公开(公告)号：EP0789074B1

公开(公告)日：2003-12-17

申请号：EP97400037.4

申请日：1997-01-08

Applicant: L'OREAL

Inventor： Schmidt, Rainer ,  Schmitt, Daniel ,  Regnier, Marcelle ,  Staquet, Marie-Jeanne

IPC: C12N5/06 ,  A61L27/00

CPC classification number: A61L27/60 ,  A61L27/3813 ,  A61L27/3886 ,  C12N5/0698 ,  C12N2500/25 ,  C12N2500/40 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/805 ,  C12N2502/091 ,  C12N2502/094 ,  C12N2502/1121 ,  C12N2502/1171 ,  C12N2502/1323

公开(公告)号：EP0787180B1

公开(公告)日：2002-09-04

申请号：EP95935994.4

申请日：1995-10-20

Applicant: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  ECOLE NORMALE SUPERIEURE DE LYON

Inventor： SAMARUT, Jacques ,  PAIN, Bertrand

IPC: C12N5/06 ,  C12N5/10 ,  A01K67/027

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/2306 ,  C12N2501/2311 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/385 ,  C12N2502/1323 ,  C12N2510/00

Abstract: A culture medium for avian totipotential embryonic cells comprising an avian cell culture medium is disclosed. The culture medium is characterised in that it comprises elements complementary to said avian cell culture medium, the complementary elements being selected from the group which comprises cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free of active retinoic acid. A method for culturing avian totipotential embryonic cells, and the resulting products, are also disclosed.

公开(公告)号：EP1025204A4

公开(公告)日：2001-02-28

申请号：EP98956192

申请日：1998-10-23

Applicant: GERON CORP

Inventor： BODNAR ANDREA G ,  CHIU CHOY-PIK ,  GOLD JOSEPH D ,  INOKUMA MARGARET ,  MURAI JAMES T ,  WEST MICHAEL D

IPC: C12N15/09 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/00 ,  C12N5/06 ,  C12N5/10 ,  C12N15/00 ,  C12N15/01

CPC classification number: C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2510/00

Abstract: Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

公开(公告)号：EP0703978B1

公开(公告)日：1999-08-18

申请号：EP94920264.2

申请日：1994-06-17

Applicant: AMGEN INC.

Inventor： PONTING, Ian L.O.

IPC: C12N5/06 ,  C12N5/08

CPC classification number: C12N5/0663 ,  C12N5/0647 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/40 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/39 ,  C12N2501/58 ,  C12N2502/1394

Abstract: A serum-free or serum-depleted medium for the short- and long-term proliferation and development of cells, particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development effective amounts of: a standard culture medium such as Iscove's modified Dulbecco's medium; serum albumin; transferrin; a source of lipids and fatty acids; cholesterol; a reducing agent; pyruvate; a glucocorticoid (when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth factor, fibroblast growth factor, platelet derived growth factor, and insulin; and extracellular matrix materials, such as collagen, fibronectin, and laminin.

公开(公告)号：EP0925370A1

公开(公告)日：1999-06-30

申请号：EP97930001.0

申请日：1997-06-18

Applicant: RESEARCH DEVELOPMENT FOUNDATION

Inventor： CRAWFORD, J., Fred ,  BUCCI, Luke

IPC: C12Q1 ,  C12N1 ,  C12N5 ,  G01N33

CPC classification number: C12N5/0634 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/95 ,  G01N33/5047

Abstract: The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution containing the following ingredients: a carbohydrate selected from the group consisting of glucose and a compound biologically capable of producing glucose in the cells; a biologically usable form of pantothenic acid; choline or a biological usable form of a substance capable of producing choline in the cells; inorganic ions comprising chloride, phosphate, calcium, magnesium, potassium, sodium; and iron in a biologically utilizable form, cumene hydroperoxide, deionized water, and a mitogen in an amount effective to stimulate the lymphocytes being assayed; said buffered, serum-free solution having a pH from about 6.8 to 7.6, said cell culture medium characterized by being effective to determine nutritional deficiencies, inadequacies, and imbalances and to biochemically analyze antioxidant function of the lymphocytes. Also provided is a method of biochemically analyzing cellular antioxidant function in an individual comprising the steps of: inoculating the cell culture medium of the present invention with lymphocytes from said individual; incubating the inoculated cell culture medium; and comparing the response of the lymphocytes with an average response of lymphocytes from a control group of individuals.

