公开(公告)号：EP0817837A1

公开(公告)日：1998-01-14

申请号：EP96908123.0

申请日：1996-03-21

申请人： KARO BIO AB

发明人： ÖHMAN, Lars ,  HÄGGSTRÖM, Lena, Royal Inst. Technology

IPC分类号： C12N5 ,  C12N9

CPC分类号： C12N5/0601 ,  C12N9/2471 ,  C12N2500/05 ,  C12N2500/32 ,  C12Y302/01023

摘要： A culture medium for insect cells, particularly for culturing insect cells for the expression of proteins using a baculovirus expression system, characterized in that the medium does not contain glutamine.

公开(公告)号：EP0555454A1

公开(公告)日：1993-08-18

申请号：EP92918738.0

申请日：1992-08-28

申请人： CELLTECH LIMITED

发明人： BORASTON, Robert Charles

IPC分类号： C12N5 ,  C12P21

CPC分类号： C12N5/0018 ,  C12N2500/05 ,  C12N2500/12

摘要： Des cellules animales adhérentes sont développées en suspension dans un milieu nutritif dans lequel le rapport molaire de la totalité des ions organiques et de la totalité des acides aminés est pratiquement réduit si on le compare au rapport correspondant d'un milieu nutritif connu, et est maintenu à un niveau où se produit peu ou aucune agrégation cellulaire.

公开(公告)号：EP0230430A1

公开(公告)日：1987-08-05

申请号：EP86903165.0

申请日：1986-06-12

申请人： LUMINIS PTY. LIMITED ,  QUINN, Patrick James

发明人： QULNN, Patrick, James

IPC分类号： C12N5

CPC分类号： C12N5/0609 ,  C12N2500/05 ,  C12N2500/34

摘要： Milieu de culture pour la fécondation in vitro d'ovocytes humains comprenant les éléments suivants: NaCl (96,5-106,7 mM) KCl (4,46 - 4,92 mM) MgSO4.7H2O (0,18 - 0,22 mM) KH2PO4 (0,35 - 0,39 mM) CaCl2.2H2O (1,94 - 2,14 mM) NaHCO3 (23,7 - 26,3 mM) Glucose (2,64 - 2,92 mM) Pyruvate de Sodium (0,31 - 0,35 mM) Lactate de sodium (20,3 - 22,5 mM) Pénicilline (95 - 105 unités/ml) Rouge de phénol (5 - 15 microgrammes/ml). Les milieux de culture dans lesquels le rapport des ions de sodium sur les ions de potassium est de l'ordre de 28 à 32 sont préférés.

公开(公告)号：EP2882846B1

公开(公告)日：2018-09-05

申请号：EP13799830.8

申请日：2013-06-05

申请人： The Regents of the University of California

发明人： CLEGG, Dennis ,  BUCHHOLZ, David ,  CROZE, Roxanne ,  PENNINGTON, Britney ,  COFFEY, Peter ,  LEACH, Lyndsay

IPC分类号： C12N5/02 ,  C12N5/079 ,  C12N5/0793

CPC分类号： C12N5/0621 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/35 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/02

摘要： The invention relates to the field of cell culture, specifically, the derivation of retinal pigmented epithelial cells from pluripotent cells. The invention comprises the use of various cell culture medium supplements, medium formulations, and methods of using such medium supplements and formulations, in order to effect the rapid differentiation of pluripotent cells into retinal pigmented epithelial cells with very high yields. The invention further includes cell culture media formulations for the efficient maintenance, propagation, and maturation of cultured retinal pigmented epithelial cells.

公开(公告)号：EP2885969B1

公开(公告)日：2018-06-27

申请号：EP14198971.5

申请日：2014-12-18

申请人： FertiPro N.V.

发明人： Comhaire, Sven ,  Comhaire, Björn ,  Eertmans, Frank ,  Bogaert, Veerle

IPC分类号： A01N1/02

CPC分类号： C12N5/0018 ,  A01N1/0221 ,  C12N5/0656 ,  C12N5/0668 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2500/90

摘要： Provided herein are methods for the cryopreservation of stem cells and/or progenitor cells, comprising the steps of (a) contacting stem cells and/or progenitor cells with a cryopreservation medium, said cryopreservation medium comprising between 4 v/v% and 25 v/v% of propylene glycol and between 1.0 w% and 10 w% of a sugar; and (b) slow-freezing said cells, thereby obtaining a frozen composition comprising said cells and said cryopreservation medium.

