摘要:
A DNA construct consisting of: (1) a screening marker gene; (2) a proliferation inhibitory sequence which can be induced with galactose; (3) a pair of FRT sequences located in such a direction that (1) and (2) are sandwiched between them; and (4) a DNA which can be recombined with yeast chromosomal DNAs provided in both sides of (3); characterized in that the FRT sequences contain the following sequence: 5'- GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC -3'(SEQ ID NO:1) inverted repeat spacer inverted repeat sequence (1) sequence sequence (2) or a sequence substantially identical therewith and, in each of the pairing FRT sequences, one to six bases have been deleted from the distal side of the spacer sequence in the inverted repeat sequence in the distal side of the screening marker gene and the proliferation inhibitory sequence sandwiched between them; a method of transforming a yeast belonging to the genus Saccharomyces by using the same; a yeast belonging to the genus Saccharomyces transformed thereby; and a process for producing beer characterized by using this yeast belonging to the genus Saccharomyces .
摘要:
A recombinant yeast belonging to the genus Saccharomyces having a promoter of a consitutively expressed gene and a structural gene coding for O-acetylhomoserine sulfhydrylase (MET25) under the control by said promoter so that the recombinant yeast produces an amount of hydrogen sulfide lower than the amount of hydrogen sulfide produced by the parental yeast from which the recombinant yeast is derived.
摘要:
The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要:
The present invention relates to a mutant acetohydroxy-acid reductoisomerase gene (mutant ILV5 gene) and use thereof, in particular, a brewery yeast for producing alcoholic beverages with superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. According to the method for producing alcoholic beverages of the present invention, because of reduction of the production of vicinal diketones, especially diacetyl, which are responsible for off -flavors in products, alcoholic beverages with superior flavor can be produced.
摘要:
The present invention relates to a esterase gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent aroma and flavor, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing ester, which contribute to aroma and flavor of products, was controlled by regulating expression level of IAH1 gene encoding brewery yeast esterase Iah1p, particularly nonScIAH1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要:
The present invention relates to modified industrial yeast strains that show reduced hydrogen sulfide production. In one embodiment the invention provides an industrial yeast strain comprising a modification in a MET5 gene and/or a MET10 gene which results in reduced hydrogen sulfide production when compared to the corresponding industrial yeast strain without the modification. The present invention also relates to methods of manufacturing these modified industrial yeast strains and their use in the production of fermented products.
摘要:
The present invention relates to a esterase gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent aroma and flavor, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing ester, which contribute to aroma and flavor of products, was controlled by regulating expression level of IAH1 gene encoding brewery yeast esterase Iah1p, particularly nonScIAH1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要:
The present invention describes The present invention relates to yeast strains with improved carbohydrate fermentation capacity, in particular to strains transformed with a gene encoding an improved hexose transporter gene, more in particular a mutated HXT3 gene.