N33/6854 ,  G01N2333/20 ,  Y02A50/48 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

公开(公告)号：EP1318824B1

公开(公告)日：2005-03-16

申请号：EP01959996.8

申请日：2001-08-29

申请人： FUNDACAO OSWALDO CRUZ (FIOCRUZ)

发明人： FERREIRA RODRIGUES HEERKLOTZ, Kátia ,  HEERKLOTZ, Jörg, Arno ,  WERNER, Christa

IPC分类号： A61K35/70 ,  C12P17/04

CPC分类号： A61K36/062 ,  C07D317/34 ,  C12P17/04 ,  Y02A50/473 ,  Y10S435/911

摘要： The present invention refers to extracts from the fungus Guignardia sp., and also a new isolate compound from the aforementioned extract, both having antimicrobial activities, particularly antibacterial and antifungal activities, and their use in pharmaceutical compositions. The new compound, isolated from the extract of the fungus Guignardia sp., presents a dioxolanone ring, having the following formula:

公开(公告)号：EP1495047A2

公开(公告)日：2005-01-12

申请号：EP03709469.5

申请日：2003-04-01

申请人： Fundacao Oswaldo Cruz - Fiocruz

发明人： TENDLER, Miriam ,  GARRAT, Richard, Charles ,  KATZ, Naftale ,  SIMPSON, Andrew, John, George ,  DE BARRIENTOS, Frank, Jefferson, Alarcon ,  VILAR, Mônica, Magno ,  ALMEIDA, Marilia, Sirianni, dos Santos

IPC分类号： C07K14/435 ,  A61K38/17 ,  G01N33/53

CPC分类号： A61K39/0003 ,  A61K39/00 ,  C07K14/4354 ,  C07K14/43559 ,  C07K2319/00 ,  G01N33/6893 ,  Y02A50/423

摘要： The present invention relates to peptide fragments which have one or more shared and/or similar amino acid sequences to amino acid sequences of specific portions of the 14 kDa protein of S. mansoni (Sm14) or related FABPs (Fatty Acid Binding Proteins), the said peptide fragments functioning as continuous or discontinuous epitopic regions of the molecule or mimicking its biological activity. More particularly, the present invention relates to a method for constructing active peptide fragments, peptide fragments, immunogenic composition and diagnostic kit using said peptide fragments.

公开(公告)号：EP1989316A4

公开(公告)日：2011-01-05

申请号：EP06804595

申请日：2006-10-31

申请人： FUNDACAO OSWALDO CRUZ

发明人： BONALDO MYRNA CRISTINA ,  GALLER RICARDO

IPC分类号： C12N15/86 ,  A61K35/76 ,  A61K39/12 ,  A61K48/00 ,  C12N7/00 ,  C12N15/63

CPC分类号： A61K39/12 ,  A61K2039/525 ,  A61K2039/5256 ,  A61K2039/575 ,  C07K14/005 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/40 ,  C12N7/00 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24143 ,  C12N2770/24171

摘要： The purpose of the present invention is the production of recombinant virus through the cloning and expression of sequences of coding nucleotides of the whole or part of heterolog proteins, through the following method: (a) modification of the heterolog nucleotides sequences in such way they when cloned and expressed in the vector virus, they present in the 5′ region, nucleotides present in the 5′ edge of the gene NS1 of this vector virus or of other virus or equivalent functional sequences, and in its 3′ region, the correspondent genome region in the whole or part of the spheres of the steam and anchor of the protein E of this vector virus or equivalent functional sequences, and not compromising the structure and the replication of the mention vector virus; (b) insertion of the modified heterolog sequences in (a) in the intergene region at the structural protein E level and of on structural NS1 vector virus; (c) obtention of the non pathogenic recombinant virus and owner of the immunologic properties, having the heterolog sequences integrated in the viral genome according to the insertion described in (b) and, like that, expressing the heterolog antigene in such way that it can induce an appropriate immune response. The present invention is also addressed to vaccine compositions to immune against the Flavivirus and/or other pathogens.

摘要翻译： 本发明的目的是通过编码全部或异源蛋白质的一部分的核苷酸，通过以下方法的序列的克隆和表达产生重组病毒组成：（a）在寻求方式他们当异源的核苷酸序列的修饰 克隆并在所述载体的病毒，在存在于5“区的核苷酸存在于5”基因此载体病毒的病毒或其它病毒或等价的功能序列的NS1的边缘过度表达，并在其3“区域，对应的基因组区 在整个或蒸汽和该载体病毒或等效的功能序列，并且不包含所述结构和所述病毒载体提及的复制的蛋白E的锚的球体的一部分; （B）在修改的异源序列插入的（a）中在结构蛋白E水平和constructural NS1病毒载体的基因间区域; （C）的非致病性的重组病毒和免疫学特性的所有者的获得方法，其具有集成在病毒基因组雅丁到在（b）和，这样，表达在寻求方式所述异源抗原做到了可以描述插入的异源序列 诱导到适当的免疫应答。 因此，本发明致力于疫苗组合物对抗黄病毒和/或其它免疫patogens。