公开(公告)号：EP3243903A1

公开(公告)日：2017-11-15

申请号：EP17172768.8

申请日：2013-02-11

申请人： Washington State University

发明人： OATLEY, Jon Michael ,  OATLEY, Melissa Joan

IPC分类号： C12N5/076 ,  A61K35/52 ,  C12N15/873 ,  C12N5/071

CPC分类号： C12N5/061 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  A61B17/425 ,  A61D19/00 ,  A61K35/52 ,  C12M21/00 ,  C12N5/0602 ,  C12N5/0603 ,  C12N5/0656 ,  C12N5/0683 ,  C12N15/873 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/246 ,  C12N2510/04 ,  C12N2533/90

摘要： The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

公开(公告)号：EP2889373A1

公开(公告)日：2015-07-01

申请号：EP14196176.3

申请日：2014-12-04

申请人： Yeditepe Universitesi

发明人： Sahin, Fikrettin ,  Tasli, Pakize Neslihan

IPC分类号： C12N5/077

CPC分类号： C12N5/0654 ,  C12N2500/05 ,  C12N2500/14 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/50 ,  C12N2501/30 ,  C12N2501/39 ,  C12N2506/1361

摘要： The present invention relates to a dental differentiation product which obtains tooth cell from adult stem cells. Tooth cells are effectively obtained with adult stem cells that are used in tissue engineering. When stems cells are differentiating, they lose their own properties and acquire new properties. These properties are both at morphological level and gene expression level. The product of the present invention comprises C 13 H 16 N 2 O) (melatonin), Ca(OH) 2 (calcium hydroxide), C 22 H 29 FOs (dexamethasone), C 3 H 7 Na 2 O 6 P ·5H 2 O (disodium beta-glycerophosphate hydrate), NaF (sodium fluoride) and Pluronic chemicals.

摘要翻译： 本发明涉及从成体干细胞获得牙细胞的牙齿分化产物。 用组织工程中的成体干细胞有效地获得牙细胞。 当干细胞分化时，它们失去自己的属性并获得新的特性。 这些性质都处于形态学水平和基因表达水平。 本发明的产品包括C 13 H 16 N 2 O）（褪黑激素），Ca（OH）2（氢氧化钙），C 22 H 29 FO（地塞米松），C 3 H 7 Na 2 O 6 P·5H 2 O（磷酸二氢钠水合物二钠），NaF（氟化钠）和Pluronic化学品。

公开(公告)号：EP2886644A1

公开(公告)日：2015-06-24

申请号：EP13306813.0

申请日：2013-12-20

申请人： Kallistem ,  UNIVERSITE CLAUDE BERNARD - LYON 1 ,  Centre National de la Recherche Scientifique (CNRS) ,  Institut National de la Recherche Agronomique (INRA) ,  ECOLE NORMALE SUPERIEURE DE LYON

发明人： Perrard, Marie-Hélène ,  Durand, Philippe ,  David, Laurent

IPC分类号： C12N5/076

CPC分类号： C12N5/061 ,  C12N5/0609 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/31 ,  C12N2501/385 ,  C12N2501/392 ,  C12N2533/72

摘要： The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.

摘要翻译： 本发明涉及一种用于从雄性生发组织进行体外精子发生的方法，其包括在生物反应器中包含生物反应器的包含生殖细胞的睾丸组织的成熟，所述生物反应器由生物材料制成并且包括至少一个空腔，其中放置生发组织，并且回收细长的精子细胞 和/或精子。

公开(公告)号：EP2885969A1

公开(公告)日：2015-06-24

申请号：EP14198971.5

申请日：2014-12-18

申请人： FertiPro N.V.

发明人： Comhaire, Sven ,  Comhaire, Björn ,  Eertmans, Frank ,  Bogaert, Veerle

IPC分类号： A01N1/02

CPC分类号： C12N5/0018 ,  A01N1/0221 ,  C12N5/0656 ,  C12N5/0668 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2500/90

摘要： Provided herein are methods for the cryopreservation of stem cells and/or progenitor cells, comprising the steps of (a) contacting stem cells and/or progenitor cells with a cryopreservation medium, said cryopreservation medium comprising between 4 v/v% and 25 v/v% of propylene glycol and between 1.0 w% and 10 w% of a sugar; and (b) slow-freezing said cells, thereby obtaining a frozen composition comprising said cells and said cryopreservation medium.

摘要翻译： 本文提供用于冷冻保存干细胞和/或祖细胞的方法，包括以下步骤：（a）将干细胞和/或祖细胞与冷冻保存介质接触，所述冷冻保存介质包含4v / v％至25v / v％的丙二醇和1.0w％-10w％的糖; 和（b）使所述细胞慢速冷冻，由此获得包含所述细胞和所述冷冻保存介质的冷冻组合物。

公开(公告)号：EP2864473A1

公开(公告)日：2015-04-29

申请号：EP13733516.2

申请日：2013-06-24

申请人： Seraxis, Inc.

发明人： RUST, William, L.

IPC分类号： C12N5/071 ,  C12N5/074

CPC分类号： A61K35/39 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/60 ,  C12N2506/22 ,  C12N2506/45

摘要： Fresh human pancreas tissue can be used as a source of cells when to identify and select a non-stem cell population that is predisposed to be a source for surrogate pancreatic cells that can be used in treating insulin-dependent diabetes. The progenitors of these surrogate pancreatic cells have no reprogramming genes integrated into their genomes, differentiate to the pancreatic lineage pursuant to a protocol that employs only defined reagents, and are substantially unable to differentiate to the mesodermal lineage.