公开(公告)号：EP0285474B1

公开(公告)日：1992-12-09

申请号：EP88400502.6

申请日：1988-03-03

Applicant: CENTRE INTERNATIONAL DE RECHERCHES DERMATOLOGIQUES GALDERMA - CIRD GALDERMA

Inventor： Bernard, Bruno ,  Lenoir, Marie-Cécile ,  Shroot, Braham Villa 35 ,  Darmon, Yves-Michel ,  Asselineau, Daniel

IPC: A61L27/00 ,  A61F2/10

CPC classification number: C12N5/0698 ,  A61F2/105 ,  A61F2310/00365 ,  A61K35/12 ,  A61L27/24 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/40 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/092 ,  C12N2502/094 ,  C12N2502/1323

公开(公告)号：EP0285471B1

公开(公告)日：1991-01-16

申请号：EP88400489.6

申请日：1988-03-02

Applicant: CENTRE INTERNATIONAL DE RECHERCHES DERMATOLOGIQUES GALDERMA - CIRD GALDERMA Groupement d'Intérêt Economique dit:

Inventor： Bernard, bruno ,  Lenoir, Marie-Cécile ,  Shroot, Braham ,  Darmon, Yves-Michel ,  Asselineau, Daniel

IPC: A61L27/00 ,  C12N5/00

CPC classification number: C12N5/0698 ,  A61F2/10 ,  A61F2/105 ,  A61F2310/00365 ,  A61K35/12 ,  A61L27/24 ,  A61L27/36 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/40 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/092 ,  C12N2502/094 ,  C12N2502/1323

公开(公告)号：EP0387295A4

公开(公告)日：1990-12-19

申请号：EP89900448

申请日：1988-10-31

Applicant: GROB, HOWARD S. ,  FRIEDMAN, FRANK

Inventor： GROB, HOWARD S. ,  FRIEDMAN, FRANK

IPC: A01K67/02 ,  C12N5/02 ,  C12N5/075 ,  C12N5/00

CPC classification number: C12N5/0609 ,  C12N2500/40 ,  C12N2501/31 ,  C12N2501/33 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2517/10

公开(公告)号：EP0076647A2

公开(公告)日：1983-04-13

申请号：EP82305187.5

申请日：1982-09-30

Applicant: HANA Biologics Incorporated

Inventor： Steimer, Kathelyn Sue

IPC: C12N5/00

CPC classification number: C07K16/18 ,  C12N5/0634 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/90

Abstract: A culture medium, useful for the serum-free culturing of a variety of cells originating from the immune system and for culturing cells with a serum supplement, is disclosed. The cells to be cultured include, in addition to hybridomas, other cell types such as a variety of malignant cells of the immune system and normal lymphocytes.

Abstract translation: 公开了一种可用于免疫培养来自免疫系统的各种细胞和用血清补充物培养细胞的培养基。 要培养的细胞除了杂交瘤之外还包括其它细胞类型，例如免疫系统的多种恶性细胞和正常淋巴细胞。

公开(公告)号：EP3331911A1

公开(公告)日：2018-06-13

申请号：EP16750431.5

申请日：2016-08-04

Applicant: Richter Gedeon Nyrt.

Inventor： PUTICS, Ákos ,  ZALAI, Dénes ,  NAGY, Gáspár ,  PÁRTA, László ,  SCHLEICHER, Áron

IPC: C07K16/28 ,  C12N5/10

CPC classification number: C07K16/2887 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/734 ,  C12N5/0018 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2510/02

Abstract: The present invention relates to a method for increasing the galactose content of a recombinant protein produced in mammalian cells, wherein during the cultivation of said cells the pH of the cell culture is changed and a composition comprising nucleosides, transition metal salts and/or sugars is fed.