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    PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS
    21.
    发明公开
    PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS 有权
    UNG ON ON ON EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN

    公开(公告)号：EP2776571A1

    公开(公告)日：2014-09-17

    申请号：EP12795948.4

    申请日：2012-11-09

    申请人： Amyris, Inc.

    发明人： GARDNER, Timothy Stevens ,  HAWKINS, Kristy Michelle ,  MEADOWS, Adam Leon ,  TSONG, Annie Ening ,  TSEGAYE, Yoseph

    IPC分类号： C12P23/00 ,  C12P5/00 ,  C12N15/52

    CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1288 ,  C12N15/52 ,  C12N15/81 ,  C12P7/42 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y102/0101 ,  C12Y207/08

    摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

    摘要翻译： 本文提供了在宿主细胞中异源产生乙酰辅酶A衍生的类异戊二烯的组合物和方法。 在一些实施方案中，宿主细胞经遗传修饰以包含编码乙醛脱氢酶的异源核苷酸序列，乙酰化（ADA，E.C.1.2.1.10）和包含使用NADH的HMG-CoA还原酶的MEV途径。 在一些实施方案中，宿主细胞被遗传修饰以包含编码包含乙酰乙酰辅酶A合成酶的ADA和MEV途径的异源核苷酸序列。 在一些实施方案中，经遗传修饰的宿主细胞还包含一个或多个编码磷酸酮醇酶和磷酸转乙酰酶的异源核苷酸序列。 在一些实施方案中，遗传修饰的宿主细胞还包括天然PDH旁路的功能性破坏。 本文描述的组合物和方法提供了用于异源产生乙酰辅酶A衍生的类异戊二烯的能量效率高的均衡路线。

    PROCESS FOR PRODUCTION OF HIGH PURITY BETA-CAROTENE AND LYCOPENE CRYSTALS FROM FUNGAL BIOMASS
    26.
    发明公开
    PROCESS FOR PRODUCTION OF HIGH PURITY BETA-CAROTENE AND LYCOPENE CRYSTALS FROM FUNGAL BIOMASS 有权
    工艺生产高纯度的β-胡萝卜素和蘑菇LYCOPENKRISTALLEN从生物质

    公开(公告)号：EP2571996A2

    公开(公告)日：2013-03-27

    申请号：EP11740723.9

    申请日：2011-05-16

    申请人： Dynadis Biotehc (India) Private Limited

    发明人： JOSEPH, Suresh ,  ANANDANE, Arnaud

    IPC分类号： C12P23/00 ,  C07C403/24

    CPC分类号： C07C403/24 ,  C07C11/02 ,  C12P23/00

    摘要： The present invention relates to a simple and economic method of extracting a crystalline Carotenoid compound, such as Beta-carotene, Lycopene, with a purity of at least 99%. The present invention further describes a process to prepare such a highly pure crystalline Carotenoid compound from microbial biomass, using an Anti-purity compound removal process followed by a mono-solvent extraction method. Further the process describes value addition of the co-products recovered during the extraction process thus resulting in a highly economical industrial method for the production of such high purity crystalline Carotenoids compound.

    METHOD OF PRODUCING ISOPRENOID COMPOUNDS IN YEAST
    27.
    发明公开
    METHOD OF PRODUCING ISOPRENOID COMPOUNDS IN YEAST 审中-公开
    HEFE VERFAHREN ZUR HERSTELLUNG VON ISOPRENOIDVERBINDUNGEN

    公开(公告)号：EP2571991A1

    公开(公告)日：2013-03-27

    申请号：EP11721630.9

    申请日：2011-05-20

    申请人： Evolva SA

    发明人： HANSEN, Jørgen

    IPC分类号： C12N15/52 ,  C12P23/00 ,  C12N1/16 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12

    CPC分类号： C12P23/00 ,  C12N9/1025 ,  C12N9/1241 ,  C12N15/52 ,  C12P5/007 ,  C12Y203/03008 ,  Y02P20/52

    摘要： Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co- expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of S. cerevisiae.

    摘要翻译： 提供能够增加异戊烯基磷酸酯生产的酵母菌株，使萜类化合物生产增加。 使用两种不同策略中的一种或两种来制备具有高异戊烯基磷酸酯可用性的异源酵母菌株，以增加萜类化合物生产的磷酸异戊酯的可用性。 首先，通过共同表达多种甲羟戊酸途径基因类似物，基因的新型异源组合结果，其中一些增加了酵母中异戊烯基磷酸酯的固有可用性。 第二，通过表达非内源性酶ATP柠檬酸裂解酶（ACL），在酿酒酵母的胞质溶胶中产生高度胞质浓度的乙酰辅酶A的积累。